We present an acoustic microfluidic mixing approach via acousto-mechanically induced micro-vortices sustained by localized ultrahigh frequency (UHF) acoustic fields. A micro-fabricated solid-mounted thin-film piezoelectric resonator (SMR) with a frequency of 1.54 GHz has been integrated into microfluidic systems. Experimental and simulation results show that UHF-SMR triggers strong acoustic field gradients to produce efficient and highly localized acoustic streaming vortices, providing a powerful source for microfluidic mixing. Homogeneous mixing with 87% mixing efficiency at a Peclet number of 35520 within 1 ms has been achieved. The proposed strategy shows a great potential for microfluidic mixing and enhanced molecule transportation in minimized analytical systems.

We demonstrate a label-free, high-throughput cell separation method via a SSAW-based acoustofluidic device with a serpentine channel for clinical diagnosis and point-of-care analysis.

Nanopore sensors are expected to be one of the most promising next generation sequencing technologies, with label-free, amplification-free and high-throughput features, as well as rapid detections and low cost. Solid-state nanopores have been widely explored due to their diverse fabrication methods and CMOS compatibility. Here, we highlight the fabrication methods of solid-state nanopores, including the direct opening and the tuning methods. In addition, molecular translocation developments, DNA sequencing and protein detections are summarized. Finally, the latest progress relating to solid-state nanopores is discussed, which helps to offer a comprehensive understanding of the current situation for solid-state nanopore sensors.

In this study, we present a nanoscale acoustofluidic trap (AFT) that enables a simple, no-wash, and practical approach for biosensing applications.