The α₁-antitrypsin/elastase Complex as an Experimental Model for Hemodialysis in Acute Catabolic Renal Failure, Extracorporeal Blood Circulation and Cardiocirculatory Bypass

Abstract

A modified polyethersulphone graft membrane was loaded with antiproteases, with the aim of reducing the active protease blood concentration during hemodialysis in acute catabolic renal failure or cardiopulmonary bypass. As protease/antiprotease system, elastase and alpha1-antitrypsin were used. The concentration of active elastase in aqueous solutions decreased as function of contact time with the membrane, approaching saturation. A 40% loss of elastase activity was obtained at pH 7.4, which was not due to autolysis, which accounted for 5% of the loss. The highest reduction was achieved at pH 9.0 (25% higher than at pH 7.4). The saturation level of elastase decrease, calculated by means of the Einstein equation, was reached after more than 47 minutes. We speculate that a time reduction might be achieved either increasing the concentration of immobilized antiproteases, or increasing the rate of elastase movement across the membranes by hydraulic, osmotic, or temperature gradients. This technology can be applied to hemodialysis, and in extracorporeal blood circulation to promote elastase release.

Osseointegration assessment of chairside argon‐based nonthermal plasma‐treated Ca‐P coated dental implants

“…More recently (4, 5) it has been demonstrated that it is possible to reduce the concentration of active elastase in an aqueous solution interacting with a modified polyethersulfone membrane loaded with α 1 ‐antitrypsin. Elastase was chosen as protease model, because it represents one of the main proteolytic enzymes released during HE or CPB (6, 7).…”

2D modeling and preliminary in vitro investigation of a prototype high gradient magnetic separator for biomedical applications

“…An externally applied field magnetizes the parallel wires which, in turn, generate sufficient local magnetic field gradients within each tubing segment to quantitatively capture the blood-borne magnetic spheres at the inner tubing wall. This biomedical design borrows from and combines two established technologies: first, the high gradient magnetic separation (HGMS) principle, commonly utilized in industry for the separation of waste products [19][20][21] and biomolecule and cell separations [5][6][7][8][9][10][11][12][13][14][15][16][17][18] and second, the biomedical application of extracorporeal blood circulation [22][23][24].…”

Reduction of Active Elastase Concentration by Means of Immobilized Inhibitors: A Novel Therapeutic Approach

“…More recently (4,5) it has been demonstrated that it is possible to reduce the concentration of active elastase in an aqueous solution interacting with a modified polyethersulfone membrane loaded with R 1 -antitrypsin. Elastase was chosen as protease model, because it represents one of the main proteolytic enzymes released during HE or CPB (6,7).…”