Therapeutic effects of PG201, an ethanol extract from herbs, through cartilage protection on collagenase-induced arthritis in rabbits

Park, Kyoung Chul; Park, Eun Jin; Kim, Eun Ran; Kim, Yeon-Ran; Chung, Seung Hyun; Cho, Byoung Wook; Kim, Sun‐Young; Jin, Myung

doi:10.1016/j.bbrc.2005.04.030

Cited by 18 publications

(16 citation statements)

References 37 publications

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“…NC, negative control; PGE 2 , prostaglandin E 2 ; cPLA 2 , cytosolic phospholipase A 2 ; LPS, lipopolysaccharide; ELISA, enzyme-linked immunosorbent assay; COX-2, cyclooxygenase-2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; CHX, cycloheximide; DMEM, Dulbecco's modified Eagle's medium; PBS, phosphate-buffered saline; BSA, bovine serum albumin decreased the level of IL-1b, while simultaneously preventing the degradation of proteoglycan in the collagenase-induced OA rabbit model. 19 Data from the Northern blot and gel retardation analyses showed that PG201 decreased the expressions of IL-1b, IL-6 and CCL2 at the RNA level, probably by regulating AP-1 and CREB transcription factors. 11,12,14 AP-1 is an important factor for the initiation of chronic inflammatory diseases, such as RA, inflammatory bowel disease and psoriasis, and it controls the expression of many genes involved in RA including MMPs.…”

Section: Discussionmentioning

confidence: 98%

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Suppressive effects of PG201, an antiarthritic botanical formulation, on lipopolysaccharide-induced inflammatory mediators in Raw264.7 cells

Choi

Kim²,

Kim

2012

Exp Biol Med (Maywood)

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PG201, an ethanol extract from a mixture of 12 herbs, has strong antiarthritic activity. To understand the molecular mechanisms underlying its anti-inflammatory effects, PG201-mediated suppression of inflammatory mediators was studied in Raw264.7, a mouse macrophage cell line. PG201 decreased the expression of interleukin (IL)-1β, IL-6 and CC chemokine ligand-2, but not tumor necrosis factor-α, at the protein and mRNA levels in lipopolysaccharide-stimulated Raw264.7 cells. Results from a gel retardation assay indicated that PG201 substantially reduced the DNA-binding activity of the activator protein-1 and cyclic adenosine monophosphate-responsive element-binding protein transcription factors, but not nuclear factor-κB. Western blot and Northern blot analyses showed that PG201 reduced inducible nitric oxide synthase and cytosolic phospholipase A(2) (cPLA(2)) protein expression, but did not affect mRNA expression, ultimately resulting in decreased nitric oxide and prostaglandin E(2). The protein expression of cPLA(2) was decreased by PG201 in the presence of cycloheximide, an inhibitor of translation, suggesting that PG201 may facilitate the degradation of cPLA(2). Taken together, these results suggest that PG201 selectively affects the expression of proteins that play key roles in the inflammatory response at transcriptional and post-translational levels.

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Section: Discussionmentioning

confidence: 98%

“…18,19 PG201 inhibited the expression of TNF-a and IL-1b in a RA mouse model. In an OA rabbit model, PG201 down-regulated the expression of IL-1b, NO and PGE 2 , as well as MMPs.…”

Section: Introductionmentioning

confidence: 99%

Suppressive effects of PG201, an antiarthritic botanical formulation, on lipopolysaccharide-induced inflammatory mediators in Raw264.7 cells

Choi

Kim²,

Kim

2012

Exp Biol Med (Maywood)

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“…Matrix metalloproteinases (MMPs), which contribute to the accelerated degradation of extracellular matrix and tissue inhibitors of metalloproteinases (TIMPs) that regulates the MMP activity, play an important function in cartilage matrix turnover [8]. However, in OA affected joint tissues, up-regulation of MMPs is reported higher compared to TIMPs [8]. Altogether, these observations support the concept of antioxidant therapy along with modulation of MMP-TIMP might also decrease the progression of OA.…”

Section: Introductionmentioning

confidence: 95%

“…To prevent toxicity by ROS, chondrocytes express a well co-ordinated anti-oxidant enzyme system in the form of SOD, CAT and GPX [6]. Matrix metalloproteinases (MMPs), which contribute to the accelerated degradation of extracellular matrix and tissue inhibitors of metalloproteinases (TIMPs) that regulates the MMP activity, play an important function in cartilage matrix turnover [8]. However, in OA affected joint tissues, up-regulation of MMPs is reported higher compared to TIMPs [8].…”

