Thermal ablation of tumor cells with antibody-functionalized single-walled carbon nanotubes

Abstract

Single-walled carbon nanotubes (CNTs) emit heat when they absorb energy from near-infrared (NIR) light. Tissue is relatively transparent to NIR, which suggests that targeting CNTs to tumor cells, followed by noninvasive exposure to NIR light, will ablate tumors within the range of NIR. In this study, we demonstrate the specific binding of antibody-coupled CNTs to tumor cells in vitro, followed by their highly specific ablation with NIR light. Biotinylated polar lipids were used to prepare stable, biocompatible, noncytotoxic CNT dispersions that were then attached to one of two different neutralite avidin-derivatized mAbs directed against either human CD22 or CD25. CD22 ؉ CD25 ؊ Daudi cells bound only CNTs coupled to the anti-CD22 mAb; CD22 ؊ CD25 ؉ activated peripheral blood mononuclear cells bound only to the CNTs coupled to the anti-CD25 mAb. Most importantly, only the specifically targeted cells were killed after exposure to NIR light.immunoconjugates ͉ lymphoma cells ͉ monoclonal antibodies ͉ nanotechnology ͉ near infrared light

Transferrin-functionalized nanoparticles lose their targeting capabilities when a biomolecule corona adsorbs on the surface

“…Success in similar systems by no means implies success in vivo, but if targeting is not observed in relevant fluids, apparent targeting in vivo may be a consequence of other poorly understood phenomena. Indeed, we may generalize these comments, beyond the focus of these experiments, since binding to cells or tissue components (rather than uptake) may be sufficient prior to thermal ablation 34,35 or other therapeutic strategies. Such cases also could benefit from a comparable in situ binding analysis as discussed here.…”

Carbon nanotubes as a novel drug delivery system for anticancer therapy: a review

“…The success of this technique depends heavily on the surgeon's accuracy when inserting the catheter. By using targeted nanotubes and the aforementioned properties of these when exposed to near infra-red (NIR) light, more accurate, reliable thermal ablation is achievable without requiring an incision Chakravarty et al, 2008).…”

Nanotechnology-based approaches in anticancer research

“…2 In the past, there have been numerous experimental studies performed in vitro and in vivo using antibody-functionalized CNTs loaded with chemotherapeutic agents. 2,[77][78][79] It has been suggested that CNTs could be used as nanocarriers for delivering drugs into the body via intravenous routes. 80 Drugs can either attach to the outer surface of CNTs via functional groups or be loaded inside the CNTs.…”