Therapeutic Effects of Thymoquinone in a Model of Neuropathic Pain

Abstract

BackgroundThe goal of our study was to determine the therapeutic effects of thymoquinone in a dose-dependent manner in a model of neuropathic pain following an experimentally applied spinal cord injury (SCI).MethodsFifty female adult Wistar albino rats weighing between 220 and 260 g were included in the study and were divided into 5 groups as follows: Group S (sham), Group C (control), Group T100 (100 mg/kg thymoquinone), Group T200 (200 mg/kg thymoquinone), and Group T400 (400 mg/kg thymoquinone). To begin the experiment, SCI was applied to all groups (with the exception of the sham group) following a mechanical and heat–cold test. Two weeks later, the mechanical and heat–cold tests were repeated, and a single normal saline dose was given to the sham and control groups, whereas 3 varying doses of thymoquinone were given to the other groups. The mechanical and heat–cold tests were repeated at 30, 60, 120, and 180 minutes after receiving thymoquinone. Finally, the animals were put to death via the removal of intracardiac blood. The levels of nitric oxide, total oxidant status, total antioxidant status, paraoxonase, malondialdehyde, tumor necrosis factor-α, and interleukin-1β were determined in all of the blood samples.ResultsThe withdrawal threshold and withdrawal latency values recorded from the mechanical and heat–cold allodynia measurements for all 3 thymoquinone groups were higher than that of the control group at all time points (ie, 30, 60, 120, and 180 minutes). There were no differences in these results between the 3 thymoquinone groups. The paraoxonase and total antioxidant status serum levels of all 3 thymoquinone groups were higher than those of the control group, whereas total oxidant status, nitric oxide, malondialdehyde, interleuken-1β, and tumor necrosis factor-α levels were lower in the 3 thymoquinone groups than in the control group.ConclusionsThymoquinone is beneficial for decreasing experimental neuropathic pain following SCI. However, increasing the dose does not change the effect.

Effect of ursolic acid in attenuating chronic constriction injury‐induced neuropathic pain in rats

“…Measurement of TOS may indicate the concentration of all free oxidant radicals caused by neuropathic pain‐related oxidative damage. TAS may be an important factor in providing protection from neurological damage caused by neuropathic pain‐related oxidative stress as it is difficult to measure each antioxidant separately . In the present study, TAS is elevated by UA administration in CCI rats in both spinal cord and sciatic nerve.…”

Cambios cinemáticos de la marcha en pacientes con artrosis de rodilla con diferentes descargas de peso

“…Las modificaciones con respecto a los rangos de movimiento en pacientes con artrosis de rodilla son diferentes, sobre todo en flexión de rodilla en cada fase de la marcha, esto como mecanismo de protección contra el dolor, ya que, si la flexión aumenta, el movimiento interarticular probablemente también lo hace incrementando el roce articular y así los síntomas (9). Sin embargo, los cambios en la excursión de movimiento dependen del estadio de la enfermedad, índice de masa corporal, edad y género, según Ko los rangos de movimiento no son estadísticamente diferentes en el plano sagital en las articulaciones de rodilla, tobillo y cadera entre personas sanas y con artrosis de rodilla en sus estadios 1 y 2, esto comparando la marcha usual, la marcha rápida y después de 30 minutos (10).…”

Erratum: Effect of Nigella sativa (Kalozira) oil on pain in Long Evans rats. J Bangladesh Soc Physiol 2018, June;13(1):1-7

“…x 34 x 34 cm 3 ) of the Plexiglass formalin test box in pairs for fifteen (15) minutes daily for four (4) days, and singly for three (3) days prior to the test 9,10 . On the day of experiment, the rat was intraperitoneally injected with 1% solution of Tween 20 or Nigella sativa oil 0.5 ml/kg or 1 ml/ kg or 1.5 ml/kg body weight, in accordance with the experimental paradigm being followed.…”

“…Previous studies demonstrated antinociceptive and anti-inflammatory effect of Nigella sativa, in animal models [12][13] . Recently, both single and long term supplementation of Nigella sativa has been demonstrated its good protective effects against nociceptive pain [14][15] .…”