Section: Introductionmentioning

confidence: 99%

New herbal composition (OA-F2) protects cartilage degeneration in a rat model of collagenase induced osteoarthritis

Nirmal

Jagtap

Narkhede

et al. 2017

BMC Complement Altern Med

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BackgroundPrevalence of osteoarthritis (OA) is on rise on the global scale. At present there are no satisfactory pharmacological agents for treating OA. Our previous study showed that Sida cordifolia L. and Zingiber officinale Rosc. had protective effect on cartilage. Here, we describe the effect of OA-F2, a herbal formulation prepared using combination of these two plants in alleviating OA associated symptoms in a rat model of collagenase-induced OA.MethodsOA was induced by intra-articular injection of collagenase type II in wistar rats. Diclofenac (10 mg/kg) was used as a reference control. Rats (n = 6) were divided into 6 groups: Healthy control (HC), osteoarthritic control (OAC), diclofenac (DICLO), OA-F2L (135 mg/kg), OA-F2M (270 mg/kg) and OA-F2H (540 mg/kg). The effects of the 20 days treatment were monitored by parameters like knee diameter, paw volume, paw retraction; serum C-reactive protein (CRP), alkaline phosphatase (ALP) and glycosaminoglycan (GAG). Radiography and histopathology of knee joint were also studied. Additionally, gene expression was studied from isolated synovium tissue proving anti-osteoarthritic potential of OA-F2.ResultsOral administration of OA-F2 has significantly prevented knee swelling compared to OAC; OA-F2 and DICLO, significantly reduced paw volume compared to OAC. Paw latency was remarkably increased by OA-F2 compared to OAC. OA-F2L (−0.670, p < 0.001), M (−0.110, p < 0.05) and H (0.073) has markedly reduced levels of CRP compared to DICLO. OA-F2L (p < 0.05), M (p < 0.001) and H (p < 0.05) significantly reduced ALP levels, compared to DICLO. GAG release in the serum was also significantly lowered in OA-F2 treated group compared to DICLO. Radiological and histopathological observations showed cartilage protection by OA-F2. OA-F2 has upregulated SOD and GPx. Upregulated CAT expression was observed in OA-F2M and H. Considerable down-regulation of expression of MMP-3 and MMP-9 was observed in all the groups. Up-regulation of TIMP-1 was observed in rats treated with OA-F2L, H and DICLO.ConclusionOA-F2 has shown therapeutic effects in rat model of collagenase induced OA by demonstrating cartilage protection through controlling MMPs and improving anti-oxidant levels in arthritic synovium and is a potent candidate for further drug development and treatment for OA.

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“…Consequently, there is dramatically growing interest in herbal medicines among persons with RA and in the RA research community (Rao et al, 1999). In fact, herbal medicine is being widely used virtually around the world for treatment of rheumatic and arthritic diseases, and in recent decades considerable advances have been made in both clinical and basic research on the treatment of RA (Rao et al, 1999;Shin et al, 2003;Soeken et al, 2003;Chang et al, 2005;Park et al, 2005). Thus, herbal medicines constitute a potentially important avenue leading to novel therapeutic agents for RA that may not only prevent structural damage of arthritic joints caused by tissue and bone breakdown, but also be safe, relatively inexpensive, highly tolerated, and convenient for many patients.…”

Section: Introductionmentioning

confidence: 99%

Suppression of the onset and progression of collagen-induced arthritis in rats by QFGJS, a preparation from an anti-arthritic Chinese herbal formula

Cai

Zhou

Wong

et al. 2007

Journal of Ethnopharmacology

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Therapeutic effects of PG201, an ethanol extract from herbs, through cartilage protection on collagenase-induced arthritis in rabbits

Cited by 18 publications

References 37 publications

Suppressive effects of PG201, an antiarthritic botanical formulation, on lipopolysaccharide-induced inflammatory mediators in Raw264.7 cells

Suppressive effects of PG201, an antiarthritic botanical formulation, on lipopolysaccharide-induced inflammatory mediators in Raw264.7 cells

New herbal composition (OA-F2) protects cartilage degeneration in a rat model of collagenase induced osteoarthritis

Suppression of the onset and progression of collagen-induced arthritis in rats by QFGJS, a preparation from an anti-arthritic Chinese herbal formula

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