Thermal lens spectrometry in trace metal analysis

Abroskin, A. G.; Belyaeva, T. V.; Филичкина, В. А.; Ivanova, E. K.; Proscurnin, Michael A.; Savostina, V.M.; Barbalat, Yuri A.

doi:10.1039/an9921701957

Cited by 28 publications

(14 citation statements)

References 6 publications

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“…In spite of a somewhat lower detection sensitivity compared to more commonly used lock-in detection schemes in thermal-lens spectrometry, the flexibility of this measurement mode provide a much larger volume of information, making it a powerful tool for solving so not-straightforward problems like studies of complex formation at trace concentrations, transient heat dynamics around absorbing nanoparticles, etc. [70, 72, 73]. …”

Section: Methodsmentioning

confidence: 99%

Ultrasensitive label‐free photothermal imaging, spectral identification, and quantification of cytochrome c in mitochondria, live cells, and solutions

Brusnichkin

Nedosekin

Galanzha

et al. 2010

Journal of Biophotonics

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Light-absorbing endogenous cellular proteins, in particular cytochrome c, are used as intrinsic biomarkers for studies of cell biology and environment impacts. To sense cytochrome c against real biological backgrounds, we combined photothermal (PT) thermal-lens single channel schematic in a back-synchronized measurement mode and a multiplex thermal-lens schematic in a transient high resolution (ca. 350 nm) imaging mode. These multifunctional PT techniques using continuous-wave (cw) Ar+ laser and a nanosecond pulsed optical parametric oscillator in the visible range demonstrated the capability for label-free spectral identification and quantification of trace amounts of cytochrome c in a single mitochondrion alone or within a single live cell. PT imaging data were verified in parallel by molecular targeting and fluorescent imaging of cellular cytochrome c. The detection limit of cytochrome c in a cw mode was 5 × 10−9 mol/L (80 attomols in the signal-generation zone); that is ca. 103 lower than conventional absorption spectroscopy. Pulsed fast PT microscopy provided the detection limit for cytochrome c at the level of 13 zmol (13 × 10−21 mol) in the ultra-small irradiated volumes limited by optical diffraction effects. For the first time, we demonstrate a combination of high resolution PT imaging with PT spectral identification and ultrasensitive quantitative PT characterization of cytochrome c within individual mitochondria in single live cells. A potential of far-field PT microscopy to sub-zeptomol detection thresholds, resolution beyond diffraction limit, PT Raman spectroscopy, and 3D imaging are further highlighted.

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Section: Methodsmentioning

confidence: 99%

Ultrasensitive label‐free photothermal imaging, spectral identification, and quantification of cytochrome c in mitochondria, live cells, and solutions

Brusnichkin

Nedosekin

Galanzha

et al. 2010

Journal of Biophotonics

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“…The dual-beam thermal-lens spectrometer used in this work and the features of its functioning were described previously in detail. [23][24][25][26][27][28][29] Briefly, the used technique is based on recording an excitation-laserinduced (Innova 90-6, Coherent, Palo Alto, CA; wavelength, 514.5 nm; waist diameter, 59.8 6 0.5 lm; power, 1-150 mW) refractive heterogeneity (thermal lens effect 10 ), causing defocusing of a collinear HeNe laser probe beam (SP-106-1, Spectra Physics, Eugene, OR; wavelength, 632.8 nm; diameter, 25.0 6 0.2 lm; power, 4 mW) and hence a reduction in the probe beam intensity at its center as detected by a far-field photodiode (sample-to-detector distance 120 6 0.1 cm) supplied with a KS-11 stained-glass bandpass filter and a 2 mm diameter pinhole (Fig. 1).…”

Section: Methodsmentioning

confidence: 99%

Photothermal Lens Detection of Gold Nanoparticles: Theory and Experiments

et al. 2007

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An approach for mode-mismatched two-beam (pump-probe) photothermal lens detection of multipoint light-absorbing targets in solution (e.g., gold nanoparticles) is developed for continuous-wave intensity-modulated laser-excitation mode. A description of the blooming of the thermooptical element (thermal lens) upon absorption of the excitation laser radiation is based on the summation of individual thermal waves from multiple heat sources. This description makes it possible to estimate the irregularities of the temperature (and, thus, the refractive index) profile for a discrete number of nanoparticles in the irradiated area and a change in the concentration and particle size parameters. Experimental results are in good agreement with theoretical dependences of the photothermal signal on nanoparticle size and concentration and excitation laser power. Calibration plots for particles from 2 to 250 nm show long linear ranges, limits of detection of gold nanoparticles at the level of hundreds of nanoparticles with the current setup, and the photothermal-lens sensitivity coefficient increases as a cubic function of particle size. Further improvements are discussed, including increasing the sensitivity thresholds up to one nanoparticle in the detected volume.

