TheHsp70 homolog gene,Hsc70t, is expressed under translational control during mouse spermiogenesis

Tsunekawa, Naoki; Matsumoto, Midori; Toné, Shigenobu; Nishida, Takao; Fujimoto, Hirokazu

doi:10.1002/(sici)1098-2795(199904)52:4<383::aid-mrd7>3.0.co;2-z

Cited by 41 publications

(33 citation statements)

References 52 publications

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“…Clear immunological reactivity with the anti-Hsc70t antiserum appeared between steps 11 and 12 of spermatid development and strong reactivity of the cytoplasm of the spermatids persisted through step 13 to the final step 16 [45]. As shown in Fig.…”

Section: Hsc70t Antigen Was Detected In the Spermatids Of Mammals Incmentioning

confidence: 72%

“…Our findings suggest that marsupials and primitive eutherian mammals continue to express the Hsp70 during spermatogenesis in a primitive style different from the expression pattern of other mammals. Our previous study, however, indicated that the anti-Hsc70t antiserum reacts faintly with the Hsp70-2 protein expressed in spermatocytes during mouse spermatogenesis [45]. Thus, there is a possibility that different pattern of the Hsc70t antigen expression in these species may be caused by unmasking of epitopes of the different Hsp70-related molecules.…”

Section: Discussionmentioning

confidence: 88%

“…The BRM-22 antibody recognizes at least five Hsp70-related proteins including both Hsp70-2 and Hsc70 [1]. Our previous study has showed that this antibody also recognizes the Hsc70t protein on Western blot analyses [45].…”

Section: Methodsmentioning

confidence: 99%

“…Antibody: The rabbit anti-mouse Hsc70t antiserum was prepared as described in our previous study [28,45]. Amino acid sequences of different Hsp70 proteins were closely similar, but vary much more in the carboxyl terminal than the amino terminal part [32].…”

Section: Methodsmentioning

confidence: 99%

“…Extensive analyses using knock-out mutant mice of the Hsp70-2 gene revealed that Hsp70-2 is required for synaptonemal complex desynapsis [1,9,10,51]. We have showed that another kind of Hsp70-related protein, Hsc70t, is expressed in late spermatids during spermiogenesis [27,29,45]. The amino acid sequences of mouse Hsc70t gene and human orthologous gene HSPA1L are highly similar to those of heat-inducible Hsp70 genes, but these genes differ in the regulation of their expression [17,30].…”

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confidence: 99%

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Expression of the Spermatid-Specific Hsp70 Antigen is Conserved in Mammals Including Marsupials

Tsunekawa

Nishida

Fujimoto

1999

The Journal of Veterinary Medical Science

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ABSTRACT. The anatomical location of testes in mammals ranges from a location close to that observed in the embryo to a lower position usually involving a pendant scrotum. In scrotal mammals, the abdominal position of the cryptorchid testis, which elevates its temperature, is detrimental to spermatogenesis and causes infertility. Spermatocytes are sensitive but late spermatids are relatively resistant to thermal stress suggesting that the latter might be protected in some way. In general, most organisms express Hsp70 proteins, which play a crucial role in the protection of cells against thermal stress. We have found previously that the Hsc70t protein, a member of the Hsp70 family of proteins, is constitutively expressed in the late spermatids of mice. Here, we have utilized immunohistochemistry with anti-mouse Hsc70t antiserum to examine the expression of the spermatid-specific Hsp70 antigen in the testes of several mammalian species with different degrees of testes migration. Our data indicate that the antigen is conserved in the mammals including marsupials. We also examined whether antigens of Hsp70-related proteins were expressed in non-mammalian vertebrates including not only homoiothermal but also poikilothermal animals. The spermatid-specific Hsp70 antigens were not detectable in the testes of the animals examined. From results of immunohistochemistry with BRM22 monoclonal antibody which reacts broadly with Hsp70 family proteins, however, we revealed constitutive expression of antigens of Hsp70-related proteins in spermatogenic cells of the vertebrates. These results suggest that the expression of spermatid-specific Hsp70 protein may be involved in the developmental pathway during spermiogenesis in mammals rather than in thermotolerance.-KEY WORDS: Hsp70, immunohistochemistry, mammal, spermatogenesis, testis migration.J. Vet. Med. Sci. 61(4): [381][382][383][384][385][386][387][388] 1999 prominent and evolutionary well conserved is the 70 kDa heat shock protein (Hsp70) [26]. Some role of the Hsp70 in cellular response to heat injury is suggested by the temporal correlation between Hsp70 synthesis and the appearance of thermotolerance [22,23,43] and the increased sensitivity to heat when intracellular Hsp70 levels are reduced by antiHsp70 antibodies [38] or by competitive inhibition of Hsp70 mRNA [18]. Most eukaryotes have a large family of at least five Hsp70-related proteins [15,16,32]. A unique pattern of expression of Hsp70 has been revealed during mouse and rat spermatogenesis [2,19,20]. Mouse Hsp70-2 has been shown to be expressed at high levels during meiosis [41]. Extensive analyses using knock-out mutant mice of the Hsp70-2 gene revealed that Hsp70-2 is required for synaptonemal complex desynapsis [1,9,10,51]. We have showed that another kind of Hsp70-related protein, Hsc70t, is expressed in late spermatids during spermiogenesis [27,29,45]. The amino acid sequences of mouse Hsc70t gene and human orthologous gene HSPA1L are highly similar to those of heat-inducible Hsp70 genes, but these genes d...

