The zebrafish cohesin protein Sgo1 is required for cardiac function and eye development

Kamel, Sarah M.; Broekman, Sanne; Tessadori, Federico; Wijk, Erwin van; Bakkers, Jeroen

doi:10.1002/dvdy.468

Cited by 4 publications

(2 citation statements)

References 37 publications

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“…Moreover, a mutation of the cell cycle gene regulator rbbp4 results in a reduced size of the brain and eyes due to mitotic arrest and the subsequent apoptosis of neural progenitor cells, leading to the loss of differentiated neurons [64]. In another study, the deletion of sgo1, a cohesin regulator, is implicated in developmental defects, including abnormal eye morphology [65]. Additionally, the loss of osgep and tpkrb, tRNA processing factors which have been identified to regulate the maintenance of genomic stability, leads to microcephalic phenotypes with induced apoptosis in zebrafish [66].…”

Section: Neurogenesismentioning

confidence: 99%

Unraveling DNA Repair Processes In Vivo: Insights from Zebrafish Studies

Shin,

Lee

2023

IJMS

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The critical role of the DNA repair system in preserving the health and survival of living organisms is widely recognized as dysfunction within this system can result in a broad range of severe conditions, including neurodegenerative diseases, blood disorders, infertility, and cancer. Despite comprehensive research on the molecular and cellular mechanisms of DNA repair pathways, there remains a significant knowledge gap concerning these processes at an organismal level. The teleost zebrafish has emerged as a powerful model organism for investigating these intricate DNA repair mechanisms. Their utility arises from a combination of their well-characterized genomic information, the ability to visualize specific phenotype outcomes in distinct cells and tissues, and the availability of diverse genetic experimental approaches. In this review, we provide an in-depth overview of recent advancements in our understanding of the in vivo roles of DNA repair pathways. We cover a variety of critical biological processes including neurogenesis, hematopoiesis, germ cell development, tumorigenesis, and aging, with a specific emphasis on findings obtained from the use of zebrafish as a model system. Our comprehensive review highlights the importance of zebrafish in enhancing our understanding of the functions of DNA repair systems at the organismal level and paves the way for future investigations in this field.

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Section: Neurogenesismentioning

confidence: 99%

Unraveling DNA Repair Processes In Vivo: Insights from Zebrafish Studies

Shin,

Lee

2023

IJMS

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“…Accurately measuring FI in zebrafish studies is critical. Previously described methods have included video imaging (e.g., by feeding fish flakes or Paramecia to which fluorescent dyes have been added to measure internalized fluorescence [50,69,73,80,85] or by using photometric assays to measure the orange-red color of fish after feeding brine shrimp [59]) or feed particle or Artemia nauplii consumption counting [56,62,70,72,74]. Other authors only considered the time fish spent feeding [53,65].…”

Section: Zebrafish Feed Intake: How Much Do They Eat?mentioning

confidence: 99%

Zebrafish Feed Intake: A Systematic Review for Standardizing Feeding Management in Laboratory Conditions

Licitra,

Fronte,

Verri

et al. 2024

Biology

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Zebrafish are one of the most used animal models in biological research and a cost-effective alternative to rodents. Despite this, nutritional requirements and standardized feeding protocols have not yet been established for this species. This is important to avoid nutritional effects on experimental outcomes, and especially when zebrafish models are used in preclinical studies, as many diseases have nutritional confounding factors. A key aspect of zebrafish nutrition is related to feed intake, the amount of feed ingested by each fish daily. With the goal of standardizing feeding protocols among the zebrafish community, this paper systematically reviews the available data from 73 studies on zebrafish feed intake, feeding regimes (levels), and diet composition. Great variability was observed regarding diet composition, especially regarding crude protein (mean 44.98 ± 9.87%) and lipid content (9.91 ± 5.40%). Interestingly, the gross energy levels of the zebrafish diets were similar across the reviewed studies (20.39 ± 2.10 kilojoules/g of feed). In most of the reviewed papers, fish received a predetermined quantity of feed (feed supplied). The authors fed the fish according to the voluntary intake and then calculated feed intake (FI) in only 17 papers. From a quantitative point of view, FI was higher than when a fixed quantity (pre-defined) of feed was supplied. Also, the literature showed that many biotic and abiotic factors may affect zebrafish FI. Finally, based on the FI data gathered from the literature, a new feeding protocol is proposed. In summary, a daily feeding rate of 9–10% of body weight is proposed for larvae, whereas these values are equal to 6–8% for juveniles and 5% for adults when a dry feed with a proper protein and energy content is used.

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The zebrafish cohesin protein Sgo1 is required for cardiac function and eye development

Kamel

Broekman

Tessadori

et al. 2022

Developmental Dynamics

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Background: Cohesinopathies is a term that refers to/covers rare genetic diseases caused by mutations in the cohesin complex proteins. The cohesin complex is a multiprotein complex that facilitates different aspects of cell division, gene transcription, DNA damage repair, and chromosome architecture. Shugoshin proteins prevent the cohesin complex from premature dissociation from chromatids during cell division. Patients with a homozygous missense mutation in SGO1, which encodes for Shugoshin1, have problems with normal pacing of the heart and gut.Results: To study the role of shugoshin during embryo development, we mutated the zebrafish sgo1 gene. Homozygous sgo1 mutant embryos display various phenotypes related to different organs, including a reduced heart rate accompanied by reduced cardiac function. In addition, sgo1 mutants are vision-impaired as a consequence of structurally defective and partially nonfunctional photoreceptor cells. Furthermore, the sgo1 mutants display reduced food intake and early lethality. Conclusion:We have generated a zebrafish model of Sgo1 that showed its importance during organ development and function.

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The zebrafish cohesin protein Sgo1 is required for cardiac function and eye development

Cited by 4 publications

References 37 publications

Unraveling DNA Repair Processes In Vivo: Insights from Zebrafish Studies

Unraveling DNA Repair Processes In Vivo: Insights from Zebrafish Studies

Zebrafish Feed Intake: A Systematic Review for Standardizing Feeding Management in Laboratory Conditions

The zebrafish cohesin protein Sgo1 is required for cardiac function and eye development

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