Theoretical analysis of the behavior of a centrally injected band in a homogeneous chromatographic column

Tong, Yun; Smith, Maureen S.; Guiochon, Georges

doi:10.1016/s0021-9673(98)00663-3

Cited by 12 publications

(3 citation statements)

References 24 publications

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“…The effects of radial bed heterogeneity may be avoided somewhat by injecting the sample in the radial centre of the column. The process of undertaking these “central point injections” was first illustrated by Knox and Parcher 20 in the 1960s, and later, a theoretical description of the process was developed by Guiochon and co‐workers 21. The purpose behind central point injections was to limit the sample distribution to the central zone of the column, avoiding the region near the wall where fluid flow is retarded by the higher packing density.…”

Section: Introductionmentioning

confidence: 99%

Active flow management in preparative chromatographic separations: A preliminary investigation into enhanced separation using a curtain flow inlet fitting and segmented flow outlet fitting

Camenzuli

Ritchie

Ladine

et al. 2012

J of Separation Science

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Active flow management in the form of curtain flow sample introduction and segmented outlet flow control has been shown to enable sample to elute through a chromatography column under the principles of the "infinite diameter column". Such an elution process avoids the detrimental effects of the heterogeneity of particle-packed chromatographic columns by injecting the sample directly into the radial core region of the column, thus avoiding wall effects. The process described herein illustrates how the principles of the infinite diameter column can be applied using conventional injection devices suitable for long-term analysis that requires robust protocols. Using this approach, sensitivity in separation was 2.5 times greater than conventional chromatography, yielding a product at twice the concentration. Benefits of curtain flow injection are thus relevant to both preparative-scale and analytical-scale separations.

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Section: Introductionmentioning

confidence: 99%

Active flow management in preparative chromatographic separations: A preliminary investigation into enhanced separation using a curtain flow inlet fitting and segmented flow outlet fitting

Camenzuli

Ritchie

Ladine

et al. 2012

J of Separation Science

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“…Here the chromatography column has been modified to include a specially designed header fitting that allows for sample to be introduced to the top of the column as a central injection. 11,12 A curtain flow of mobile phase restricts the solute migration to the central region of the bed and a special outlet fitting separates sample eluting from the central radial section of the column from sample that elutes in the wall region of the column. The principles of the infinite diameter column are applied, 11,12 but in a manner which facilitates automated injection.…”

Section: Introductionmentioning

confidence: 99%

“…11,12 A curtain flow of mobile phase restricts the solute migration to the central region of the bed and a special outlet fitting separates sample eluting from the central radial section of the column from sample that elutes in the wall region of the column. The principles of the infinite diameter column are applied, 11,12 but in a manner which facilitates automated injection.…”

Section: Introductionmentioning

confidence: 99%

The design of a new concept chromatography column

Camenzuli

Ritchie

Ladine

et al. 2011

Analyst

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Active Flow Management is a new separation technique whereby the flow of mobile phase and the injection of sample are introduced to the column in a manner that allows migration according to the principles of the infinite diameter column. A segmented flow outlet fitting allows for the separation of solvent or solute that elutes along the central radial section of the column from that of the sample or solvent that elutes along the wall region of the column. Separation efficiency on the analytical scale is increased by 25% with an increase in sensitivity by as much as 52% compared to conventional separations.

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Large‐Scale Chromatography Columns, Modeling Flow Distribution

Fang

2010

Encyclopedia of Industrial Biotechnology

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Liquid chromatography has become an indispensable tool in the pharmaceutical and biotechnology industries for preparative and large‐scale purifications to obtain high purity biopharmaceutical products. However, imperfect packing of the column bed and complex rheological behaviors of resin particles pose a great challenge for any modeling effort. A systematic study, based on rigorous physics, which attempts to understand the intrinsic complicated interrelationships between column packing, hardware design, flow distribution, and separation efficiency would be of great help to chromatography practitioners. Seeing this need, this article makes an effort to perform a systematic study of the chromatography processes from start to finish and to provide mathematical models and case studies for each stage. First, the author discusses the challenges in scaling up chromatography column packing and examines the wall effect through a dimensional analysis, then follows it by introducing a one‐dimensional model, describing column packing and scale‐up. Next, the author presents a case study on a commercial scale column with a special headplate design and investigates the impact of hardware design on flow distribution, using the technique of computational fluid dynamics (CFD). Finally, this article presents simulations on the transport of elute and computation on the height equivalent to a theoretic plate (HETP) in a modeled, large column set for no retention, ion exchange, and affinity systems. The author discovers that the hardware design exerts a great impact on the flow distribution in the columns and thus affects the column separation efficiency to a large extent.

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Theoretical analysis of the behavior of a centrally injected band in a homogeneous chromatographic column

Cited by 12 publications

References 24 publications

Active flow management in preparative chromatographic separations: A preliminary investigation into enhanced separation using a curtain flow inlet fitting and segmented flow outlet fitting

Active flow management in preparative chromatographic separations: A preliminary investigation into enhanced separation using a curtain flow inlet fitting and segmented flow outlet fitting

The design of a new concept chromatography column

Large‐Scale Chromatography Columns, Modeling Flow Distribution

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