Therapeutic Effect and Mechanism of Oxytropis falcata Gel on Deep Second‐Degree Burn in Rats

Abstract

Oxytropis falcata has long been used to treat inflammation, sores, and bleeding in Tibet. However, the burn remedy and underlying molecular mechanisms are not well understood. This study is aimed at assessing the effect of Oxytropis falcate gel (OFG) on deep second-degree burn rats and exploring its mechanism. Wistar rats with second-degree burn were treated with OFG and silver sulfadiazine. Immunohistochemical detections for EGF and VEGF were performed, and ELISA detections for EGF, VEGF, p38, and IL-1β in serum were determined. Rats treated with OFG (25, 50 g/kg) consisted of the major rhamnocitrin-3-O-β-neohesperidoside significantly accelerated incrustation (P < 0.001) and decrustation (P < 0.001). According to HE staining, edema and infiltration of inflammatory cells decrease apparently with good hyperplasia and incrustation in administration groups (7 d). The expressions of EGF and CD34 in OFG (25, 50 g/kg) treatment increased obviously from immunohistochemical assessment (7 d). Serum EGF expression reached 321.27 ± 7.20 ng/mL by OFG treatment, while p38 (P < 0.05) and IL-1β (P < 0.05) levels were significantly lower than the model and vehicle groups from day 1 to day 7. OFG possesses potential wound healing activities. The mechanism may be related to the increasing of biosynthesis and the releasing of EGF and CD34 and the decreasing p38 and IL-1β levels.

Analysis of the Curative Effect and Influencing Factors of Collagen Sponge Combined with Autologous Skin Graft in the Treatment of Deep Burn Patients

“…It is one of the most common traumatic diseases in clinics, which can cause great changes in the pathophysiology of patients, and its disability rate and mortality rate are high. It has become one of the major public health problems in the world [ 1 , 2 ]. Clinically, the main cause of death of burn patients is often the infection caused by a large number of necrotic tissues attached to the wound surface.…”

7-methoxyflavanone alleviates neuroinflammation in lipopolysaccharide-stimulated microglial cells by inhibiting TLR4/MyD88/MAPK signalling and activating the Nrf2/NQO-1 pathway

“…To investigate whether the pretreatment with 7MF affected the viability of LPS-treated cells, BV2 cells were treated with different concentrations (10,20 or 40 µM) of 7MF for 6 h before the addition of 100 ng/ml LPS. After that, cells were incubated for 24 h, and cell viability was evaluated as described above.…”

“…The viabilities of BV2 cells after a 24-h treatment with different concentrations (10,20 or 40 µM) of 7MF alone, or together with 100 ng/ml LPS, were measured. Results showed unaffected cell viabilities (Figure 1b), and therefore, the maximal working concentration of 7MF was set as 40 µM in subsequent experiments.…”

“…[18] The in-vivo anti-inflammatory effects of extracts of O. falcate have also been reported. [19,20] However, there is no report about the effect of 7MF on neuroinflammation reactions. This study is intended to investigate the antineuroinflammatory potential of 7MF.…”

“…It has been reported that 7MF has antiproliferative activity against breast cancer cells; inhibitory activity against cytochrome P450 aromatase and monoamine oxidase‐B; and antioxidant activity in H 2 O 2 ‐induced SH‐SY5Y cells . The in‐vivo anti‐inflammatory effects of extracts of O. falcate have also been reported . However, there is no report about the effect of 7MF on neuroinflammation reactions.…”