Therapeutic Effects of Yijungtang on Atopic Dermatitis-like Skin Lesions of NC/Nga Mouse Induced by Mite Antigen

Abstract

ObjectivesThe purpose of this study is to investigate the effects of Yijungtang(YJT) on atopic dermatitis in an in-vitro and in-vivo experiment using a RBL-2H3 mast cells and a NC/Nga atopic dermatitis mouse. MethodsIn-vitro experiment, IL-4, IL-13 mRNA expression were evaluated by a real-time PCR, IL-4, IL-13 production by ELISA and transcription factor as GATA-1, GATA-2, NF-AT1, NF-AT2, AP-1 and NF-κB by western blotting. In-vivo experiment, clinical skin score we evaluated by, hematology and Serum total IgE and IgG1 of NC/Nga atopic dermatitis mouse, cytokine level, total number of cell, Immunohistochemical staining and Histological features of auxiliary lymph node(ALN), draining lymph node(DLN), peripheral blood mononuclear cells(PBMCs) and dorsal skin tissue in NC/Nga mouse. ResultsYJT decreased IL-4, IL-13 mRNA expression, IL-4, IL-13 production and prominently decreased the expression of mast cell specific transcription factors including GATA-2, NF-AT2, c-Fos and NF-κB. YJT oral administration reduced the levels of skin severity scores. It also decreased the level of inflammatory cytokines such as IL-5, IL-13, histamine and IgE in the serum. It elevated IFN-gamma level in the spleenocyte culture supernatant but decreased. CD3e ConclusionsThe anti-allergic activities of YJT may be mediated by down-regulation of Th2 cytokines, such as IL-4 and IL-13, through the regulation GATA-2, NF-AT2 and NF-κB transcription factors in mast cells. YJT would be regulate molecular mediators and immune cells which are functionally associated with atopic dermatitis induced in NC/Nga mice, and may play an important role in recovering AD symptoms.

Simultaneous quantification and inhibitory effect on LDL oxidation of the traditional Korean medicine, Leejung-tang

“…LJT has been used to treat various symptoms such as vomiting, stomach pain, chronic gastritis, and ulceration for a long time in Eastern countries [ 1 ]. Pharmacological studies of LJT have shown antiallergic [ 2 , 3 ], antitumor, immunomodulatory [ 4 ], acute toxicity [ 5 ], and gastroprotective [ 6 ] effects. Recently, the single herbs of LJT, including Ginseng Radix Alba [ 7 ], Zingiberis Rhizoma [ 8 ], and Glycyrrhizae Radix et Rhizoma [ 9 ] were reported to have an inhibitory effects against atherosclerosis.…”

Effects of Yijung-tang (YJT) on Experimental Hypothyroidism in Mice

“…이에 대 한 연구로는 氣血不足이라 보고 補氣補血 하는 十全大補湯 24) , 人參養榮湯 25) , 益氣補 血湯 26) 이 갑상선기능저하증에 미치는 영 향에 대한 연구, 腎陽虛로 변증하여 溫補 腎陽의 치법을 활용한 眞武湯 27) , 右歸飮 28) 에 대한 연구, 補陰하는 처방인 滋陰降火 . 현재까지 理中湯에 대한 연구로 는 항산화효과 13) , 알콜성 위염 보호 효과 14) , 항알러지 효과 15,16) , 진통 및 지사 효과 17) , 염증반응 억제 효과 (Fig. 3, 4).…”

“…理中湯은 ≪傷寒論≫ 10) 에 처음 수록된 처방으로 脾胃陽虛하고 陰이 盛한 증상 의 치료에 溫中散寒시키고, 氣虛, 脾虛로 인한 증후를 補氣健脾하는데 사용하는 처방으로서 11,12) 항산화효과 13) , 알콜성 위 염 보호 효과 14) , 항알러지 효과 15,16) , 진통 및 지사 효과 17) , 염증반응 억제 효과 18 (Fig. 3).…”

A Study on the Acute Toxicity of Leejung-tang(Lizhong-tang) and Fermented Leejung-tang(Lizhong-tang) Extract in ICR Mice

“…가 보고되었고, 이중탕을 과체중에 이용한 사례 7) , 항 알레르기 8) 및 아토피 피부염에 효과 9,10) 가 있는 것으로 알려지는 등 다방면으로 약리작용이 보고되고 있다. 이중탕은 본방(本方)뿐 아니라 다양한 질환에 가감응용(加減應用)될 수 있는 데 항암작용 11) , 대식세포의 산화분출(oxidative burst) 12,13) , 면역력 증가 14) , 비염치료 15) 에 효과가 있다고 보고되어 있다.…”