Therapeutic effect of astragaloside-IV on bradycardia is involved in up-regulating klotho expression

Qiu, Xuejia; Guo, Qiaoling; Xiong, Wei; Xia, Yang; Tang, Yiqun

doi:10.1016/j.lfs.2015.11.021

Cited by 14 publications

(6 citation statements)

References 30 publications

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“…These contradictions are related to technical limitations, measurement methods and measuring different parts of the heart. However, Klotho had been shown to be expressed at the sinoatrial node in mouse hearts, but few studies have looked at its expression patterns and function in this tissue (Qiu, Guo, Xiong, Yang, & Tang, 2016; Takeshita et al., 2004). Also the detection of Klotho protein in heart tissue, at a similar molecular size to renal Klotho (Olauson et al., 2017), and the report by Corsetti et al.…”

Section: Discussionmentioning

confidence: 99%

High‐intensity interval training increases myocardial levels of Klotho and protects the heart against ischaemia–reperfusion injury

Ramez

Ramezani

Nasirinezhad

et al. 2020

Experimental Physiology

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Cardiovascular disease, especially coronary artery disease, remains a major cause of morbidity and mortality in the world, and ischaemia-reperfusion (IR) insult is the main pathological cause leading to death. Exercise training is associated with a reduced risk of cardiovascular disease and the development of cardioprotection against IR injury. Therefore, the purpose of this study was to investigate the effect of preconditioning with high-intensity interval training (HIIT) on myocardial and plasma levels of Klotho and its related axes as novel mechanisms of exercise-induced cardioprotection against IR injury. Seventy male Wistar rats were randomly divided into five groups of control, HIIT, sham, IR and HIIT group that underwent IR injury (H-IR). The training group performed five sessions of HIIT on the treadmill. The cardiac IR injury was induced by ligation of the left anterior descending coronary artery for 30 min followed by 24 h reperfusion. Infarct size and histopathological assessment of cardiac tissues were determined through Evans Bluetriphenyltetrazolium chloride and haematoxylin-eosin staining, respectively. We investigated lipid peroxidation and markers of cardiac injury, antioxidant enzymes and the plasma levels of Klotho using enzyme-linked immunosorbent assays. Also, myocardial levels of Klotho and TRPC6 expression were determined by western blot assays. The results demonstrated a significant increase in myocardial and plasma levels of Klotho following HIIT and a significant decrease during IR injury. Myocardial TRPC6 channel expression increased following IR. HIIT also prevented a significant reduction of Klotho during IR and consequently reduced the expression of the TRPC6 channel in the H-IR group compared with the IR group. Furthermore, HIIT decreased the infarct size, cardiac injury, lipid peroxidation, lactate dehydrogenase, creatine kinase myocardial band and cardiac troponin-I, and improved total antioxidant capacity and catalase, superoxide dismutase and glutathione peroxidase activities following IR injury. The findings of the present study suggest that HIIT improves cardioprotection against IR injury and reduces cardiac damages through an increase in myocardial and plasma levels of Klotho and its related axes (TRPC6 and antioxidant defence). These findings can help to develop preventive and therapeutic approaches. K E Y W O R D SAntioxidant defence, cardioprotection, high-intensity interval training, Klotho, TRPC6 channels wileyonlinelibrary.com/journal/eph Experimental Physiology. 2020;105:652-665.

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Section: Discussionmentioning

confidence: 99%

High‐intensity interval training increases myocardial levels of Klotho and protects the heart against ischaemia–reperfusion injury

Ramez

Ramezani

Nasirinezhad

et al. 2020

Experimental Physiology

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“…The expression of α-klotho was modulated by several physiological and pathological factors, including acute inflammatory stress, oxidative stress, diabetes mellitus, hypertension, and chronic renal failure (Ohyama et al, 1998;Utsugi et al, 2000;Moreno et al, 2011;Asai et al, 2012;Qiu et al, 2016;Poelzl et al, 2018). Also, it has been reported that the serum level of α-klotho was increased following the acute exhaustive exercise training (Sven-Jean et al, 2018), but the source of serum α-klotho was unclear.…”

Section: Introductionmentioning

confidence: 99%

α-Klotho Expression in Mouse Tissues Following Acute Exhaustive Exercise

Rao

Zheng

et al. 2019

Front. Physiol.

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α-Klotho, a multifunctional protein, has been demonstrated to protect tissues from injury via anti-oxidation and anti-inflammatory effects. The expression of α-klotho is regulated by several physiological and pathological factors, including acute inflammatory stress, oxidative stress, hypertension, and chronic renal failure. Exhaustive exercise has been reported to result in tissue damage, which is induced by inflammation, oxidative stress, and energy metabolism disturbance. However, little is known about the effects of exhaustive exercise on the expression of α-klotho in various tissues. To determine the effects, the treadmill exhaustion test in mice was performed and the mice were sacrificed at different time points following exhaustive exercise. Our results confirmed that the full-length (130 kDa) and shorter-form (65 kDa) α-klotho were primarily expressed in the kidneys. Moreover, we found that, except for the kidneys and brain, other tissues primarily expressed the shorter-form α-klotho, including liver, which was in contrast to previous reports. Furthermore, the shorter-form α-klotho was decreased immediately following the acute exhaustive exercise and was then restored to the pre-exercise level or even higher levels in the next few days. Our results indicate that α-klotho may play a key role in the body exhaustion and recovery following exhaustive exercise.

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“…Our published data showed that ASG-Ⅳ can effectively protect sinus node function and relieve bradycardia by up-regulating klotho expression (Qiu et al, 2016).…”

Section: Introductionmentioning

confidence: 87%