Theoretical investigations of a novel microfluidic cooling/warming system for cell vitrification cryopreservation

Zhou, Xiaoming; Liu, Zhong; Liang, Xin; Shu, Zhiquan; Du, Pengfei; Gao, Dayong

doi:10.1016/j.ijheatmasstransfer.2013.06.022

Cited by 18 publications

(44 citation statements)

References 27 publications

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“…Furthermore, preventing cytotoxicity from high CPA concentrations required to decrease critical cooling rates necessary for vitreous cryopreservation is of importance (Fahy and Wowk, 2015). Unprecedented successes in cell cooling and warming have been accomplished with emerging novel microfluidic platforms which have intrinsic advantages, especially for cell-specific manipulation and strong controllability for rapid thermal responses (Huang et al, 2015; Lai et al, 2015a; Pyne et al, 2014; Vom et al, 2013; Zhou et al, 2013; Zhou et al, 2015). …”

Section: Fundamentals Of Cryopreservationmentioning

confidence: 99%

Microfluidics for cryopreservation

Zhao

2017

Biotechnology Advances

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Cryopreservation has utility in clinical and scientific research but implementation is highly complex and includes labor-intensive cell-specific protocols for the addition/removal of cryoprotective agents and freeze-thaw cycles. Microfluidic platforms can revolutionize cryopreservation by providing new tools to manipulate and screen cells at micro/nano scales, which are presently difficult or impossible with conventional bulk approaches. This review describes applications of microfluidic chips in cell manipulation, cryoprotective agent exposure, programmed freezing/thawing, vitrification, and in situ assessment in cryopreservation, and discusses achievements and challenges, providing perspectives for future development.

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Section: Fundamentals Of Cryopreservationmentioning

confidence: 99%

Microfluidics for cryopreservation

Zhao

2017

Biotechnology Advances

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“…Vitrification experiments for cryopreservation are usually conducted by plunging vitrification devices with cell suspensions into liquid nitrogen (LN 2 ) [6]. Owing to the large temperature difference between sample and LN 2 , this procedure is commonly accompanied by film boiling of LN 2 on device surface [7, 8], which consequently blocks heat transfer surrounding the sample and leads to incomplete vitrification. Some researchers have worked on enhancing heat transfer during cooling in vitrification cryopreservation [8–10].…”

Section: Introductionmentioning

confidence: 99%

“…Zhou et al . utilized micro-channel array on the surface of vitrification device and reported that an ultra-high heat transfer coefficient can be obtained [8, 10]. However, to our knowledge, little research has been done on eliminating film boiling regime.…”

Section: Introductionmentioning

confidence: 99%

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Modeling and experimental studies of enhanced cooling by medical gauze for cell cryopreservation by vitrification

Zhang

Zhao

Hossain

et al. 2017

International Journal of Heat and Mass Transfer

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Vitrification is considered as an important alternative approach to traditional slow freezing method for cryopreservation of cells. A typical cell vitrification procedure involves a non-equilibrium cooling process commonly accomplished in liquid nitrogen, while in which film boiling is believed to greatly hinder heat transfer surrounding the sample, resulting in incomplete vitrification or a much higher critical concentration. In this study, we developed a simple while effective approach, wrapping traditional French-type straw with medical gauze, to greatly enhance convective heat transfer during cooling by suppress film boiling. We further established a coupled heat transfer model for cooling and warming of cell suspensions to investigate the inherent thermodynamic mechanism in this approach. The model describes both the macroscale thermal distributions in extracellular solution and the microscale ice crystallization inside the cells. The simulation indicated that straws wrapped with medical gauze would increase cell survival subject to vitrification cryopreservation by significantly increasing the cooling rate to inhibit intracellular ice formation (IIF). Our experiments on human umbilical vein endothelial cells (HUVECs) further confirmed the predictions in that the cell survival rate was significantly increased by wrapping straws with medical gauze.

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“…Cryovials are necessary equipment used in cryopreservation. Cryovials can be used to cryopreserve samples with a volume of 0.5e4.5 mL at À196 C for long-term storage [2,9]. Traditionally, the sample is taken out of the cryovial after a long rewarming process in a 37 C water bath [10].…”

Section: Introductionmentioning

confidence: 99%