Therapeutic strategies of three-dimensional stem cell spheroids and organoids for tissue repair and regeneration

Kim, Woochan; Gwon, Yonghyun; Park, Sunho; Kim, Hyoseong; Kim, Jangho

doi:10.1016/j.bioactmat.2022.03.039

Cited by 101 publications

(71 citation statements)

References 245 publications

(390 reference statements)

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“…Currently, tissue engineering approaches for treatment of temporomandibular joint disorders (TMD) are a much needed alternative to the limited efficacy of routinely-used clinical treatment modalities ( Acri et al, 2019 ; Tarafder et al, 2016 ). Inducing differentiation of stem cells into chondrocytes within the condylar cartilage is a possible treatment strategy for TMD ( Kim et al, 2023 ; Wadhwa and Kapila, 2008 ). However, biomaterials for cartilage regeneration within the temporomandibular joint (TMJ) is still in the developmental pipeline.…”

Section: Introductionmentioning

confidence: 99%

Superwettable and injectable GelMA-MSC microspheres promote cartilage repair in temporomandibular joints

Yang

Huang²,

Zheng³

et al. 2022

Front. Bioeng. Biotechnol.

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Temporomandibular disorders (TMD) can be treated by promoting cartilage regeneration with biomaterials. However, there are deficiencies in the infiltration function of bone filler biological materials. In this study, stems cells were loaded onto gelatin methacryloyl (GelMA) hydrogel microspheres endowed with superwettable properties and TGF-β sustained-release function, which can quickly infiltrate the irregular surface of the temporomandibular joint (TMJ) bone defect area and accelerate cartilage healing. First, to improve cell adhesion and spreading function, the BMSCs-coated GelMA microspheres were endowed with superwetting property. At the same time, the swelling adsorption characteristics of gelatin microspheres could be used to load recombinant TGF-β within the microspheres, which could in turn promote the chondrogenic differentiation of multi-potent bone marrow mesenchymal stem cells. The SEM imaging demonstrated that BMSCs-coated GelMA microsphere has superwettable and superhydrophilic property, which enabled rapid adaptation to the bone defect surface morphology, which is conducive to tissue repair. Furthermore, the cartilage defect model showed that rBMSCs-coated GelMA microspheres promote temporomandibular joint arthritis repair. In conclusion, our study established that BMSC-coated GelMA microspheres endowed with superwetting properties, can colonize the bone defect repair site better with sustained release of growth factors, thus providing an innovative strategy for promoting cartilage regeneration.

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Section: Introductionmentioning

confidence: 99%

Superwettable and injectable GelMA-MSC microspheres promote cartilage repair in temporomandibular joints

Yang

Huang²,

Zheng³

et al. 2022

Front. Bioeng. Biotechnol.

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“…However, removing them from their endogenous 3D niche, as well as enzymatic passaging, can result in a loss of multipotency, accumulation of chromosomal aberrations within the MSCs genome, dysfunction in DNA repair, replicative senescence, decreased surface markers (e.g., CD105, CD90, CD73) associated with the MSCs’ undifferentiated phenotype [ 94 , 95 , 96 , 97 , 98 ]. Three-dimensional cell culture systems’ ability to bridge the gap between cell-based methods and animal models for studying the repair and replacement of tissues and organs is becoming increasingly apparent [ 99 ].…”

Section: The Immunomodulatory Effects Of Pretreated Mscs and Their Se...mentioning

confidence: 99%

Pretreated Mesenchymal Stem Cells and Their Secretome: Enhanced Immunotherapeutic Strategies

Cheng

et al. 2023

IJMS

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Mesenchymal stem cells (MSCs) with self-renewing, multilineage differentiation and immunomodulatory properties, have been extensively studied in the field of regenerative medicine and proved to have significant therapeutic potential in many different pathological conditions. The role of MSCs mainly depends on their paracrine components, namely secretome. However, the components of MSC-derived secretome are not constant and are affected by the stimulation MSCs are exposed to. Therefore, the content and composition of secretome can be regulated by the pretreatment of MSCs. We summarize the effects of different pretreatments on MSCs and their secretome, focusing on their immunomodulatory properties, in order to provide new insights for the therapeutic application of MSCs and their secretome in inflammatory immune diseases.

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“…For tissue regeneration, obtaining mature organoids is critical to achieve vascularization, reduce unnecessary differentiation after transplantation, and promote functional reconstitution. 81 Intestinal organoids are a relatively mature system among organoid models, which were first applied in regenerative medicine. For example, Cruz-Acuna et al 128 encapsulated hPSCs into a PEG hydrogel matrix, which was set as the carrier, to yield intestinal organoids.…”

Section: Tissue Regeneration Of Organoidsmentioning

confidence: 99%

“…79 This is because the 2D culture systems lack the cell-cell and cell-matrix interactions that require for maintaining and defining in situ phenotypes, thus cannot mimic the cellular functions and signaling pathways present in tissues. 80 In contrast, 3D cell culture systems have properties that help guide specific functions, growth, and processes of stem cells, such as embryogenesis, morphogenesis, and organogenesis, 81 so further exploration of 3D culture systems is needed.…”

Section: Introductionmentioning

confidence: 99%

Stem cells for organoids

et al. 2022

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Organoids are three‐dimensional (3D) cell culture systems that simulate the structures and functions of organs, involving applications in disease modeling, drug screening, and cellular developmental biology. The material matrix in organoids can provide a 3D environment for stem cells to differentiate into different cell types and continuously self‐renew, thereby realizing the in vitro culture of organs, which has received extensive attention in recent years. However, some challenges still exist in organoids, including low maturity, high heterogeneity, and lack of spatiotemporal regulation. Therefore, in this review, we summarized the culturing protocols and various applications of stem cell‐derived organoids and proposed insightful thoughts for engineering stem cells into organoids in view of the current shortcomings, to achieve the further application and clinical translation of stem cells and engineered stem cells in organoid research.

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Therapeutic strategies of three-dimensional stem cell spheroids and organoids for tissue repair and regeneration

Cited by 101 publications

References 245 publications

Superwettable and injectable GelMA-MSC microspheres promote cartilage repair in temporomandibular joints

Superwettable and injectable GelMA-MSC microspheres promote cartilage repair in temporomandibular joints

Pretreated Mesenchymal Stem Cells and Their Secretome: Enhanced Immunotherapeutic Strategies

Stem cells for organoids

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