Therapeutic potential of Smilax fluminensis ethanolic extract: antitumoral activity in murine melanoma cells

Fetter, Bruna Z; Dourado, Doroty Mesquita; Bogo, Danielle; Matias, Rosemary; Guterres, Zaira Rosa

doi:10.1007/s11010-021-04272-5

Cited by 2 publications

(3 citation statements)

References 29 publications

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“…In recent decades, advances in cancer molecular biology have identified several biological compounds capable of inhibiting cancer cell growth with improved efficacy and selectivity. 5 − 11 Structural diversity and associated synergistic effects, high efficiency, availability, and excellent biocompatibility are the advantages of plants as medicinal resources. Traditional plants contain phytochemical compounds, which are mainly secondary metabolites used by plants to ensure their survival and fertility.…”

Section: Introductionmentioning

confidence: 99%

“…Natural resources such as plants have long been considered as a source of chemotherapeutic or chemopreventive agents against cancer. − However, due to the increasing resistance of certain tumors and the severe side effects of conventional chemotherapy, new pharmacological molecules are needed. In recent decades, advances in cancer molecular biology have identified several biological compounds capable of inhibiting cancer cell growth with improved efficacy and selectivity. − Structural diversity and associated synergistic effects, high efficiency, availability, and excellent biocompatibility are the advantages of plants as medicinal resources. Traditional plants contain phytochemical compounds, which are mainly secondary metabolites used by plants to ensure their survival and fertility.…”

Section: Introductionmentioning

confidence: 99%

“…Nowadays, much attention has been paid to the antitumor effect of saponins, and several publications have appeared. − , Some possible reasons for the antitumor effect of saponins are the formation of pores in cell membranes and thus increased permeabilization, induction of apoptosis, inhibition of angiogenesis and metastasis, and reduction of drug efflux . Although many studies have shown that saponins damage tumors rather than attack normal organs, their use as antitumor agents in clinical trials is a major obstacle because of their high organ toxicity .…”

Section: Introductionmentioning

confidence: 99%

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Use of Saponinosomes from Ziziphus spina-christi as Anticancer Drug Carriers

et al. 2022

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Saponins are plant glycosides with different structures and biological activities, such as anticancer effects. Ziziphus spina-christi is a plant rich in saponin, and this compound is used to treat malignant melanoma in the present study. Nanophytosomes can be used as an advantageous nanodrug delivery system for plant extracts. The aim of this work is to use the saponin-rich fraction (SRF) from Z. spina-christi and prepare SRF-loaded nanophytosomes (saponinosomes) and observe the in vitro and in vivo effects of these carriers. First, the SRF was obtained from Z. spina-christi by a solvent–solvent fractionation method. Then, Fourier transform infrared (FTIR) analyses were performed to confirm the presence of saponins in the extracted material. Subsequently, the saponinosomes were prepared by the solvent injection method (ether injection method) using a 1:1:1 ratio of lecithin/cholesterol/SRF in the mixture. Characterization of the prepared saponinosomes was performed by FTIR, dynamic light scattering (DLS), field-emission scanning electron microscopy (FE-SEM), and atomic force microscopy (AFM) analyses. In addition, a UV–vis spectrophotometer was used to determine the entrapment efficiency (EE) and in vitro release of the SRF. Finally, cell cytotoxicity of the different formulations was evaluated using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay on both mouse melanoma cells (B16F10) and fibroblasts (L929). Using DLS, AFM, and FE-SEM analyses, the particle size was determined to be 58 ± 6 nm with a zeta potential of −32 ± 2 mV. The calculated EE was 85 ± 3%. The results of the in vitro release profile showed that 68.2% of the SRF was released from the saponinosome after 48 h. The results of the MTT assay showed that the SRF and saponinosomes have high toxicity on B16F10 melanoma cells, but saponinosomes showed a significant decrease in cytotoxicity on L929 fibroblast cells compared with that of the SRF. Our results indicate that the SRF from Z. spina-christi has anticancer activity, and the saponinosomes prepared in this work can control tumor growth, improve therapeutic efficacy, and reduce the side effects of saponins.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Use of Saponinosomes from Ziziphus spina-christi as Anticancer Drug Carriers

et al. 2022

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Characterisation of bioactive compounds from leaves of Smilax fluminensis Steud. by gc-MS and LC-DAD-MS, and analysis of its antioxidant and ecotoxicological activities

Azevedo,

Fonseca,

Castro

et al. 2024

Natural Product Research

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Smilax fluminensisSteud. is distributed in Central and South America, commonly named as "salsaparilha" or "japicanga". In the present work, the chemical composition was determined, antioxidant and cytotoxic effects were evaluated for the ethanol extract (EE) and fractions from leaves. Fatty acid esters and phytol were characterized in the hexane (HEX) fraction. O-glycosylated flavonols and flavones, caffeic acid derivatives, and steroidal saponins were annotated for EE, and dichloromethane (DCM), ethyl acetate (AC), and hydroethanol (HE) fractions. The samples contain flavonoids and phenolic compounds, and the AC fraction displayed the biggest concentration of these substances.Antioxidant potential was observed in all samples, being especially pronounced in the AC fraction by DPPH and FRAP assays, with IC50 values of 8.18 and 2.35 g/mL, respectively. AC and HEX fractions showed 35% and 5% lethality at 1000 μg/mL, in the Artemia salina assay, and the other samples did not show a toxic effect.

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Therapeutic potential of Smilax fluminensis ethanolic extract: antitumoral activity in murine melanoma cells

Cited by 2 publications

References 29 publications

Use of Saponinosomes from Ziziphus spina-christi as Anticancer Drug Carriers

Use of Saponinosomes from Ziziphus spina-christi as Anticancer Drug Carriers

Characterisation of bioactive compounds from leaves of Smilax fluminensis Steud. by gc-MS and LC-DAD-MS, and analysis of its antioxidant and ecotoxicological activities

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