Therapeutic Effects of Milk Thistle Extract against Renal Toxicity Induced By Diethylnitrosamine and Carbon Tetrachloride in Adult Rats

Ahmed, Amira; Hassanin, Abeer A. I.; Hassan, Abeer S.; Ali, Saadia Sasha; El-Anwar, A.

doi:10.21608/jva.2017.37166

Cited by 3 publications

(1 citation statement)

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“…These results indicated a profound activation of HSCs in response to DEN+CCl4 intoxication. Similarly, previous studies showed that α-SMA expression as well as collagen and hydroxyproline content were significantly elevated in response to injection with CCl4 alone or in combination with DEN ( Mansour et al, 2010 ; Abdel-Moneim et al, 2015 ; Ahmed and Tag, 2017 ).…”

Section: Discussionsupporting

confidence: 71%

Antisense Tissue Factor Oligodeoxynucleotides Protected Diethyl Nitrosamine/Carbon Tetrachloride-Induced Liver Fibrosis Through Toll Like Receptor4-Tissue Factor-Protease Activated Receptor1 Pathway

et al. 2021

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Tissue factor (TF) is a blood coagulation factor that has several roles in many non-coagulant pathways involved in different pathological conditions such as angiogenesis, inflammation and fibrogenesis. Coagulation and inflammation are crosslinked with liver fibrosis where protease-activated receptor1 (PAR1) and toll-like receptor4 (TLR4) play a key role. Antisense oligodeoxynucleotides are strong modulators of gene expression. In the present study, antisense TF oligodeoxynucleotides (TFAS) was evaluated in treating liver fibrosis via suppression of TF gene expression. Liver fibrosis was induced in rats by a single administration of N-diethyl nitrosamine (DEN, 200 mg/kg; i. p.) followed by carbon tetrachloride (CCl4, 3 ml/kg; s. c.) once weekly for 6 weeks. Following fibrosis induction, liver TF expression was significantly upregulated along with liver enzymes activities and liver histopathological deterioration. Alpha smooth muscle actin (α-SMA) and transforming growth factor-1beta (TGF-1β) expression, tumor necrosis factor-alpha (TNF-α) and hydroxyproline content and collagen deposition were significantly elevated in the liver. Blocking of TF expression by TFAS injection (2.8 mg/kg; s. c.) once weekly for 6 weeks significantly restored liver enzymes activities and improved histopathological features along with decreasing the elevated α-SMA, TGF-1β, TNF-α, hydroxyproline and collagen. Moreover, TFAS decreased the expression of both PAR1 and TLR4 that were induced by liver fibrosis. In conclusion, we reported that blockage of TF expression by TFAS improved inflammatory and fibrotic changes associated with CCl4+DEN intoxication. In addition, we explored the potential crosslink between the TF, PAR1 and TLR4 in liver fibrogenesis. These findings offer a platform on which recovery from liver fibrosis could be mediated through targeting TF expression.

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Section: Discussionsupporting

confidence: 71%

Antisense Tissue Factor Oligodeoxynucleotides Protected Diethyl Nitrosamine/Carbon Tetrachloride-Induced Liver Fibrosis Through Toll Like Receptor4-Tissue Factor-Protease Activated Receptor1 Pathway

et al. 2021

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Bone marrow-derived mesenchymal stem cells reduce CCl ₄ -induced kidney injury and fibrosis in male Wistar rats

Adel,

Abdul-Hamid,

Abdel-Kawi

et al. 2024

Renal Failure

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Chemopreventive Efficacy of <i>Punica granatum</i> and <i>Silybum marianum </i>Extracts on Chemically-induced Hepatocellular Carcinoma in Rats

Tag

Bargougui²,

Alshayyal³

et al. 2019

AJAS

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Punica granatum (POM) and Silybum marianum (MT) receiving attention as potential potent anti-oxidant and anti-mutant agents. In this context, the present study was designed to highlight their effects either in vitro as well as in vivo model of induced Hepatocellular carcinoma (HCC). Human hepatoma (HepG2 cells) were treated with MT and POM to explore their antitumor activity then in vivo were carried out on thirty-six male albino rats divided into six groups (n=6). Two weeks after induction of HCC, rats were co-treated with either MT or POM ethanolic extract (500 mg/kg, orally) daily for 8 weeks. The results displayed marked reduction in the viability of HepG2 cells with IC50 equal to 48.4 and 8.6 Î¼g/mL of POM and MT treatment respectively. Considering, in vivo experiment HCC group displayed significant elevation liver function indices (p<0.05). It also elicited depletion of liver reduced glutathione (GSH), and increased content of liver malondialdehyde (MDA) compared to control group. HCC was proved after a significantly elevated alpha-fetoprotein (AFP) level (p<0.05). All of these measurements were diminished significantly after POM and MT treatments, except the GSH level that was increased significantly. Supplementation of pomegranate and milk thistle extracts had a protective effect against chemically induced HCC.Â

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Therapeutic Effects of Milk Thistle Extract against Renal Toxicity Induced By Diethylnitrosamine and Carbon Tetrachloride in Adult Rats

Abstract: The rapeutic effects of milk agains t renal toxicity in rats Ahmed and Tag

Cited by 3 publications

References 1 publication

Antisense Tissue Factor Oligodeoxynucleotides Protected Diethyl Nitrosamine/Carbon Tetrachloride-Induced Liver Fibrosis Through Toll Like Receptor4-Tissue Factor-Protease Activated Receptor1 Pathway

Antisense Tissue Factor Oligodeoxynucleotides Protected Diethyl Nitrosamine/Carbon Tetrachloride-Induced Liver Fibrosis Through Toll Like Receptor4-Tissue Factor-Protease Activated Receptor1 Pathway

Bone marrow-derived mesenchymal stem cells reduce CCl ₄ -induced kidney injury and fibrosis in male Wistar rats

Chemopreventive Efficacy of <i>Punica granatum</i> and <i>Silybum marianum </i>Extracts on Chemically-induced Hepatocellular Carcinoma in Rats

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Therapeutic Effects of Milk Thistle Extract against Renal Toxicity Induced By Diethylnitrosamine and Carbon Tetrachloride in Adult Rats

Abstract: The rapeutic effects of milk agains t renal toxicity in rats Ahmed and Tag

Cited by 3 publications

References 1 publication

Antisense Tissue Factor Oligodeoxynucleotides Protected Diethyl Nitrosamine/Carbon Tetrachloride-Induced Liver Fibrosis Through Toll Like Receptor4-Tissue Factor-Protease Activated Receptor1 Pathway

Antisense Tissue Factor Oligodeoxynucleotides Protected Diethyl Nitrosamine/Carbon Tetrachloride-Induced Liver Fibrosis Through Toll Like Receptor4-Tissue Factor-Protease Activated Receptor1 Pathway

Bone marrow-derived mesenchymal stem cells reduce CCl 4 -induced kidney injury and fibrosis in male Wistar rats

Chemopreventive Efficacy of <i>Punica granatum</i> and <i>Silybum marianum </i>Extracts on Chemically-induced Hepatocellular Carcinoma in Rats

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Bone marrow-derived mesenchymal stem cells reduce CCl ₄ -induced kidney injury and fibrosis in male Wistar rats