Therapeutic effect of baicalin on experimental autoimmune encephalomyelitis is mediated by SOCS3 regulatory pathway

Zhang, Yuan; Li, Xing; Ćirić, Bogoljub; Ma, Cun‐Gen; Gran, Bruno; Rostami, Abdolmohamad; Zhang, Guang‐Xian

doi:10.1038/srep17407

Cited by 73 publications

(55 citation statements)

References 50 publications

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“…All efforts were tried to minimize the suffering of mice. EAE was induced according to a previous protocol as described with minor modifications . Briefly, mice were immunized with 150 µg myelin oligodendrocyte glycoprotein (MOG) 35–55 peptide (Genscript, Nanjing, China), and emulsified in complete Freund's adjuvant (Sigma‐Aldrich, St Louis, MO, USA) containing 4 mg mL −1 Mycobacterium tuberculosis H37Ra (Difco, Lawrence, KS, USA).…”

Section: Methodsmentioning

confidence: 99%

Treatment with 6‐Gingerol Regulates Dendritic Cell Activity and Ameliorates the Severity of Experimental Autoimmune Encephalomyelitis

Han

et al. 2019

Molecular Nutrition Food Res

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Scope: Multiple sclerosis (MS) is an inflammatory demyelinating autoimmune disorder, with increasing incidence worldwide but unknown etiology. 6-Gingerol (6-GIN), a major dietary compound found in ginger rhizome, has immunomodulatory activity. However, its role in autoimmune diseases, as well as the underlying mechanisms, are unclear. In this study, it is evaluated if 6-GIN can effectively ameliorate the clinical disease severity of experimental autoimmune encephalomyelitis, an animal model of MS. Methods and results: Clinical scores of experimental autoimmune encephalomyelitis (EAE) mice are recorded daily. Inflammation of periphery and neuroinflammation of EAE mice are determined by flow cytometry analysis, ELISA, and histopathological analysis, and results show that 6-GIN significantly inhibits inflammatory cell infiltration from the periphery into the central nervous system and reduces neuroinflammation and demyelination. Flow cytometry analysis, ELISA, and quantitative PCR show that 6-GIN could suppress lipolysaccharide-induced dendritic cell (DC) activation and induce the tolerogenic DCs. Immunoblot analysis reveals that the phosphorylation of nuclear factor-κB and mitogen-activated protein kinase, two critical regulators of inflammatory signaling, are significantly inhibited in 6-GIN-treated DCs. Conclusion: The results of this study demonstrate that 6-GIN has significant potential as a novel anti-inflammatory agent for the treatment of autoimmune diseases such as MS via direct modulatory effects on DCs.

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Section: Methodsmentioning

confidence: 99%

Treatment with 6‐Gingerol Regulates Dendritic Cell Activity and Ameliorates the Severity of Experimental Autoimmune Encephalomyelitis

Han

et al. 2019

Molecular Nutrition Food Res

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“…Differentiation of Th1 and Th17 cells was induced in vitro following previously described protocols53. Briefly, single-cell suspensions derived from spleen of normal female C57BL/6 mice (6–8 week) were purified by negative selection with a mouse CD4 + T Cell Isolation Kit II (Miltenyi Biotec).…”

Section: Methodsmentioning

confidence: 99%

FSD-C10, a Fasudil derivative, promotes neuroregeneration through indirect and direct mechanisms

Lǐ

Xie

Zhang

et al. 2017

Sci Rep

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FSD-C10, a Fasudil derivative, was shown to reduce severity of experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS), through the modulation of the immune response and induction of neuroprotective molecules in the central nervous system (CNS). However, whether FSD-C10 can promote neuroregeneration remains unknown. In this study, we further analyzed the effect of FSD-C10 on neuroprotection and remyelination. FSD-C10-treated mice showed a longer, thicker and more intense MAP2 and synaptophysin positive signal in the CNS, with significantly fewer CD4+ T cells, macrophages and microglia. Importantly, the CNS of FSD-C10-treated mice showed a shift of activated macrophages/microglia from the type 1 to type 2 status, elevated numbers of oligodendrocyte precursor cells (OPCs) and oligodendrocytes, and increased levels of neurotrophic factors NT-3, GDNF and BDNF. FSD-C10-treated microglia significantly inhibited Th1/Th17 cell differentiation and increased the number of IL-10+ CD4+ T cells, and the conditioned medium from FSD-C10-treated microglia promoted OPC survival and oligodendrocyte maturation. Addition of FSD-C10 directly promoted remyelination in a chemical-induced demyelination model on organotypic slice culture, in a BDNF-dependent manner. Together, these findings demonstrate that FSD-C10 promotes neural repair through mechanisms that involved both immunomodulation and induction of neurotrophic factors.

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“…Flavonoids inhibit STAT signaling through the induction of SOCS3, and they inhibit RhoA GTPases, thus impairing cytoskeletal changes. 147,153 While the flavonoids present in a Western diet may have some capacity to modulate disease mechanisms in MS, flavonoids that are less abundant may have a higher potential to modulate CNS inflammation. Here, the flavonoid subclass of flavones may be particularly effective.…”

Section: Flavonoidsmentioning

confidence: 99%

Western lifestyle and immunopathology of multiple sclerosis

Matveeva

Bogie

Hendriks

et al. 2018

Annals of the New York Academy of Sciences

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There is increasing evidence for a sudden and unprecedented rise in the incidence of multiple sclerosis (MS) in Westernized countries over the past decades, emphasizing the role of environmental factors. Among many candidates, rapid changes in dietary habits seem to play a role in the pathogenesis of MS. Here, we summarize and discuss the available evidence for the role of dietary nutrients, such as table salt, fatty acids, and flavonoids, in the development and pathogenesis of MS. We also discuss new and emerging risk factors accompanying Western lifestyle, such as shift work, sleep, and circadian disruption.

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Therapeutic effect of baicalin on experimental autoimmune encephalomyelitis is mediated by SOCS3 regulatory pathway

Cited by 73 publications

References 50 publications

Treatment with 6‐Gingerol Regulates Dendritic Cell Activity and Ameliorates the Severity of Experimental Autoimmune Encephalomyelitis

Treatment with 6‐Gingerol Regulates Dendritic Cell Activity and Ameliorates the Severity of Experimental Autoimmune Encephalomyelitis

FSD-C10, a Fasudil derivative, promotes neuroregeneration through indirect and direct mechanisms

Western lifestyle and immunopathology of multiple sclerosis

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