Therapeutic Potential of CPPs

Langel, Ülo

doi:10.1007/978-981-13-8747-0_12

Cited by 2 publications

(1 citation statement)

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“…Additionally, once the proteins are delivered, they may be vulnerable to degradation or loss of activity within the cell, ultimately impacting their therapeutic efficacy [14]. Endocytic delivery pathways, for example, may result in endosomal entrapment and potential degradation, highlighting the need for endoplasmic agents to facilitate efficient cargo release [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Enhancing Cellular Uptake of Native Proteins through Bio-Orthogonal Conjugation with Chemically Synthesized Cell-Penetrating Peptides

Nebogatova,

Porosk,

Härk

et al. 2024

Pharmaceutics

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The potential for native proteins to serve as a platform for biocompatible, targeted, and personalized therapeutics in the context of genetic and metabolic disorders is vast. Nevertheless, their clinical application encounters challenges, particularly in overcoming biological barriers and addressing the complexities involved in engineering transmembrane permeability. This study is dedicated to the development of a multifunctional nanoentity in which a model therapeutic protein is covalently linked to a cell-penetrating peptide, NickFect 55, with the objective of enhancing its intracellular delivery. Successful binding of the nanoentity fragments was achieved through the utilization of an intein-mediated protein-trans splicing reaction. Our research demonstrates that the fully assembled nanoentity-containing protein was effectively internalized by the cells, underscoring the potential of this approach in overcoming barriers associated with protein-based therapeutics for the treatment of genetic disorders.

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