Thermal Protein Denaturation and Protein Aggregation in Cells Made Thermotolerant by Various Chemicals: Role of Heat Shock Proteins

Kampinga, Harm H.; Brunsting, Jeanette F.; Stege, G. J. J.; Burgman, Paul; Konings, Antonius W.T.

doi:10.1006/excr.1995.1262

Cited by 115 publications

(71 citation statements)

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“…26 Like other conventional treatment modalities, the therapeutic effects of hyperthermia can be limited by intrinsic cellular resistance. 14,15 The incidence of local failure in patients treated with combinations of hyperthermia and radiation 3,5 emphasizes the need to better understand the molecular basis of heat resistance and to develop better therapeutics against this class of tumor cell. MCF-7 cells are resistant to both radiation and heat treatment, 30,31 and carry several molecular defects associated with apoptotic signaling.…”

Section: Discussionmentioning

confidence: 99%

“…Given the relapse rate following thermoradiotherapy for LRBC and the fact that cells can be intrinsically resistant to heat, 14,15 we studied breast cancer cell lines (MCF-7 and T47D) that were resistant to heat-induced cell death. Previous studies in our laboratory demonstrated a 50-to 800-fold increase in reporter gene expression following heat treatment (30 minutes at 431C) of a panel of human breast cancer cell lines infected with a recombinant adenovirus (Ad.70b.bgal) in which a b-galactosidase reporter gene was placed under the control of the wild-type hsp70b gene promoter.…”

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Heat-directed suicide gene therapy for breast cancer

et al. 2003

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Adjuvant hyperthermia can improve treatment outcome for locally recurrent breast cancer (LRBC). Previously, we demonstrated that infection of human breast cancer cells with a recombinant adenovirus expressing b-galactosidase from the human hsp70b gene promoter (Ad.70b.bgal) results in 50-to 800-fold increases in reporter gene expression following heat treatment (30 minutes at 431C). Here, we describe a heat-directed suicide gene therapy strategy based on an adenoviral vector (Ad.70b.CDTK) in which expression of the dual prodrug-activating E. coli cytosine deaminase/herpes simplex virus thymidine kinase (CDTK) fusion gene is under the control of the hsp70b promoter. Treatment of T47D and MCF-7 breast cancer cells with mild hyperthermia (431C/30 minutes) and prodrugs (100 mg/ml 5-fluorocytosine and 10 mg/ml ganciclovir) following infection with Ad.70b.CDTK (10-100 PFU/ cell) resulted in 30-to 60-fold decreases in clonogenic survival relative to control cultures treated with heat or prodrugs alone. Clonogenic survival declined even further (up to 240-fold) following heat treatment at 41.51C for 120 minutes. A decreased clonogenic survival was accompanied by tumor cell apoptosis. These results demonstrate that this combined treatment strategy can be highly effective against heat-and radiation-resistant breast tumor cells and supports the continued development of heat-directed CDTK suicide gene therapy strategies for LRBC.

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Section: Discussionmentioning

confidence: 99%

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Heat-directed suicide gene therapy for breast cancer

et al. 2003

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“…Protein stability and function are also affected by changes in temperature. Heat-induced alterations in enzyme activity, or even enzyme inactivation, translate into metabolic imbalance (Kampinga et al, 1995;Boston et al, 1996). Moreover, membrane and protein damage trigger the production of reactive oxygen species, which lead in turn to heat-induced oxidative stress (Larkindale and Knight, 2002;Larkindale et al, 2005a,b).…”