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Section: Hsc70t Antigen Was Detected In the Spermatids Of Mammals Incmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 88%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Expression of the Spermatid-Specific Hsp70 Antigen is Conserved in Mammals Including Marsupials

Tsunekawa

Nishida

Fujimoto

1999

The Journal of Veterinary Medical Science

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Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 3: Developmental changes in spermatid flagellum and cytoplasmic droplet and interaction of sperm with the zona pellucida and egg plasma membrane

Hermo¹,

Pelletier²,

Cyr³

et al. 2009

Microscopy Res & Technique

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Spermiogenesis constitutes the steps involved in the metamorphosis of spermatids into spermatozoa. It involves modification of several organelles in addition to the formation of several structures including the flagellum and cytoplasmic droplet. The flagellum is composed of a neck region and middle, principal, and end pieces. The axoneme composed of nine outer microtubular doublets circularly arranged to form a cylinder around a central pair of microtubules is present throughout the flagellum. The middle and principal pieces each contain specific components such as the mitochondrial sheath and fibrous sheath, respectively, while outer dense fibers are common to both. A plethora of proteins are constituents of each of these structures, with each playing key roles in functions related to the fertility of spermatozoa. At the end of spermiogenesis, a portion of spermatid cytoplasm remains associated with the released spermatozoa, referred to as the cytoplasmic droplet. The latter has as its main feature Golgi saccules, which appear to modify the plasma membrane of spermatozoa as they move down the epididymal duct and hence may be partly involved in male gamete maturation. The end product of spermatogenesis is highly streamlined and motile spermatozoa having a condensed nucleus equipped with an acrosome. Spermatozoa move through the female reproductive tract and eventually penetrate the zona pellucida and bind to the egg plasma membrane. Many proteins have been implicated in the process of fertilization as well as a plethora of proteins involved in the development of spermatids and sperm, and these are high lighted in this review.

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Complementary DNA cloning of rat spetex‐1, a spermatid‐expressing gene‐1, encoding a 63 kDa cytoplasmic protein of elongate spermatids

Iida¹,

Ichinose²,

Kaneko³

et al. 2004

Molecular Reproduction Devel

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We used differential display in combination with complementary DNA (cDNA) cloning approach to isolate a novel rat gene designated as spetex-1, which had an open reading frame of 1,668-length nucleotides encoding a protein of 556 amino acids. Spetex-1 mRNA was highly expressed in testis, and weekly expressed in lung, intestine, and spleen. Spetex-1 expression in the rat testes was detected first at 3 weeks in postnatal development and continued to be detected up to adulthood. A search in the databases showed that the amino acid sequence of spetex-1 was 82% identical to that of its mouse homologue found in the databases. Both rat spetex-1 and the mouse homologue contained Ser-X (X = His, Arg, or Asn) repeats in the middle portion of the proteins. In situ hybridization revealed that spetex-1 mRNA was expressed in haploid spermatids of step 7-18 within the seminiferous epithelium. Immunohistochemical analysis with confocal laser-scanning microscopy demonstrated that spetex-1 protein was not expressed in spermatogonia, spermatocytes, and round spermatids in adult rat testis, but was specifically detected in the residual cytoplasm of elongate spermatids of step 15-18 as well as in residual bodies engulfed by Sertoli cells. We interpreted these data as a potential role of spetex-1 in spermatogenesis, especially in cell differentiation from late elongate spermatids to mature spermatozoa.

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TheHsp70 homolog gene,Hsc70t, is expressed under translational control during mouse spermiogenesis

Cited by 41 publications

References 52 publications

Expression of the Spermatid-Specific Hsp70 Antigen is Conserved in Mammals Including Marsupials

Expression of the Spermatid-Specific Hsp70 Antigen is Conserved in Mammals Including Marsupials

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 3: Developmental changes in spermatid flagellum and cytoplasmic droplet and interaction of sperm with the zona pellucida and egg plasma membrane

Complementary DNA cloning of rat spetex‐1, a spermatid‐expressing gene‐1, encoding a 63 kDa cytoplasmic protein of elongate spermatids

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