Therapeutic enhancement of radiation and immunomodulation by gold nanoparticles in triple negative breast cancer

Janic, Branislava; Brown, Stephen L.; Neff, R.A.; Liu, Fangchao; Mao, Guangzhao; Chen, Yalei; Jackson, LaToya; Chetty, Indrin J.; Movsas, Benjamin; Wen, Ning

doi:10.1080/15384047.2020.1861923

Cited by 38 publications

(23 citation statements)

References 83 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 25 , 26 Radiotherapy is also a treatment that can induce ICD in tumour cells, and the presence of radiotherapy sensitizer AuNPs may enhance this effect. 46 However, the combination of the two can induce stronger ICD effects in tumour cells and induce the activation and maturation of BMDCs. Tumour vaccine experiment results have shown that the injection of dying necroptotic cancer cells directly leads to more effective control of tumour growth in mice.…”

Section: Discussionmentioning

confidence: 99%

Immunogenic Cell Death Induced by Chemoradiotherapy of Novel pH-Sensitive Cargo-Loaded Polymersomes in Glioblastoma

He¹,

Ding²,

Chen³

et al. 2021

IJN

View full text Add to dashboard Cite

Background Inducing the immunogenic cell death of tumour cells can mediate the occurrence of antitumour immune responses and make the therapeutic effect more significant. Therefore, the development of treatments that can induce ICD to destroy tumour cells most effectively is promising. Previously, a new type of pH-sensitive polymersome was designed for the treatment of glioblastoma which represents a promising nanoplatform for future translational research in glioblastoma therapy. In this study, the aim of this work was to analyse whether chemoradiotherapy of the novel pH-sensitive cargo-loaded polymersomes can induce ICD. Methods Cell death in U87-MG and G422 cells was induced by Au-DOX@PO-ANG, and cell death was analysed by CCK-8 and flow cytometry. The release of CRT was determined by using laser scanning confocal microscopy and flow cytometry. ELISA kits were used to detect the release of HMGB1 and ATP. The dying cancer cells treated with different treatments were cocultured with bone-marrow-derived dendritic cells (BMDCs), and then flow cytometry was used to determine the maturation rate of BMDCs (CD11c+CD86+CD80+) to analyse the in vitro immunogenicity. Tumour vaccination experiments were used to evaluate the ability of Au-DOX@PO-ANG to induce ICD in vivo. Results We determined the optimal treatment strategy to evaluate the ability of chemotherapy combined with radiotherapy to induce ICD and dying cancer cells induced by Au-DOX@PO-ANG+RT could induce calreticulin eversion to the cell membrane, promote the release of HMGB1 and ATP, and induce the maturation of BMDCs. Using dying cancer cells induced by Au-DOX@PO-ANG+RT, we demonstrate the efficient vaccination potential of ICD in vivo. Conclusion These results identify Au-DOX@PO-ANG as a novel immunogenic cell death inducer in vitro and in vivo that could be effectively combined with RT in cancer therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Immunogenic Cell Death Induced by Chemoradiotherapy of Novel pH-Sensitive Cargo-Loaded Polymersomes in Glioblastoma

He¹,

Ding²,

Chen³

et al. 2021

IJN

View full text Add to dashboard Cite

show abstract

“…RT exerts cytotoxic and cytostatic effects via DNA damage by fractionated focal irradiation ( 81 ). However, these irradiations are unable to differentiate tumor cells from normal cells which lead to unnecessary destruction of surrounding healthy tissues ( 82 ). Additionally, its tumoricidal ability is often limited by the maximum deposited dosage and that has always remained a challenge among radiologists ( 83 ).…”

Section: Radiation Therapy With Gold Nanoparticlesmentioning

confidence: 99%

“…Generation of reactive oxygen species (ROS) can be induced by the Auger electron production from the surface of AuNPs ( 20 ). By coupling with an active targeting strategy or by the passive targeting of the EPR effect alone, using AuNPs as radiosensitizers allows the reduction of total RT dosage whilst increasing the local RT dosage to tumor sites ( 82 ). For example, conjugation of Tz to PEG-AuNPs (Tz-PEG-AuNPs) allowed intracellular uptake of AuNPs into SK-BR-3 human breast cancer cells, which then proceeded to cause DNA double-strand breaks by 3.3 to 5.1 times more than RT alone or RT with PEG-AuNPs ( 88 ).…”

