Therapeutic potential of a distinct population of human amniotic fluid mesenchymal stem cells and their secreted molecules in mice with acute hepatic failure

Zagoura, Dimitra; Roubelakis, Maria G.; Bitsika, Vasiliki; Trohatou, Ourania; Pappa, Kalliopi I.; Kapelouzou, Alkistis; Antsaklis, A.; Anagnou, Nicholas P.

doi:10.1136/gutjnl-2011-300908

Cited by 159 publications

(175 citation statements)

References 42 publications

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“…Additionally, it has been indicated that liver recovery might be induced through anti-inflammatory mediators such as interleukins IL-10, IL-1ra, IL-13, and IL-27 in hepatic progenitor CM. Blocking studies of IL-10 secretion from these cells confirmed the therapeutic potential of this cytokine in ALF mouse models [32].…”

Section: Discussionmentioning

confidence: 80%

Effect of Secreted Molecules of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells on Acute Hepatic Failure Model

Lotfinia

Kadivar

Piryaei

et al. 2016

Stem Cells and Development

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Adult tissue-derived mesenchymal stem cells (MSCs) show tremendous promise for a wide array of therapeutic applications predominantly through paracrine activity. Recent reports showed that human embryonic stem cell (ESC)-derived MSCs are an alternative for regenerative cellular therapy due to manufacturing large quantities of MSCs from a single donor. However, no study has been reported to uncover the secretome of human ESCMSCs as treatment of an acute liver failure (ALF) mouse model. We demonstrated that human ESC-MSCs showed similar morphology and cell surface markers compared with bone marrow-derived MSCs. ESC-MSCs exhibited a higher growth rate during early in vitro expansion, along with adipogenic and osteogenic differentiation potential. Treatment with ESC-MSC-conditioned medium (CM) led to statistically significant enhancement of primary hepatocyte viability and increased immunomodulatory interleukin-10 secretion from lipopolysaccharide-induced human blood mononuclear cells. Analysis of the MSCs secretome by a protein array screen showed an association between higher frequencies of secretory proteins such as vascular endothelial growth factor (VEGF) and regulation of cell proliferation, cell migration, the development process, immune system process, and apoptosis. In this thioacetamide-induced mouse model of acute liver injury, we observed that systemic infusion of VEGF led to significant survival. These data have provided the first experimental evidence of the therapeutic potential of human ESC-MSC-derived molecules. These molecules show trophic support to hepatocytes, which potentially creates new avenues for the treatment of ALF, as an inflammatory condition.

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Section: Discussionmentioning

confidence: 80%

Effect of Secreted Molecules of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells on Acute Hepatic Failure Model

Lotfinia

Kadivar

Piryaei

et al. 2016

Stem Cells and Development

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show abstract

“…These findings suggest that partial in vitro induction of stem cells, which actives stem cells and preserves their paracrine capability, is the better treatment option [33].…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Strategies of Stem Cell Transplantation for Liver Cirrhosis

Fan¹

2017

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Liver transplantation is widely regarded as the most effective therapy for end-stage liver diseases. However, stem cell-based therapy is being developed as a promising strategy which offers a number of benefits as it is minimally invasive and associated with low immunogenicity and low cost. This paper will review the major clinical issues surrounding the use of stem cell therapy for managing cirrhosis, such as discussing the selection of appropriate subtypes of bone marrow stem cells and the need for pre-differentiation into hepatocyte-like cells prior to transplantation, and providing an overview of the methods to improve cell viability and to prevent the exacerbation of cirrhosis. The role of human umbilical cord blood stem cells and amniotic epithelial cells for the treatment of liver disease will be also introduced.

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“…Mesenchymal stem cells constitute another type of somatic stem cells, mainly characterized by their ability to differentiate into numerous tissues, including bone, cartilage and adipose tissue [7]. Specifically, it is this multipotent differentiation potential that is being used as a functional criterion for the definition of MSCs, since there are no specific markers assigned for the identification of this cell population.…”

Section: In Vivomentioning

confidence: 99%

“…In that sense, MSCs are not genetically engineered in order to improve the phenotype of the respective tissue they derive from, but are used to carry a therapeutic gene to a damaged tissue. Recently, Zagoura and colleagues [7] have documented the effects of genetically engineered MSCs for the treatment of acute liver failure in an animal model, utilizing human amniotic fluid MSCs. Another major advantage of MSCs comprises their innate tropism to solid tumors in vivo that readily qualifies these cells as the perfect carriers for anti-tumour agents [8].…”

Section: In Vivomentioning

confidence: 99%

Genetic Manipulation of Stem Cells

Papanikolaou¹,

Pappa²

2012

Gynecol Obstet (Sunnyvale)

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Stem cells have the remarkable potential for self-renewal and differentiation into many cell types in the body during early life and development. In addition, in many tissues they constitute a source of internal repair system, dividing essentially without limit to replenish damaged or dead cells. After division, each new cell has the potential either to retain the stem cell status or to differentiate to a more specialized cell type, such as a red blood cell, a brain cell or a heart cell.Until recently, three types of stem cells from animals and humans have been characterized, i.e. embryonic stem cells, fetal stem cells and somatic adult stem cells. However, in late 2007, researchers accomplished another breakthrough by identifying conditions that allow some specialized adult cells to be "reprogrammed" genetically to assume a stem cell-like state. These cells, called induced pluripotent stem cells (iPSCs), express genes and factors important for maintaining the unique properties and features of embryonic stem cells.This review analyzes the mechanisms of genetic manipulation of stem cells, including the transfer of therapeutic genes into patients' cells via recombinant viral vectors for gene therapy purposes and discusses the mechanisms of generation and the resulting properties of induced pluripotent stem cells.

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Therapeutic potential of a distinct population of human amniotic fluid mesenchymal stem cells and their secreted molecules in mice with acute hepatic failure

Abstract: Human spindle-shaped AF-MSCs or HPL cells might be valuable tools to induce liver repair and support liver function by cell transplantation. More importantly, the factors they release may also play an important role in cell treatment in diseases of the liver.

Cited by 159 publications

References 42 publications

Effect of Secreted Molecules of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells on Acute Hepatic Failure Model

Effect of Secreted Molecules of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells on Acute Hepatic Failure Model

Therapeutic Strategies of Stem Cell Transplantation for Liver Cirrhosis

Genetic Manipulation of Stem Cells

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