Theoretical and experimental characterizations of gigahertz acoustic streaming in microscale fluids

Cui, Weiwei; Pang, Wei; Yang, Yang; Li, Tiechuan; Duan, Xuexin

doi:10.1016/j.npe.2019.03.004

Cited by 27 publications

(11 citation statements)

References 25 publications

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“…Once the device was xed, the strength and distribution of the acoustic streaming were mainly determined by the geometry of the microchannel. 52,56,57 We then calculated the distribution of the acoustic eld under different geometric con nements, as shown in Supplementary Fig. S3.…”

Section: Working Principle and Design Of The Steast Systemmentioning

confidence: 99%

Manipulation of Single Cells via a Stereo Acoustic Streaming Tunnel (SteAST)

Duan

Yang

Zhang

et al. 2022

Preprint

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At the single-cell level, cellular parameters, gene expression and function are assayed on an individual but not population-average basis. Essential to observing and analyzing the heterogeneity and behavior of these cells/clusters is the ability to prepare and manipulate individual. Here, we demonstrate a versatile microsystem, a stereo acoustic streaming tunnel, which is triggered by ultrahigh-frequency bulk acoustic waves and highly confined by a microchannel. We thoroughly analyze the generation and feature of stereo acoustic streaming to develop a virtual tunnel for observation, pretreatment and analysis of cells for different single-cell applications. 3D reconstruction, dissociation of clusters, selective trapping/release, in-situ analysis and pairing of single cells with barcode gel beads were demonstrated. In order to further verify the reliability and robustness of this technology in complex bio-samples, separation of circulating tumor cells based on both physics and immunity from undiluted blood was achieved. With the rich selection of handling modes, the platform has the potential to be a full-process microsystem from pretreatment to analysis and used in numerous fields, such as in vitro diagnosis, high-throughput single-cell sequencing and drug development.

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Section: Working Principle and Design Of The Steast Systemmentioning

confidence: 99%

Manipulation of Single Cells via a Stereo Acoustic Streaming Tunnel (SteAST)

Duan

Yang

Zhang

et al. 2022

Preprint

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“…Surface acoustic waves in the frequency range of 10's MHz to 10's GHz [150,[197][198][199][200][201] have gained increased attention due to their extensive value in manipulation, separation, and concentration of organisms, [202][203][204] cells, [205][206][207][208][209][210][211][212][213][214][215][216][217][218] particles, [140,212,215,[219][220][221][222][223][224][225][226][227][228][229] and nanomaterials. [230][231][232] Recently, surface acoustic waves have been leveraged to develop various technologies for sonoporation.…”

Section: High-frequency Surface Acoustic Wave-based Technologiesmentioning

confidence: 99%

“…It would be valuable to see if this technology could be applied to primary cells. With the success of using acoustic streaming induced by 80 MHz surface acoustic waves for intracellular delivery, we envision that the potential of using acoustic streaming induced by hyper-frequency >1 GHz surface acoustic waves [150,[197][198][199][200][201] for sonoporation is very plausible.…”

