Therapeutic Effects of Human Adipose-Derived Products on Impaired Wound Healing in Irradiated Tissue

Wu, Szu-Hsien; Shirado, Takako; Mashiko, Takafumi; Feng, Jiaxuan; Asahi, Rintaro; Kanayama, Koji; Mori, Masanori; Chi, Daekwan; Sunaga, Ataru; Sarukawa, Shunji; Yoshimura, Kotaro

doi:10.1097/prs.0000000000004609

Cited by 32 publications

(47 citation statements)

References 35 publications

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“…In conclusion, ADSC transplantation accelerated LEC proliferation, increased lymphatic vessel numbers, and mitigated fibrosis of the surrounding interstitial tissue. ADSC transplantation efficacy on wound healing is established [24,59,60]. For clinical secondary lymphedema and lymphorrhea, which are thought to be caused by surgical lymph node dissection and radiotherapy, ADSC transplantation is considered to be effective for both lymphatic function recovery and wound healing.…”

Section: Discussionmentioning

confidence: 99%

“…Growth factors secreted by ADSC transplantation (i.e., VEGF, FGF2, TGF-β, HGF, platelet-derived growth factor (PDGF), keratinocyte growth factor (KGF), fibronectin, and collagen I and increased expression of VEGFR-3) promote lymphatic regeneration [24,26,59,61]. Factors associated with lymphangiogenesis and fibrosis include: Tgfb1, suppression of which improves both fibrosis and lymphangiogenesis [18,62]; tumor necrosis factor (TNF)-α and interleukin (IL)-1 [48]; and neutrophils that increase lymphatic vessel density by increasing the number of VEGF-A/VEGFR-2 complexes and releasing VEGF-D [14].…”

Section: Discussionmentioning

confidence: 99%

“…ADSCs are radioresistant, continue to release growth factors in vitro [23], and have the ability of wound healing in irradiated tissue [24,25]. They exert antiwrinkle effects by reducing ultraviolet-B-induced apoptosis, stimulating collagen synthesis of dermal fibrosis [26], and modifying the local microenvironment [27].…”

Section: Introductionmentioning

confidence: 99%

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Adipose-Derived Stem Cells Promote Intussusceptive Lymphangiogenesis by Restricting Dermal Fibrosis in Irradiated Tissue of Mice

Ogino

Hayashida

Yamakawa

et al. 2020

IJMS

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Currently, there is no definitive treatment for lymphatic disorders. Adipose-derived stem cells (ADSCs) have been reported to promote lymphatic regeneration in lymphedema models, but the mechanisms underlying the therapeutic effects remain unclear. Here, we tested the therapeutic effects of ADSC transplantation on lymphedema using a secondary lymphedema mouse model. The model was established in C57BL/6J mice by x-irradiation and surgical removal of the lymphatic system in situ. The number of lymphatic vessels with anti-lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) immunoreactivity increased significantly in mice subjected to transplantation of 7.5 × 105 ADSCs. X-irradiation suppressed lymphatic vessel dilation, which ADSC transplantation could mitigate. Proliferative cell nuclear antigen staining showed increased lymphatic endothelial cell (LEC) and extracellular matrix proliferation. Picrosirius red staining revealed normal collagen fiber orientation in the dermal tissue after ADSC transplantation. These therapeutic effects were not related to vascular endothelial growth factor (VEGF)-C expression. Scanning electron microscopy revealed structures similar to the intraluminal pillar during intussusceptive angiogenesis on the inside of dilated lymphatic vessels. We predicted that intussusceptive lymphangiogenesis occurred in lymphedema. Our findings indicate that ADSC transplantation contributes to lymphedema reduction by promoting LEC proliferation, improving fibrosis and dilation capacity of lymphatic vessels, and increasing the number of lymphatic vessels via intussusceptive lymphangiogenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Adipose-Derived Stem Cells Promote Intussusceptive Lymphangiogenesis by Restricting Dermal Fibrosis in Irradiated Tissue of Mice

Ogino

Hayashida

Yamakawa

et al. 2020

IJMS

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“…Since the discovery and characterization of the multipotent stem cell population in adipose tissue by Patricia Zuk et al in 2002 [ 5 ], the regenerative properties of the stromal vascular fraction (SVF) have been investigated in multiple preclinical and clinical models [ 6 , 7 , 8 , 9 , 10 ]. The SVF is a cell population that can be conjointly retrieved by the process of enzymatic dissociation filtration and centrifugation of adipose tissue [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Treatment of Grade 3 and 4 Osteoarthritis with Intraoperatively Separated Adipose Tissue-Derived Stromal Vascular Fraction: A Comparative Case Series