Section: Radiation Therapy With Gold Nanoparticlesmentioning

confidence: 99%

“…This process is especially useful against immunologically cold tumors, like triple negative breast cancer, because the increase in TME immunogenicity was found to be correlated with better prognosis due to improved patient responses to immunotherapy and chemotherapy ( 102 ). RT with 14 nm AuNPs showed enhanced RT efficacy, and induced ICD that resulted in significant macrophage infiltration, and hence improved OS in an MDA-MB-231 human breast cancer murine model ( 82 ).…”

Section: Radiation Therapy With Gold Nanoparticlesmentioning

confidence: 99%

See 1 more Smart Citation

Harnessing the Immunogenic Potential of Gold Nanoparticle-Based Platforms as a Therapeutic Strategy in Breast Cancer Immunotherapy: A Mini Review

et al. 2022

View full text Add to dashboard Cite

Breast cancer remains the most common malignancy among women worldwide. Although the implementation of mammography has dramatically increased the early detection rate, conventional treatments like chemotherapy, radiation therapy, and surgery, have significantly improved the prognosis for breast cancer patients. However, about a third of treated breast cancer patients are known to suffer from disease recurrences and progression to metastasis. Immunotherapy has recently gained traction due to its ability to establish long-term immune surveillance, and response for the prevention of disease recurrence and extension of patient survival. Current research findings have revealed that gold nanoparticles can enhance the safety and efficacy of cancer immunotherapy, through their unique intrinsic properties of good biocompatibility, durability, convenient surface modification, as well as enhanced permeability and retention effect. Gold nanoparticles are also able to induce innate immune responses through the process of immunogenic cell death, which can lead to the establishment of lasting adaptive immunity. As such gold nanoparticles are considered as good candidates for next generation immunotherapeutic strategies. This mini review gives an overview of gold nanoparticles and their potential applications in breast cancer immunotherapeutic strategies.

show abstract

“…Auaided radiotherapy (RT) is useful for making tumors more sensitive to radiation damage because of an enhancement of the radiation dose in the tumor region. This enhancement occurs primarily due to the photoelectric cross section which is much higher for Au (Z = 79) as compared to tumor tissue (Bromma et al 2020;Cho 2005;Choi et al 2020;Janic et al 2021;Chow 2020a, 2020b). Hainfeld and coworkers (Hainfeld et al 2010) demonstrated that Au NPs are non-toxic up to a mass concentration of 20 mg/g when studying a murine squamous cell carcinoma cell line (SCC VII).…”

Section: Introductionmentioning

confidence: 99%

Gold-nanoparticle-enriched breast tissue in breast cancer treatment using the INTRABEAM® system: a Monte Carlo study

Tegaw

Geraily

Gholami

et al. 2021

Radiat Environ Biophys

View full text Add to dashboard Cite

Using a 50-kV INTRABEAM ® system after breast-conserving surgery, breast skin injury and long treatment time remain the challenging problems when large-size spherical applicators are used. This study has aimed to address these problems using gold (Au) nanoparticles (NPs). For this, surface and isotropic doses were measured using a Gafchromic EBT3 film and a water phantom. The particle propagation code EGSnrc/Epp was used to score the corresponding doses using a geometry similar to that used in the measurements. The simulation was validated using a gamma index of 2%/2 mm acceptance criterion in the gamma analysis. After validation Au-NP-enriched breast tissue was simulated to quantify any breast skin dose reduction and shortening of treatment time. It turned out that the gamma value deduced for validation of the simulation was in an acceptable range (i.e., less than one). For 20 mg-Au/g-breast tissue, the calculated Dose Enhancement Ratio (DER) of the breast skin was 0.412 and 0.414 using applicators with diameters of 1.5 cm and 5 cm, respectively. The corresponding treatment times were shortened by 72.22% and 72.30% at 20 mg-Au/g-breast tissue concentration, respectively. It is concluded that Au-NP-enriched breast tissue shows significant advantages, such as reducing the radiation dose received by the breast skin as well as shortening the treatment time. Additionally, the DERs were not significantly dependent on the size of the applicators.

show abstract

Therapeutic enhancement of radiation and immunomodulation by gold nanoparticles in triple negative breast cancer

Cited by 38 publications

References 83 publications

Immunogenic Cell Death Induced by Chemoradiotherapy of Novel pH-Sensitive Cargo-Loaded Polymersomes in Glioblastoma

Immunogenic Cell Death Induced by Chemoradiotherapy of Novel pH-Sensitive Cargo-Loaded Polymersomes in Glioblastoma

Harnessing the Immunogenic Potential of Gold Nanoparticle-Based Platforms as a Therapeutic Strategy in Breast Cancer Immunotherapy: A Mini Review

Gold-nanoparticle-enriched breast tissue in breast cancer treatment using the INTRABEAM® system: a Monte Carlo study

Contact Info

Product

Resources

About