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“…Spectrophotometric limits of detection and quantification and replicability for cobalt and iron in aqueous solutions with 0.04 -0.1 mol dm -3 of Triton X-100 (Table 3) are at the same level as the corresponding values for the existing spectrophotometric procedures. 13,15,18,19,22 In the case of cobalt the procedure for determining cobalt in aqueous solution with Triton X-100 (Procedure 3) has the same limit of detection as the extraction-spectrophotometric procedure (Procedure 2).…”

Section: Thermal-lens Determination Of Cobalt and Iron In The Presencmentioning

confidence: 99%

“…to this parameter is much less significant than the medium nature, which becomes the most important factor. 2,3,[19][20][21] The RSDstab parameter can be used for estimating the background fluctuations in a given solvent; the comparison of ratios of RSDstab to E0 for a pair of solvents (RE, Eq. (6)) could be a good estimation of the expected ratio of LODs (Table 2).…”

Section: Comparison Of Media For Thermal Lensingmentioning

confidence: 99%

The Use of Triton X-100 in Thermal Lensing of Aqueous Solutions

et al. 2000

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Finely dispersed colloidal systems based on surfactants can be successfully used in thermal lens spectrometry: they change thermooptical parameters of water due to disturbance in the structure of hydrogen bonds resulting in an increase in the temperature gradient of refractive index and a decrease in the thermal conductivity. [1][2][3][4] Until now, a number of studies have dealt with surfactants as media modifiers in thermal lensing, but mainly in physicochemical studies. [5][6][7][8][9][10] The aims of this work were (1) to study the effect of an inert nonionic surfactant (Triton X-100) on performance characteristics of thermal lensing of iron(II)-1,10-phenanthroline and cobalt(III)-2-nitroso-1-naphthol as model systems and (2) to use the change in the thermooptical properties of water for the determination of the surfactant itself. Experimental ApparatusThe scheme of the parallel dual-beam thermal lens spectrometer was described in the previous paper. 11 A pump Ar + laser (Innova 90-6, Coherent, Palo Alto, CA 94303, USA) was operated at 514.5 nm; laser power in the cell was 40 mW (chopping rate 0.3 Hz). An SP-106-1 He-Ne laser (Spectra Physics, Eugene, OR 97402, USA; 10 mW, 632.8 nm) was exploited as a probe. Changes in intensity were measured in the central part (2 mm) of the probe beam by a single far-field photodiode detector connected to a DT-2801A ADC-DAC PC board (Data Translation Inc., Marlboro MA, USA; ADC conversion rate 27.5 kHz). The data were gathered and handled in a real-time mode by the software written in-house. 11 Spectrophotometric measurements were made using an SF-46 spectrophotometer (LOMO, St. Petersburg, Russia). Quartz cells (optical pathlength of 10 mm) were used throughout. Each pH value was measured by an EV-74 potentiometer (Gomel', Belarus) with the precision of ±5%. Solution densities were measured with a KLP Urometr GOST 1032-60 areometer kit (Russia). Refractive indices were measured with an RF-22 refractometer (Russia). All the solutions were homogenized prior to the measurements with a Branson 2510 ultrasonic bath (USA). Reagents and solventsThe following reagents were used throughout: analytical-grade ammonium iron(II) sulfate hexahydrate, 1,10-phenanthroline monohydrate, 2-nitroso-1-naphthol, sodium acetate, and acetic acid (99.7+%); chemically pure cobalt(II) sulfate hydrate, hydrochloric acid, and sodium hydroxide; biochemically pure ascorbic acid; a standard phosphate buffer solution, pH 6.9; and Triton X-100 (molecular biology product, 100%; from Sigma, St. Louis MO, USA). Unless otherwise stated, the reagents were from Reakhim (Moscow, Russia). Distilled and doubly distilled water and analytical-grade chloroform were used as solvents. Pre-synthesized chelates of cobalt(III) tris-(2-nitroso-1-naphtholate) 12 and iron(II) tris-(1,10-phenanthrolinate) 13 were prepared according to existing procedures.Characteristics of aqueous solutions of Triton X-100 are summarized in Table 1. The cloud point is not reached for the studied ranges of surfactant concentrations (1.5×10 ...

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Thermal lens spectrometry in trace metal analysis

Cited by 28 publications

References 6 publications

Ultrasensitive label‐free photothermal imaging, spectral identification, and quantification of cytochrome c in mitochondria, live cells, and solutions

Ultrasensitive label‐free photothermal imaging, spectral identification, and quantification of cytochrome c in mitochondria, live cells, and solutions

Photothermal Lens Detection of Gold Nanoparticles: Theory and Experiments

The Use of Triton X-100 in Thermal Lensing of Aqueous Solutions

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