Simunec

Salari

Meyer

2020

Cells

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Osteoarthritis (OA) is the most common form of arthritis of the joints. The stromal vascular fraction (SVF) is a regenerative cell population that can be isolated from adipose tissue. It is the immunomodulatory properties of the stromal vascular fraction that make it a promising candidate for the regenerative treatment of OA. Patients with grade 3 and 4 osteoarthritis were treated with the stromal vascular fraction with and without platelet-rich plasma (PRP) and followed up on their Knee Injury and Osteoarthritis Outcome Score (KOOS) score for 12 months, with MRI and subjective evaluation of the procedure. Magnetic resonance imaging (MRI) revealed a widening of the joint space, a restructuring of the cartilage, and an alleviation of effusions in the treated joints. In three of the four treatment groups, a substantial improvement of the KOOS scores was documented at the 12-month follow-up time point. According to the subjective evaluation, 67% of the patients were satisfied or very satisfied with the procedure and would recommend it to others. No serious adverse events or unwanted side effects related to the SVF treatment were observed or reported. Prior to an invasive artificial joint replacement, the treatment of arthritic knee joints with the intraarticular injection of autologous adipose tissue-derived SVF should be considered a regenerative treatment option.

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“…The treatment of GVHD has been the most widely investigated with BM-MSCs after allogeneic HSCs transplantation and or lymphocyte infusion (Table 1). In adult patients and children with steroid-resistant acute GVHD, the infusion of allogeneic BM-MSCs resulted in the complete response and overall survival in most patients after UCB and HSCs transplantation without any side-effects during or immediately after MSCs infusion [167,168], while better outcomes were also observed with early BM-MSCs administration [169] (Figure 3). In malignancies, BM-MSCs were safe and successful in improving hematopoietic engraftment [170,197], and similar results have been reported with UCB-MSCs in pediatric and adult patients after HSC transplantation [175,176].…”

Section: Current Approaches In Adipose Derived Stem Cells (Adscs)-mentioning

confidence: 99%

Regenerative Capacity of Adipose Derived Stem Cells (ADSCs), Comparison with Mesenchymal Stem Cells (MSCs)

Mazini

Rochette

Amine

et al. 2019

IJMS

314

212

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Adipose tissue is now on the top one of stem cell sources regarding its accessibility, abundance, and less painful collection procedure when compared to other sources. The adipose derived stem cells (ADSCs) that it contains can be maintained and expanded in culture for long periods of time without losing their differentiation capacity, leading to large cell quantities being increasingly used in cell therapy purposes. Many reports showed that ADSCs-based cell therapy products demonstrated optimal efficacy and efficiency in some clinical indications for both autologous and allogeneic purposes, hence becoming considered as potential tools for replacing, repairing, and regenerating dead or damaged cells. In this review, we analyzed the therapeutic advancement of ADSCs in comparison to bone marrow (BM) and umbilical cord (UC)-mesenchymal stem cells (MSCs) and designed the specific requirements to their best clinical practices and safety. Our analysis was focused on the ADSCs, rather than the whole stromal vascular fraction (SVF) cell populations, to facilitate characterization that is related to their source of origins. Clinical outcomes improvement suggested that these cells hold great promise in stem cell-based therapies in neurodegenerative, cardiovascular, and auto-immunes diseases.

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Therapeutic Effects of Human Adipose-Derived Products on Impaired Wound Healing in Irradiated Tissue

Abstract: Tissue devitalization by irradiation was dose-dependent, although fractionated protocols helped to reduce it. Adipose-derived stem cells and other fat-derived products harboring adipose-derived stem cells successfully revitalized irradiated tissues and accelerated wound healing.

Cited by 32 publications

References 35 publications

Adipose-Derived Stem Cells Promote Intussusceptive Lymphangiogenesis by Restricting Dermal Fibrosis in Irradiated Tissue of Mice

Adipose-Derived Stem Cells Promote Intussusceptive Lymphangiogenesis by Restricting Dermal Fibrosis in Irradiated Tissue of Mice

Treatment of Grade 3 and 4 Osteoarthritis with Intraoperatively Separated Adipose Tissue-Derived Stromal Vascular Fraction: A Comparative Case Series

Regenerative Capacity of Adipose Derived Stem Cells (ADSCs), Comparison with Mesenchymal Stem Cells (MSCs)

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