The purpose of this study is to establish that certain uterine disorders may play a significant role in female buffalo infertility. A total of 340 reproductive tracts of female buffaloes have a previous history of infertility was collected from different farms in Egypt through the period of two years. Uteri have been visually and manually inspected as well as examined bacteriologically and histologically. Several uterine lesions either external or internal were noted in 78/340 animals (22.94%) as follows: internal lesions as endometritis in 33 animals (9.71%), metritis in 11 animals (3.24%), endometrial hyperplasia in 7 animals (2.06%), adenomyosis in 5 animals (1.47%) and fibroma in 3 animals (0.88%). External uteri lesions as perimetrial nodules in 10 animals (2.94 %). Perimetrial cysts in 9 animals (2.65%). Furthermore, many bacteria were isolated and the most common was Klebsiella oxytoca, Proteus vulgaris, Proteus vulgaris and Enterobacter aerogenes. The conclusion is that uterine disorders play a crucial role in female buffaloes' infertility. It is highly recommended to use specific clinical parameters and biopsy techniques for early diagnosis in order to proceed with the appropriate treatment.