Therapeutic effect of Cnidium officinale Makino extract on ovariectomized hind-limb ischemic mice

Abstract

Background Cnidium officinale Makino (COM) has been used traditionally to treat female menstrual disorders, such as amenorrhea, hypomenorrhea and oligomenorrhea, by improving blood circulation. The present study aimed to investigate the alleviating effect of COM extracts on surgical injuryinduced ischemia in the hind-limb of mice. Methods In this study, female C57BL/6 mice were ovariectomized, and the vessels of the hind-limb were excised after ligation by surgical silk (6–0). The mice were orally administered with COM (150 or 300 mg/kg/day) for 3 weeks, and the blood flow rate of hind-limbs was evaluated by using a laser Doppler system after hind-limb ischemic surgery in an in vivo study. Additionally, the migration and tube formation of human umbilical vein endothelial cells (HUVECs) were evaluated in an in vitro study. Results The blood flow rate was synchronized to the nonischemic lesion of the hind-limb, and its elevation compared to the vehicle was observed at 14 and 21 days after hind-limb ischemic surgery in COM-treated groups. The number of capillaries increase in a dose-dependent manner in the COM-treated groups (150 and 300 mg/kg). In HUVECs, the activities of cell migration were significantly increased by 50 and 75 μg/mL for the COM-treated groups. In addition, the number of tubule branches and junctions was also increased by doses of COM (50 and/or 75 μg/mL). Conclusion The results of our study suggested that the COM extract may have therapeutic application for the treatment of hind-limb ischemic damage, which is due to the improvement of the peripheral angiogenetic system.

A review of frequently used Kampo prescriptions. Part 3. Yokukansan

“…Cnidium rhizome has multiple pharmacological activities, including anti‐inflammatory [26], antioxidant [27], antimicrobial [28], antifungal [29], acaricidal [30], antitumor [31], and analgesic [32] activities. It also exerts vascular [33], nerve [34], and renal protective effects [35].…”

Cnidium officinale Makino: Phytology, Phytochemistry, Toxicology, Pharmacology and Prescriptions (1967–2023)

“…[52] In hind-limb ischemic mouse model COM extract improves the peripheral angiogenetic system. [53] Similarly, distilled water extracts of COM and butylidenephthalide has anti-angiogenic activities on retinal neovascularization by inhibiting the expression of AREG, ANG, DLL4 and VEGF. [54] As shown in Figure 14.…”

Genetic diversity of viruses infecting cnidium plants (Cnidium officinale) in Japan: cnidium vein yellowing virus (CnVYV), cnidium virus X (CnVX) and cucumber mosaic virus (CMV)

“…This plant was introduced in Japan in mid 1600s, rst cultivated in Hokkaido in the middle of the 1800s, and is mainly grown in Hokkaido and Iwate prefectures at present. C. o cinale is vegetatively propagated using divided rhizome as a seed tuber for cultivation and its rhizome has medicinal use in the treatment of anemia, pyogenic skin diseases, and gynecological disorders in China, Korea, Japan, and other Asian countries [1,2]. Many viral infections, such as cnidium vein yellowing virus (CnVYV), cnidiun virus X (CnVX) and cucumber mosaic virus (CMV) infections, have been described in this plant in China, Korea, and Japan [3,4,5,6].…”