Therapeutic effect of Arctium lappa in Schistosoma haematobium associated kidney disturbance: biochemical and molecular effects

Abstract

Schistosoma haematobium (S. haematobium) infection has been found to be strongly associated with bladder cancer, which necessitates for discover of a natural new therapeutic agent. The aim of this study was to evaluate the therapeutic effect of Arctium lappa seed extract in S. haematobium associated kidney disturbance. Forty male albino mice were used and divided into four equal groups; group 1 control includes non-infected healthy mice, groups 2, 3 and 4 subcutaneous infected with S. haematobium cercariae. Groups 3 co-treated daily with oral dose of A. lappa seed extract (300 mg/kg, bwt) for 15 days in the same time of S. haematobium infection. Groups 4 post-treated daily for 15 days with oral dose of A. lappa seed extract (300 mg/kg, bwt) after 15 days of S. haematobium infection. The results obtained revealed that S. haematobium significantly decreased kidney weight and serum sodium, potassium and chloride, but increased urinary volume, urinary excretion of sodium, potassium and chloride, serum urea, creatinine and uric acid. Schistosoma haematobium also significantly decreased kidney superoxide dismutase, glutathione peroxidase and reduced glutathione levels while increased kidney lipid peroxidation level. Co-and post-treatment with A. lappa seed extract restore all the above parameters to approach the normal values. These results were supported with histopathological examinations. In conclusion, A. lappa seed extract has therapeutic effect in kidney disturbance caused by S. haematobium where co-treatment of A. lappa seed extract was more effective than post-treatment of the extract.

Evaluation of sodium fluoride toxicity in Schistosoma infected snails: assessment of antioxidants, antiapoptotic, hypoprotein and hypocholesterol activities

“…Our results are also in accordance with the previously reported decrease in GPx activity after Schistosoma infection due to the interaction of Schistosoma with the essential selenocysteine moiety of the enzyme (Koriem et al 2014(Koriem et al , 2015.…”

“…Our results are in accordance with the previously reported increase in lipid peroxidation in response to Schistosoma (Koriem et al 2014(Koriem et al , 2015. Concomitant reduction of GSH levels might have hampered the decomposition of lipid peroxides in Schistosoma infected snails.…”

“…It is the major health risk in the rural areas of China and South East Asia and continues to rank high in other developing countries (Bichler et al 2001). The disease is diagnosed either by the presence of blood in the urine or, in the case of intestinal schistosomiasis, by initially atypical symptoms which can lead to serious complications involving the hepatocellular carcinoma (ElTonsy et al 2013) and kidney failure (Koriem et al 2015). The life cycles of all five human schistosomes are broadly similar: parasite eggs are released into the environment from infected individuals, hatching on contact with fresh water to release the free-swimming miracidium.…”

In vivo and In vitro of Arctiin Schistosomicidal Activity

“…Research of natural products has provided remarkable new drugs or drug leads to combat several diseases [14][15][16][17][18].…”

“…Furthermore, it was shown that the fruits extract of A. lappa improved kidney function in mice infected with S. haematobium [14].…”