Section: Heat-induced Damage At the Cellular Levelmentioning

confidence: 99%

Molecular responses to thermal stress in woody plants

et al. 2005

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Stress caused by high and low temperatures has been much less studied in woody plants than in herbaceous ones. The cold response shows specific traits in woody plants. Cold acclimation associated to winter dormancy develops in two sequential steps: the first is triggered by photoperiod shortening while the second requires temperatures to fall. The involvement of ABA in cold acclimation is beyond question, yet the roles played by this hormone are far better understood in herbaceous plants. The induction of protective proteins and changes in both glycan metabolism and circadian clock functioning are noteworthy among the molecular responses of woody species to low temperatures. Both herbaceous and woody plants seem to respond similarly to heat stress, with heat shock proteins (HSPs) dominating protein synthesis profiles. Certain HSPs are also induced in response to low temperatures, and recent data suggest that they are involved in cold acclimation.Key words: cold acclimation, heat stress, dormancy, HSP, ABA. Resumen Respuestas moleculares de las plantas leñosas al estrés térmicoEl estrés que producen las altas y las bajas temperaturas ha sido menos estudiado en las plantas leñosas que en las herbáceas. La respuesta al frío en las especies leñosas presenta características propias. La aclimatación al frío asociada a la dormancia invernal se desarrolla en dos etapas secuenciales: la primera es inducida por el acortamiento del fotoperiodo, y la segunda requiere una disminución de las temperaturas. Se ha comprobado una implicación del ABA en este proceso, sin embargo su papel es menos claro que el que tiene en la aclimatación de las plantas herbáceas. Entre las respuestas moleculares de los árboles al frío se deben destacar la inducción de proteínas protectoras, los cambios en el metabolismo de los glúcidos y la alteración en el funcionamiento del reloj circadiano. El comportamiento de las plantas leñosas frente al calor no parece tener peculiaridades específicas, siendo la síntesis de las proteínas de choque térmico (HSPs) la respuesta más significativa a nivel molecular. Algunas HSPs también se inducen en respuesta a las bajas temperaturas, y datos recientes sugieren que pueden estar implicadas en la aclimatación al frío.Palabras clave: aclimatación al frío, choque de calor, dormancia, HSP, ABA.

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“…Heat alters membrane ßuidity and permeability (Alfonso et al 2001, Sangwan et al 2002, inßuences enzyme activities (Vierling 1991, Kampinga et al 1995, induces production of active oxygen species (Suzuki and Mittler 2006), reduces photosynthesis and carbon gain (Way and Sage 2008), and modiÞes growth and reproduction (Cheikh andJones 1994, Zinn et al 2010). The responses of plants to heat stress at multiple levels reßect the adjustments of plants trying to adapt, survive, and develop in stressed environments (Garrett et al 2006).…”

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Transient Heat Stress Compromises the Resistance of Wheat (Poales: Poaceae) Seedlings to Hessian Fly (Diptera: Cecidomyiidae) Infestation

et al. 2014

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Heat stress exerts a profound impact on the resistance of plants to parasites. In this research, we investigated the impact of an acute transient heat stress on the resistance of the wheat line ÔMolly,Õ which contains the R gene H13, to an avirulent Hessian ßy (Mayetiola destructor (Say)) population. We found that a signiÞcant portion of Molly seedlings stressed at 40ЊC for 6 h during or after the initial Hessian ßy larval attack became susceptible to otherwise avirulent insects, whereas unstressed control plants remained 100% resistant. SpeciÞcally, 77.8, 73.3, 83.3, and 46.7% of plants heat stressed at 0, 6, 12, and 24 h, respectively, after the initial larval attack became susceptible. Biochemical analysis revealed that heat stress caused a transient decrease in 12-oxo-phytodienoic acid, but an increase in salicylic acid accumulation in Molly plants. The change in phytohormones after heat stress and Hessian ßy infestation was not observed in ÔNewton,Õ a near-isogenic but Hessian ßy susceptible wheat line. Instead, heat stress caused a relatively prolonged reduction in palmitoleic acid. The role of phytohormones in heat-induced loss of wheat resistance was discussed.

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Thermal Protein Denaturation and Protein Aggregation in Cells Made Thermotolerant by Various Chemicals: Role of Heat Shock Proteins

Cited by 115 publications

References 0 publications

Heat-directed suicide gene therapy for breast cancer

Heat-directed suicide gene therapy for breast cancer

Molecular responses to thermal stress in woody plants

Transient Heat Stress Compromises the Resistance of Wheat (Poales: Poaceae) Seedlings to Hessian Fly (Diptera: Cecidomyiidae) Infestation

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