Section: High-frequency Surface Acoustic Wave-based Technologiesmentioning

confidence: 99%

Sonoporation: Past, Present, and Future

Rich

Tian

Huang

2021

Adv Materials Technologies

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only contribute to fundamental studies in areas of biology, bioengineering, and medicine, but also benefit several life-changing technologies, such as cell-based therapies, protein therapeutics, vaccines, as well as the reprogramming of cells. [22] Among the current delivery approaches, the viral vector-based approach is often considered the gold standard for the delivery of nucleic acids. [22] However, due to notable weaknesses such as biohazards, inconsistent results, and labor-intensive manufacturing, [22][23][24] physical permeabilization approaches are being pursued, [1][2][3][4][5][6][7][8][9][10][11][12]22] including electroporation, [25,26] hydroporation, [27][28][29][30][31][32] mechanoporation, [25,[32][33][34][35][36][37][38][39][40] and sonoporation. [41,42] These approaches are noted as advanced due to their high throughputs, cost-effectiveness, and universal nature to deliver a variety of cargos regardless of cell type. [3] Of the physical permeabilization approaches, sonoporation wields great potential and has been demonstrated as an efficacious technology for delivering a variety of functional cargos, such as biomolecular drugs, proteins, and plasmids, to different types of cells, such as cancer, immune, and stem cells. [43][44][45][46][47][48] Sonoporation was discovered in the 1980s, a golden age of intracellular delivery, in which quite a few physical permeabilization methods were invented. [49][50][51][52][53][54][55][56][57][58] Sonoporation enhances the intracellular delivery of cargos into cells by employing acoustic waves to disrupt their membranes. [22,[59][60][61][62] Traditional sonoporation approaches typically rely on ultrasound-induced bubble dynamic behaviors, such as inertial cavitation, noninertial (stable) cavitation, and bubble translation. [49][50][51][52] However, the bubble-based sonoporation approaches usually require special contrast agents, [63][64][65][66][67][68] which adds a new dimension of complexity and cost to the use of sonoporation. Moreover, bubble-based approaches have a high chance of inducing irreversible cell damage, lowering the cell viability, as well as reducing the effectiveness of delivered cargos. [69][70][71][72][73] Therefore, several novel non-bubble-based sonoporation approaches, such as those based on acoustic radiation force and acoustic streaming-induced shear force, are being developed to overcome the limitations of bubble-based sonoporation approaches. Although there are several review papers on the mechanisms and applications of bubble-based sonoporation, [61,62,[74][75][76][77][78][79][80][81][82][83][84][85][86][87][88] they have neglected some of the newly developed mechanisms. Therefore, this review article covers recent innovations in bubble-based and especially in non-bubble-based sonoporation. The following sections review the sonoporation mechanisms, A surge of research in intracellular delivery technologies is underway with the increased innovations in cell-based therapies and cell reprogramming. Particularly, physical cell membrane p...

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“…Recently, we developed a novel hydrodynamic approach for transient cellular poration based on high frequency acoustic stimulation . Controlled high speed acoustic streaming flow speeds (>m/s) was generated using our gigahertz-frequency (GHz) acoustic resonator, , inducing shear stress that triggered gentle deformation of cell membranes and temporarily improved cell permeability . Compared to other physical methods, acoustic streaming features a simple setup and robust instrumentation and is generally less invasive.…”

Section: Introductionmentioning

confidence: 99%

Cytosolic Delivery of Functional Proteins In Vitro through Tunable Gigahertz Acoustics

Pan

Jeon

Luther

et al. 2020

ACS Appl. Mater. Interfaces

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Intracellular delivery is essential to therapeutic applications such as genome engineering and disease diagnosis. Current methods lack simple, noninvasive strategies and are often hindered by long incubation time or high toxicity. Hydrodynamic approaches offer rapid and controllable delivery of small molecules, but thus far have not been demonstrated for delivering functional proteins. In this work, we developed a robust hydrodynamic approach based on gigahertz (GHz) acoustics to achieve rapid and noninvasive cytosolic delivery of biologically active proteins. With this method, GHz-based acoustic devices trigger oscillations through a liquid medium (acoustic streaming), generating shear stress on the cell membrane and inducing transient nanoporation. This mechanical effect enhances membrane permeability and enables cytosolic access to cationic proteins without disturbing their bioactivity. We evaluated the versatility of this approach through the delivery of cationic fluorescent proteins to a range of cell lines, all of which displayed equally efficient delivery speed (≤20 min). Delivery of multiple enzymatically active proteins with functionality related to apoptosis or genetic recombination further demonstrated the relevance of this method.

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Theoretical and experimental characterizations of gigahertz acoustic streaming in microscale fluids

Cited by 27 publications

References 25 publications

Manipulation of Single Cells via a Stereo Acoustic Streaming Tunnel (SteAST)

Manipulation of Single Cells via a Stereo Acoustic Streaming Tunnel (SteAST)

Sonoporation: Past, Present, and Future

Cytosolic Delivery of Functional Proteins In Vitro through Tunable Gigahertz Acoustics

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