Therapeutic deferoxamine and deferiprone monitoring in β‐thalassemia patients’ plasma by field‐amplified sample injection and sweeping in capillary electrophoresis

Abstract

One CE method was established for detecting deferoxamine (DFO) and deferiprone (DFR) in plasma. For β-thalassemia patients, DFO and DFR are major medicines to treat the iron overload caused by blood transfusion. Field-amplified sample injection combined with sweeping was used for sensitivity enhancement in CE. This method was performed on an uncoated fused-silica capillary. After liquid-liquid extraction, the plasma samples were electrokinetically injected into capillary at +10 kV for 180 s. The phosphate buffer (100 mM) containing 50 mM triethanolamine was used as the BGE (pH 6.6). Separation buffer was phosphate buffer (100 mM, pH 3.0) containing 150 mM SDS. This method showed good linearity (r ≥ 0.9960). Precision and accuracy were evaluated by the results of RSD and relative error of intrabatch and interbatch analyses, and all of the absolute values were less than 6.12%. The LODs (S/N = 3) were 200 ng/mL for DFO, and 25 ng/mL for DFR. The LOQ (S/N = 10) of DFO and DFR were 600 and 75 ng/mL, respectively. This method was applied for clinical applications of five β-thalassemia patients.

Recent progress of sample stacking in capillary electrophoresis (2016–2018)

“…Lin et al. combined FASI and sweeping for simultaneous analysis of deferoxamine and deferasirox in human plasma after LLE. The BGE comprised 100 mM sodium phosphate buffer with 50 mM triethanolamine at pH 6.6 and the separation buffer was 100 mM sodium phosphate at pH 3.0 with 150 mM SDS.…”

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2016–2018)

“…FASI‐sweeping was also applied to deferoxamine and deferiprone in whole blood , 5‐nitroimidazoles in egg samples , chlorpheniramine in rat plasma , methamphetamine in hair samples , and glycopyrrolate stereoisomers in rat plasma . To suit the complex samples for FASI, an adequate procedure of sample preparation was required.…”