Thermal pre-processing before extraction of polyhydroxyalkanoates for molecular weight quality control

Werker, Alan; Pei, Ruizhe; Kim, Kevin; Moretto, Giulia; Estévez-Alonso, Ángel; Vermeer, Chris M.; Hernandez, Monica Arcos; Dijkstra, Jelmer; Vries, Erik F. J. de

doi:10.1016/j.polymdegradstab.2023.110277

Cited by 8 publications

(3 citation statements)

References 46 publications

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“… 100% 95 ± 2 - Thermal treatment of dry PHA-rich biomass lowers PHA molecular weight predictably. [ 186 ] Microbial sludge (food pulp waste feed) 60–100 L Biomass dried and treated with 0.3 M NaOH at 30 o C for 300 min 100 99 - Standard protocol with CHCl 3 has recovery of 81–83% recovery The purity of the final product increased with intracellular concentration and irrespective of extraction agent. [ 187 ] 60–100 L Biomass dried and treated with 9.0% NaClO at 30 o C for 3.4 h 90 100 - Secondary sedimentation tank (STP-300 KLD) Jawaharlal Nehru University, India 500 mL 2 g sludge biomass 200 mL CHCl 3 at 70 o C, 180 rpm for 72 h with continuous agitation.…”

Section: Advances In Pha Recovery and Purificationmentioning

confidence: 99%

A review on microbes mediated resource recovery and bioplastic (polyhydroxyalkanoates) production from wastewater

Ahuja,

Singh,

Mahata

et al. 2024

Microb Cell Fact

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Background Plastic is widely utilized in packaging, frameworks, and as coverings material. Its overconsumption and slow degradation, pose threats to ecosystems due to its toxic effects. While polyhydroxyalkanoates (PHA) offer a sustainable alternative to petroleum-based plastics, their production costs present significant obstacles to global adoption. On the other side, a multitude of household and industrial activities generate substantial volumes of wastewater containing both organic and inorganic contaminants. This not only poses a threat to ecosystems but also presents opportunities to get benefits from the circular economy. Main body of abstract Production of bioplastics may be improved by using the nutrients and minerals in wastewater as a feedstock for microbial fermentation. Strategies like feast-famine culture, mixed-consortia culture, and integrated processes have been developed for PHA production from highly polluted wastewater with high organic loads. Various process parameters like organic loading rate, organic content (volatile fatty acids), dissolved oxygen, operating pH, and temperature also have critical roles in PHA accumulation in microbial biomass. Research advances are also going on in downstream and recovery of PHA utilizing a combination of physical and chemical (halogenated solvents, surfactants, green solvents) methods. This review highlights recent developments in upcycling wastewater resources into PHA, encompassing various production strategies, downstream processing methodologies, and techno-economic analyses. Short conclusion Organic carbon and nitrogen present in wastewater offer a promising, cost-effective source for producing bioplastic. Previous attempts have focused on enhancing productivity through optimizing culture systems and growth conditions. However, despite technological progress, significant challenges persist, such as low productivity, intricate downstream processing, scalability issues, and the properties of resulting PHA. Graphical abstract

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Section: Advances In Pha Recovery and Purificationmentioning

confidence: 99%

A review on microbes mediated resource recovery and bioplastic (polyhydroxyalkanoates) production from wastewater

Ahuja,

Singh,

Mahata

et al. 2024

Microb Cell Fact

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“…11 However, due to evident safety concerns, research has been increasingly focused on exploring alternative approaches to extracting PHA in recent years. Extraction of polymers from MMC has been reported 3,12,13 to be more challenging than from pure cultured biomass due to the stronger and more complex extracellular biomass matrix of PHA-accumulating microorganism consortia. Therefore, it represents a litmus test to evaluate the effectiveness of new extraction procedures.…”

Section: ■ Introductionmentioning

confidence: 99%

“…As far as the separation of the polymer by its dissolution, opting for the most environmentally friendly solvent allows for a considerable decrease in the overall environmental footprint. Then, various safe liquids were tested including dimethylcarbonate, − 1-butanol, , 1,2-methyl tetrahydrofuran and cyrene, ethyl acetate (EA), methyl isobutyl ketone, 2-butanol, and acetone . The other procedure for the polymer recovery is based on chemical solubilization of non-PHA cell matrix (NPCM) with strong oxidant, typically NaClO and H 2 O 2 , or bases, mainly NaOH water solution, as well as enzymatic digestion. , However, the use of sodium hypochlorite also presents drawbacks due to the toxicity of potentially formed halogenated compounds and its difficult removal from the polymer .…”

Section: Introductionmentioning

confidence: 99%

Chlorine-free Extractions of Mixed-Culture Polyhydroxyalkanoates Produced from Fermented Sewage Sludge at Pilot Scale

Salvatori,

Alfano,

Martinelli

et al. 2023

Ind. Eng. Chem. Res.

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In this study, various conventional and innovative methods were investigated for the recovery of polyhydroxyalkanoates (PHA) from a single batch of biomass produced at a pilot scale from mixed microbial cultures (MMCs) and fermented sewage sludge as a feedstock. Sustainable chlorine-free methods using NaOH and/or H 2 O 2 , as well as extraction in nontoxic ethyl acetate, were analyzed. Interestingly, the combined treatment of biomass with NaOH and H 2 O 2 solutions demonstrated good recovery (70 wt %) and high purity (92 wt %) of the polymer in small-scale trials. Moreover, when the coupled treatment was performed on a larger biomass quantity (approximately 200 g), it achieved high purity and recovery yield (93 and 88 wt %, respectively), indicating the feasibility of this extraction method on a larger scale.

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An innovative and sustainable process for producing poly(3-hydroxybutyrate-co-3-hydroxyvalerate): simulating volatile fatty acid role and biodegradability

Amabile

Abate

Crescenzo

et al. 2023

Chemical Engineering Journal

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Thermal pre-processing before extraction of polyhydroxyalkanoates for molecular weight quality control

Cited by 8 publications

References 46 publications

A review on microbes mediated resource recovery and bioplastic (polyhydroxyalkanoates) production from wastewater

A review on microbes mediated resource recovery and bioplastic (polyhydroxyalkanoates) production from wastewater

Chlorine-free Extractions of Mixed-Culture Polyhydroxyalkanoates Produced from Fermented Sewage Sludge at Pilot Scale

An innovative and sustainable process for producing poly(3-hydroxybutyrate-co-3-hydroxyvalerate): simulating volatile fatty acid role and biodegradability

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