Therapeutic effect of icariin combined with stem cells on postmenopausal osteoporosis in rats

Tang, Daolin; Ju, Cuiling; Liu, Yanjie; Xu, Fei; Wang, Zhengguang; Wang, Dongbo

doi:10.1007/s00774-017-0831-x

Cited by 22 publications

(29 citation statements)

References 25 publications

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“…Icariin is a major flavonoid in Herba Epimedii, which has an osteogenic effect [18][19][20]. Studies have reported that icariin could increase the proliferation and differentiation of pre-osteoblasts, osteoblasts and rat bone marrow stromal cells [21,22].…”

Section: Discussionmentioning

confidence: 99%

Icariin induces MC3T3-E1 cell proliferation and differentiation via the BMP-2/Smads/Runx2 signal pathway

Gou

et al. 2018

Preprint

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Icariin, the main active ingredient of Epimedium, has played an important role in bone anabolism. However, the molecular mechanism for this effect was not convincingly reported yet. In this paper, the role of icariin on cell morphology, viability, cell cycling and the activity of alkaline phosphatase (ALP) were studied, and the molecular mechanism of icariin induced osteogenic effect was also investigated. Icariin with different concentrations (10, 20 and 40 ng/ml) was used to modify the pre-osteoblastic MC3T3-E1 cells for 48, 72 and 96 h, and the proliferation, morphology, and the cell cycle of the cells were evaluated by Cell Counting Kit-8 (CCK-8), microscopy and flow cytometry, respectively. Bone morphogenic protein-2 (BMP-2), bone morphogenic protein receptor-2 (BMPR-2), Smad4, Smadl/5/8 proteins expression levels were obtained by Western blotting and the expression levels of runt-related transcription factor 2 (Runx2) mRNA was examined by reverse transcription-polymerase chain reaction (RT-PCR). In this study, we found that icariin could promote the proliferation and differentiation of MC3T3-E1 cells in a dose-and time-dependent manner. Icariin could stimulate the expression of the BMP-2, BMPR-2, Smad4 and Smadl/5/8 proteins.Furthermore, icariin could upregulate the expression of Runx2 mRNA. These results showed that icariin played an important role in upregulating BMP-2 expression to activate the BMP-2/Smads/Runx2 signal pathway for increasing both the proliferation and differentiation of the MC3T3-E1 cells. However, the osteogenic effects of icariin can be suppressed by the BMP-2 antagonist (Noggin). In conclusion, we demonstrate that icariin is an osteoinductive factor that exerts its osteogenic effect by regulating the BMP-2/Smads/Runx2 signal pathway in MC3T3-E1 cells.

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Section: Discussionmentioning

confidence: 99%

Icariin induces MC3T3-E1 cell proliferation and differentiation via the BMP-2/Smads/Runx2 signal pathway

Gou

et al. 2018

Preprint

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“…ADSCs cells were isolated as described in a previous study. 16 Grouping A total of 50 rats were randomly selected for bilateral ovarian ablation. Rats were divided into 4 groups randomly (n=9): the model group, the icariin group (250 mg/kg/d, intragastric administration), ADSCs group (ADSCs 2×10 5 cell/ml, intramedullary injection, 0.5ml each time), icariin combined with ADSCs group (icariin + ADSCs group, icariin 250 mg/kg/d, intragastric administration; ADSCs 2×10 5 cell/ml, intramedullary injection, 0.…”

Section: Pmop Rat Modelmentioning

confidence: 99%

“…A previous study have shown that icariin combined with ADSCs can improve bone mass reduction and bone microstructural destruction in osteoporosis rat model, they have protective effects on liver function and kidney. 16 However, the mechanism has not been explored. Therefore, as a further study, we investigated the expression of CALCR and CTSK protein, and the ratio of OPG / RANKL in femur tissue, to explore the mechanism of icariin combined with ADSCs on PMOP rats, and to provide a new theoretical basis for osteoporosis clinical treatment.…”

Section: Introductionmentioning

confidence: 99%

Icariin Combined with Adipose-Derived Stem Cells Ameliorated Osteoporosis in Postmenopausal Rats

Sun¹,

Wang²,

Tang³

et al. 2020

Preprint

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Purpose: Osteoporosis is a chronic metabolic disease, it has caused the high incidence of related fractures, which seriously affects the life quality of the patients, especially in early postmenopausal women. Methods: Rat osteoporosis model was induced by bilateral ovarian ablation. Sprague Dawley (SD) rats were randomly divided into five groups (n=9): sham group, model group, the icariin group, ADSCs group, icariin combined with ADSCs group. H&E staining was used to observe the pathological changes of femur. The expression of calcitonin receptor (CALCR) and cathepsin K (CTSK) were investigated by immunohistochemistry. The expression of osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) were measured by qRT-PCR, immunohistochemistry and western blot.Results: The results showed that icariin combined with ADSCs ameliorated osteoblast morphology and bone density. Icariin combined with ADSCs significantly attenuated the protein expression of CALCR and CTSK after ovariectomy. Moreover, the expressions of OPG mRNA and protein were increased (p < 0.05), while the expressions of RANKL mRNA and protein were decreased (p < 0.05) in osteoblasts.Conclusions: Icariin combined with ADSCs can effectively inhibit PMOP in rats. The mechanism may be achieved by up-regulated the ratio of OPG / RANKL.

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“…Further exploration revealed that mechanism involved upregulation of peak bone mass, increased mineralization, and induction of growth maturation of osteoblasts. A study exploring synergism between Icariin and Adipose- derived stem cell transplantation in osteoporotic rats illustrated that the combination enhanced the serum levels of TRAP, ALP activity, OPG and bone γ-carboxyglutamate protein ( Tang et al, 2018 ). Further, the treatment was found to significantly reduce body weight gain and elevate BMD of lumbar spine, femur bone, in addition to improvement in biomechanical properties of femur.…”

Section: Icariinmentioning

confidence: 99%

Bone Health and Natural Products- An Insight

et al. 2018

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Bone metabolism involves a complex balance between matrix deposition, mineralization, and resorption. Numerous evidences have revealed that dietary components and phytoconstituents can influence these processes, through inhibition of bone resorption, thus exhibiting beneficial effects on the skeleton. Various traditional herbal formulae in ayurvedic and Chinese medicine have shown demonstrable benefits in pharmacological models of osteoporosis. The present review discusses normal bone metabolism and disorders caused by bone disruption, with particular reference to osteoporosis and current therapeutic treatment. Furthermore the effects of constituents from natural products on bone tissue are explained, with relevant evidences of efficacy in various experimental models.

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Therapeutic effect of icariin combined with stem cells on postmenopausal osteoporosis in rats

Cited by 22 publications

References 25 publications

Icariin induces MC3T3-E1 cell proliferation and differentiation via the BMP-2/Smads/Runx2 signal pathway

Icariin induces MC3T3-E1 cell proliferation and differentiation via the BMP-2/Smads/Runx2 signal pathway

Icariin Combined with Adipose-Derived Stem Cells Ameliorated Osteoporosis in Postmenopausal Rats

Bone Health and Natural Products- An Insight

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