Therapeutic Effects of Bilateral Anodal Transcranial Direct Current Stimulation on Prefrontal and Motor Cortical Areas in Children with Autism Spectrum Disorders: A Pilot Study

Hadoush, Hikmat; Nazzal, Mohammad; Almasri, Nihad A.; Khalil, Hanan; Alafeef, Maha

doi:10.1002/aur.2290

Cited by 40 publications

(50 citation statements)

References 42 publications

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“…• Gel quantification software (Bio-Rad ImageLab) • PrimerExplorerV5 (https://primerexplorer.jp/e/) • Sensitivity, specificity and accuracy has been calculated using MedCalc Free statistical calculators (https://www.medcalc.org/calc/diagnostic_test.php) based on the following equations: [75][76][77][78][79][80] Accuracy is the total number of samples correctly diagnosed over the total number of tested samples. The accuracy can be calculated using the following equation:…”

Section: Softwarementioning

confidence: 99%

RNA-extraction-free nano-amplified colorimetric test for point-of-care clinical diagnosis of COVID-19

et al. 2021

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The global pandemic of coronavirus disease 2019 highlights the shortcomings of the current testing paradigm for viral disease diagnostics. Here, we report a stepwise protocol for an RNA-extraction-free nano-amplified colorimetric test for rapid and naked-eye molecular diagnosis of COVID-19. The test employs a unique dual-prong approach that integrates nucleic acid (NA) amplification and plasmonic sensing for point-of-care detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), with a sample-to-assay response time of <1 h. The RNA-extraction-free nanoamplified colorimetric test utilizes plasmonic gold nanoparticles capped with antisense oligonucleotides (ASOs) as a colorimetric reporter to detect the amplified nucleic acid from the COVID-19 causative virus, SARS-CoV-2. The ASOs are specific for the SARS-CoV-2 N-gene, and binding of the ASOs to their target sequence results in the aggregation of the plasmonic gold nanoparticles. This highly specific agglomeration step leads to a change in the plasmonic response of the nanoparticles. Furthermore, when tested using clinical samples, the accuracy, sensitivity and specificity of the test were found to be >98.4%, >96.6% and 100%, respectively, with a detection limit of 10 copies/μL. The test can easily be adapted to diagnose other viral infections with a simple modification of the ASOs and primer sequences. It also provides a low-cost, rapid approach requiring minimal instrumentation that can be used as a screening tool for the diagnosis of COVID-19 at point-of-care settings in resource-poor situations. The colorimetric readout of the test can even be monitored using a handheld optical reader to obtain a quantitative response. Therefore, we anticipate that this protocol will be widely useful for the development of biosensors for the molecular diagnostics of COVID-19 and other infectious diseases.

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Section: Softwarementioning

confidence: 99%

RNA-extraction-free nano-amplified colorimetric test for point-of-care clinical diagnosis of COVID-19

et al. 2021

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“…The Autism Treatment Evaluation Checklist [ATEC, (28)] was used to measure the efficacy of PCBI. It has been successfully used to assess the effectiveness of treatments and progress after intervention in ASD (29)(30)(31). The ATEC is designed to be self-administered by parents and elicits parent concerns in four domains: speech/language/communication (14 items, scores range from 0 to 28), health/physical behavior (25 items, scores range from 0 to 75), sensory/cognitive awareness (18 items, scores range from 0 to 36), and sociability (20 items, scores range from 0 to 40) (32).…”

Section: Efficacy Assessmentmentioning

confidence: 99%

Predictors of Change in Play-Based Communication and Behavior Intervention for High-Risk ASD: The Role of Mother-Child Dyadic Synchrony

Weng

Feng

et al. 2020

Front. Pediatr.

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Background: Interindividual variability is important in the evolution of adaptative profiles of children with ASD having benefited from an early intervention make up for deficits in communication, language and social interactions. Therefore, this paper aimed to determine the nature of factors influencing the efficacy variability of a particular intervention technique i.e., “Play-based communication and behavior intervention” (PCBI).Methods: The participants comprised 70 13–30-month-old toddlers with ASD enrolled in PCBI for 12 weeks. The Autism Treatment Evaluation Checklist (ATEC) was used to evaluate the efficacy of PCBI. Video recordings of 5 min of free-play before and after PCBI were used to examine behaviors of mothers and children and parent-child dyadic synchrony. Hierarchical multiple regression analyses and machine learning algorithms were performed to explore the effect of these potential predictors (mothers' factors, children's factors and videotaped mother-child interaction) of intervention efficacy.Results: The hierarchical regression analysis and the machine learning algorithms indicated that parenting stress, level of completion of training at home and mother-child dyadic synchrony were crucial factors in predicting and monitoring the efficacy of PCBI.Conclusions: In summary, the findings suggest that PCBI could be particularly beneficial to children with ASD who show a good performance in the mother-child dyadic synchrony evaluation. A better dyadic mother-child synchrony could enhance the PCBI efficacy through adapted emotional and behavioral responses of the mother and the child and has a beneficial influence on the child's psychological development.

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“…Most recently, Hadoush et al [ 42 ] studied the potential therapeutic effects of tDCS on the clinical characteristics of children with ASD. The tDCS treatment group underwent 10 sessions (1 mA, 20 min durations, five per week) of bilateral anodal tDCS stimulation applied simultaneously over the left and right prefrontal and motor areas (FC1 and FC2 of EEG system) and the cathode electrodes applied over the left and right supraorbital areas.…”

Section: Introductionmentioning

confidence: 99%

Transcranial Direct Current Stimulation (tDCS) over the Left Dorsal Lateral Prefrontal Cortex in Children with Autism Spectrum Disorder (ASD)

Qiu

Kong

et al. 2021

Neural Plasticity

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Recently, transcranial direct current stimulation (tDCS) has been applied to relieve symptoms in individuals with autism spectrum disorder (ASD). In this prospective, parallel, single-blinded, randomized study, we investigate the modulation effect of three-week tDCS treatment at the left dorsal lateral prefrontal cortex (DLPFC) in children with ASD. 47 children with ASD were enrolled, and 40 (20 in each group) completed the study. The primary outcomes are Childhood Autism Rating Scale (CARS), Aberrant Behavior Checklist (ABC), and the Repetitive Behavior Scale-Revised (RBS-R). We found that children with ASD can tolerate three-week tDCS treatment with no serious adverse events detected. A within-group comparison showed that real tDCS, but not sham tDCS, can significantly reduce the scores of CARS, Children’s Sleep Habits Questionnaire (CSHQ), and general impressions in CARS (15th item). Real tDCS produced significant score reduction in the CSHQ and in CARS general impressions when compared to the effects of sham tDCS. The pilot study suggests that three-week left DLPFC tDCS is well-tolerated and may hold potential in relieving some symptoms in children with ASD.

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Therapeutic Effects of Bilateral Anodal Transcranial Direct Current Stimulation on Prefrontal and Motor Cortical Areas in Children with Autism Spectrum Disorders: A Pilot Study

Cited by 40 publications

References 42 publications

RNA-extraction-free nano-amplified colorimetric test for point-of-care clinical diagnosis of COVID-19

RNA-extraction-free nano-amplified colorimetric test for point-of-care clinical diagnosis of COVID-19

Predictors of Change in Play-Based Communication and Behavior Intervention for High-Risk ASD: The Role of Mother-Child Dyadic Synchrony

Transcranial Direct Current Stimulation (tDCS) over the Left Dorsal Lateral Prefrontal Cortex in Children with Autism Spectrum Disorder (ASD)

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