Therapeutic mechanism of human neural stem cell-derived extracellular vesicles against hypoxia-reperfusion injury in vitro

Liu, Qingyue; Tan, Yi Jayne; Qu, Tingyu; Zhang, Jianhui; Duan, Xuexia; Xu, Hongpeng; Mu, Yao; Ma, Huimin; Wang, Fengshan

doi:10.1016/j.lfs.2020.117772

Cited by 29 publications

(35 citation statements)

References 36 publications

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“…EVs are cell-derived small particles with a stable bilayer membrane structure ( Loch-Neckel et al, 2022 ). The diameter of EVs were approximately 50–150 nm with a “cup” shape morphology ( Liu et al, 2020 ). Currently, several techniques are being used for the detection and characterization of EVs, including nanoparticle tracking analysis, electron microscopy, immunogold-electron microscopy, atomic force microscopy, localized surface plasmon resonance biosensor, etc ( Thakur et al, 2017 ; Thakur et al, 2017 ; Qiu et al, 2018 ; Thakur et al, 2020c ; Thakur et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Circulating galectin-3 promotes tumor-endothelium-adhesion by upregulating ICAM-1 in endothelium-derived extracellular vesicles

Wang

Zheng

et al. 2022

Front. Pharmacol.

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The adhesion of tumor cells to vascular endothelial cells is an important process of tumor metastasis. Studies have shown that tumor could educate vascular endothelial cells to promote tumor metastasis through many ways. However, the effect of tumor cells on the functions of vascular endothelial cells-derived extracellular vesicles (H-EVs) and the mechanisms underlying their effects in tumor-endothelium adhesion in metastasis remain mysterious. In this study, we found that H-EVs promoted the adhesion of triple negative breast cancer cell to endothelial cells and cirGal-3 enhanced the adhesion-promoting effects of H-EVs. The underlying mechanism was related to the upregulation of glycolysis in endothelial cells induced by cirGal-3 which led to the increase of the ICAM-1 expression and its transmission to MDA-MB-231 cells by H-EVs. Targeting of cirGal-3 or glycolysis of vascular endothelium in breast cancer therefore represents a promising therapeutic strategy to reduce metastasis.

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Section: Discussionmentioning

confidence: 99%

Circulating galectin-3 promotes tumor-endothelium-adhesion by upregulating ICAM-1 in endothelium-derived extracellular vesicles

Wang

Zheng

et al. 2022

Front. Pharmacol.

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“…Thus, a possible therapy is to administer exogenous NSCs obtained from neuroectoderm in fetuses or from the neurogenic sites in adults, after they have been cultured and exposed to basic fibroblast growth factor and epidermal growth factor [ 110 , 111 ]. Unfortunately, the host brain often rejects transplant NSCs [ 112 ], although it has been observed that delivery of human NSC-derived extracellular vesicles improves transplantation outcomes by stimulating nuclear localization of Nrf2, which subsequently decreases oxidative stress and stimulates axon elongation [ 113 , 114 ]. The following section investigates drug therapy that may be used concomitantly with NSC therapy to improve outcomes in patients suffering from cerebrovascular disease.…”

Section: Current Nrf2-directed Treatment Modalitiesmentioning

confidence: 99%

The Role of Concomitant Nrf2 Targeting and Stem Cell Therapy in Cerebrovascular Disease

et al. 2022

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Despite the reality that a death from cerebrovascular accident occurs every 3.5 min in the United States, there are few therapeutic options which are typically limited to a narrow window of opportunity in time for damage mitigation and recovery. Novel therapies have targeted pathological processes secondary to the initial insult, such as oxidative damage and peripheral inflammation. One of the greatest challenges to therapy is the frequently permanent damage within the CNS, attributed to a lack of sufficient neurogenesis. Thus, recent use of cell-based therapies for stroke have shown promising results. Unfortunately, stroke-induced inflammatory and oxidative damage limit the therapeutic potential of these stem cells. Nuclear factor erythroid 2-related factor 2 (Nrf2) has been implicated in endogenous antioxidant and anti-inflammatory activity, thus presenting an attractive target for novel therapeutics to enhance stem cell therapy and promote neurogenesis. This review assesses the current literature on the concomitant use of stem cell therapy and Nrf2 targeting via pharmaceutical and natural agents, highlighting the need to elucidate both upstream and downstream pathways in optimizing Nrf2 treatments in the setting of cerebrovascular disease.

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“…NSC-derived exosomes have the effect of promoting nerve regeneration. An in vitro study has shown that exosomes released from NSCs can promote neuronal axonal elongation and neural network reconstruction under both normal and hypoxia-reperfusion injury conditions (Liu Q. et al, 2020). Morton et al (2018) demonstrated that exosomes released by NSCs of neonatal SVZ can be selectively ingested by microglia.…”

Section: Exosomes In Nsc-neuron/glial Cell Communicationmentioning

confidence: 99%

The Emerging Role of Neural Cell-Derived Exosomes in Intercellular Communication in Health and Neurodegenerative Diseases

Huo

et al. 2021

Front. Neurosci.

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Intercellular communication in the central nervous system (CNS) is essential for brain growth, development, and homeostasis maintenance and, when dysfunctional, is involved in the occurrence and development of neurodegenerative diseases. Increasing evidence indicates that extracellular vesicles, especially exosomes, are critical mediators of intercellular signal transduction. Under physiological and pathological conditions, neural cells secret exosomes with the influence of many factors. These exosomes can carry specific proteins, lipids, nucleic acids, and other bioactive substances to the recipient cells to regulate their function. Depending on the CNS environment, as well as the origin and physiological or pathological status of parental cells, exosomes can mediate a variety of different effects, including synaptic plasticity, nutritional metabolic support, nerve regeneration, inflammatory response, anti-stress effect, cellular waste disposal, and the propagation of toxic components, playing an important role in health and neurodegenerative diseases. This review will discuss the possible roles of exosomes in CNS intercellular communication in both physiologic and neurodegenerative conditions.

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Therapeutic mechanism of human neural stem cell-derived extracellular vesicles against hypoxia-reperfusion injury in vitro

Cited by 29 publications

References 36 publications

Circulating galectin-3 promotes tumor-endothelium-adhesion by upregulating ICAM-1 in endothelium-derived extracellular vesicles

Circulating galectin-3 promotes tumor-endothelium-adhesion by upregulating ICAM-1 in endothelium-derived extracellular vesicles

The Role of Concomitant Nrf2 Targeting and Stem Cell Therapy in Cerebrovascular Disease

The Emerging Role of Neural Cell-Derived Exosomes in Intercellular Communication in Health and Neurodegenerative Diseases

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