Therapeutic efficacy of magnesium after acoustic trauma caused by gunshot noise in guinea pigs

Sendowski, Isabelle; Raffin, F.; Braillon-Cros, Anne

doi:10.1080/00016480500312547

Cited by 23 publications

(15 citation statements)

References 20 publications

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“…To limit the individual variability, the methodology consisted of comparing the two ears of the same animal. Indeed, in previous experiments, we did not observe any significant differences in hair cell losses between the left and the right ear in the same animal exposed to impulse noise [7,8]. This methodology has been previously used in different studies [9,10].…”

Section: Discussionmentioning

confidence: 84%

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Intracochlear perfusion of leupeptin and z-VAD-FMK: influence of antiapoptotic agents on gunshot-induced hearing loss

Abaamrane¹,

Raffin²,

Schmerber

et al. 2011

Eur Arch Otorhinolaryngol

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The therapeutic efficiency of cochlear infusion of two anti-apoptotic substances: a potent calpain inhibitor, leupeptin and a caspase inhibitor, z-VAD-FMK was evaluated in guinea pigs after a gunshot noise-induced trauma (170 dB SPL). A preliminary study showed that hair cell apoptosis appeared within 7 days of the noise trauma. For each animal, one of the cochleae was perfused directly starting 1 h after the trauma with leupeptin or z-VAD-FMK for 7 days via a mini-osmotic pump whereas the other cochlea was untreated. ABR threshold shifts were measured over a 14-day recovery period. The functional hearing study was supplemented by histological analysis. Two days after the trauma significant differences were observed between threshold shifts in the z-VAD-FMK-treated and the non-treated ears. Cochleograms showed that hair cell losses were significantly lower in z-VAD-FMK-treated ears. Regarding the leupeptin treatment, no significant difference between treated and non-treated ears was observed. This work indicates that early direct infusion of z-VAD-FMK into the cochlea accelerates hearing recovery and reduces hair cell loss after gunshot noise-induced trauma. These results suggest that the gunshot noise-induced trauma may involve the caspase pathway rather than the calpain pathway in the apoptotic process.

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Section: Discussionmentioning

confidence: 84%

“…Two days after gunshot noise exposure, z-VAD-FMK-treated ears displayed significantly lower hearing loss at high frequencies (8,16,19 and 22 kHz). On days 7 and 14, hearing loss was equivalent in treated and non-treated ears.…”

Section: Discussionmentioning

confidence: 98%

Intracochlear perfusion of leupeptin and z-VAD-FMK: influence of antiapoptotic agents on gunshot-induced hearing loss

Abaamrane¹,

Raffin²,

Schmerber

et al. 2011

Eur Arch Otorhinolaryngol

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“…Threshold shift reduction 7 days after acoustic trauma in guinea pigs was confirmed using DPOAE (Haupt et al, 2003). In another study (Sendowski et al, 2006c) it has been shown that a 7-day post-trauma magnesium treatment reduced auditory threshold shift measured 7 days after gunshot noise exposure. However, this improvement was temporary, suggesting that it could be potentially beneficial to prolong magnesium administration.…”

Section: Introductionmentioning

confidence: 90%

Long-term administration of magnesium after acoustic trauma caused by gunshot noise in guinea pigs

et al. 2009

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“…Der mechanische Schaden entsteht vermutlich durch eine über-schießende Auslenkung der Basilarmembran. Es kommt zum Ablösen von Stereozilien von der Tektorialmembran, einer Diskonnektion der interziliären Verbindungen bis hin zu Zerreißungen der Basilarmembran [3,33]. Hamernik et al [14] zeigten, dass die Zilien der inneren Haarzellen im Vergleich zu denen der äuße-ren Haarzellen deutlich weniger Schä-den durch akustische Traumata aufwiesen.…”

Section: Pathophysiologieunclassified

“…Die hieraus resultierende Ischämie und Sauerstoffunterversorgung des Corti-Organs führt ebenfalls zu einer Störung der Ionenhomöo-mostase in der Endolymphe [3,21]. Eine darauf folgende exzessive Glutamatfreisetzung und Radikalenstehung kommt schädigend hinzu [33].…”

Section: Pathophysiologieunclassified

Update: blast and explosion trauma

Weyer

Praetorius

Tisch

2011

HNO

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In recent decades, acoustic shock and explosion traumas have increased in frequency in the general population. Beside the use of fireworks and firearms, airbag ignitions and explosions caused by terror or suicidal acts are also relevant. Depending on duration and strength of the sound pressure affecting the human ear, isolated inner ear damage or additional ear drum perforation and interruption of the middle ear ossicle chain can result. By means of otoscopy, pure tone audiometry, measurement of otoacoustic emissions, and other neurootological examinations, the severity of the trauma can be determined. With prompt and adequate therapy, permanent hearing loss can be minimized. In particular, the measurement of otoacoustic emissions allows conclusions to be made on the functionality of the outer hair cells which are damaged first in most cases. Histological investigations on noise-exposed cochleas show extensive damage to the outer hair cells in the frequency range between 1.0 and 4.0 kHz, which correlates well with audiometric measurements.

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Therapeutic efficacy of magnesium after acoustic trauma caused by gunshot noise in guinea pigs

Cited by 23 publications

References 20 publications

Intracochlear perfusion of leupeptin and z-VAD-FMK: influence of antiapoptotic agents on gunshot-induced hearing loss

Intracochlear perfusion of leupeptin and z-VAD-FMK: influence of antiapoptotic agents on gunshot-induced hearing loss

Long-term administration of magnesium after acoustic trauma caused by gunshot noise in guinea pigs

Update: blast and explosion trauma

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