Therapeutic perspectives of high pulse repetition rate electroporation

de Caro, Alexia; Talmont, Franck; Rols, Marie-Pierre; Golzio, Muriel; Kolosnjaj-Tabi, Jelena

doi:10.1016/j.bioelechem.2023.108629

Bioelectrochemistry

2024

DOI: 10.1016/j.bioelechem.2023.108629

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Therapeutic perspectives of high pulse repetition rate electroporation

Alexia de Caro,

Franck Talmont,

Marie-Pierre Rols

et al.

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2024

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Cited by 2 publications

References 127 publications

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Cardiac selectivity in pulsed field ablation

Meng,

Jin,

Liu

2024

Current Opinion in Cardiology

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Purpose of review This review examines the selective cardiac injury induced by pulsed electric fields during atrial fibrillation ablation. It consolidates findings from both preclinical and clinical studies on cardiac selectivity and explores the potential mechanisms behind this selectivity. Recent findings Preclinical studies indicate that pulsed electric fields cause significantly more myocardial injury compared with other tissues. Clinical studies have similarly shown that complication rates for pulsed field ablation are notably lower than those for radiofrequency and cryoballoon ablation. Summary Pulsed field ablation demonstrates a notable selectivity for myocardial injury, likely because of the unique functional and metabolic characteristics of cardiomyocytes. This review delves into the underlying principles of cardiac selectivity and proposes future directions for improving this selectivity. It is important to note that while pulsed field ablation shows promise, its cardiac selectivity is not absolute, as some complications still occur, necessitating further research.

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Cardiac selectivity in pulsed field ablation

Meng,

Jin,

Liu

2024

Current Opinion in Cardiology

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Threshold Interphase Delay for Bipolar Pulses to Prevent Cancellation Phenomenon during Electrochemotherapy

Malyško-Ptašinskė,

Nemeikaitė-Čėnienė,

Radzevičiūtė-Valčiukė

et al. 2024

IJMS

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Electroporation-based procedures employing nanosecond bipolar pulses are commonly linked to an undesirable phenomenon known as the cancelation effect. The cancellation effect arises when the second pulse partially or completely neutralizes the effects of the first pulse, simultaneously diminishing cells’ plasma membrane permeabilization and the overall efficiency of the procedure. Introducing a temporal gap between the positive and negative phases of the bipolar pulses during electroporation procedures may help to overcome the cancellation phenomenon; however, the exact thresholds are not yet known. Therefore, in this work, we have tested the influence of different interphase delay values (from 0 ms to 95 ms) using symmetric bipolar nanoseconds (300 and 500 ns) on cell permeabilization using 10 Hz, 100 Hz, and 1 kHz protocols. As a model mouse hepatoma, the MH-22a cell line was employed. Additionally, we conducted in vitro electrochemotherapy with cisplatin, employing reduced interphase delay values (0 ms and 0.1 ms) at 10 Hz. Cell plasma membrane permeabilization and viability dependence on a variety of bipolar pulsed electric field protocols were characterized. It was shown that it is possible to minimize bipolar cancellation, enabling treatment efficiency comparable to monophasic pulses with identical parameters. At the same time, it was highlighted that bipolar cancellation has a significant influence on permeabilization, while the effects on the outcome of electrochemotherapy are minimal.

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Therapeutic perspectives of high pulse repetition rate electroporation

Cited by 2 publications

References 127 publications

Cardiac selectivity in pulsed field ablation

Cardiac selectivity in pulsed field ablation

Threshold Interphase Delay for Bipolar Pulses to Prevent Cancellation Phenomenon during Electrochemotherapy

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