2016
DOI: 10.1242/jeb.150698
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Thermal sensitivity and phenotypic plasticity of cardiac mitochondrial metabolism in European perch, Perca fluviatilis

Abstract: Cellular and mitochondrial metabolic capacity of the heart has been suggested to limit performance of fish at warm temperatures. We investigated this hypothesis by studying the effects of acute temperature increases (16, 23, 30, 32.5 and 36°C) on the thermal sensitivity of 10 key enzymes governing cardiac oxidative and glycolytic metabolism in two populations of European perch (Perca fluviatilis) field-acclimated to 15.5 and 22.5°C, as well as the effects of acclimation on cardiac lipid composition. In both p… Show more

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Cited by 51 publications
(51 citation statements)
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“…Total protein concentration was assessed using a BCA protein assay kit at 562 nm with bovine serum albumin (BSA) as the standard. CS activity was determined from the rate of appearance of reduced DTNB (5,5′-dithiobis [2-nitrobenzoic]), which was monitored with a spectrophotometer at 412 nm (Ekstrom et al, 2017 ). For the CS assay, 10 μL of diluted tissue homogenate (1.0 mg/ ml) was incubated with 140 μL reaction medium (0.1 mM DTNB, 0.2 mM AcetylCoA, 0.15 mM oxaloacetic acid, pH 8.0).…”
Section: Methodsmentioning
confidence: 99%
“…Total protein concentration was assessed using a BCA protein assay kit at 562 nm with bovine serum albumin (BSA) as the standard. CS activity was determined from the rate of appearance of reduced DTNB (5,5′-dithiobis [2-nitrobenzoic]), which was monitored with a spectrophotometer at 412 nm (Ekstrom et al, 2017 ). For the CS assay, 10 μL of diluted tissue homogenate (1.0 mg/ ml) was incubated with 140 μL reaction medium (0.1 mM DTNB, 0.2 mM AcetylCoA, 0.15 mM oxaloacetic acid, pH 8.0).…”
Section: Methodsmentioning
confidence: 99%
“…To this extent, mitochondria are likely to be a key player in defining the environmental conditions that an organism can tolerate and in which it can thrive, owing to the predominant role they play in aerobic metabolism and bioenergetics [30,31]. Mitochondrial efficiency and respiration rates can be impaired under acute environmental stress owing to the failure of metabolic enzymes and mitochondrial complexes that govern substrate oxidation, electron transport and oxidative phosphorylation [32,33]. Given enough time, however, alterations in mitochondrial properties (e.g.…”
Section: Introductionmentioning
confidence: 99%
“…If an insufficient supply of oxygen to tissue does indeed limit upper thermal tolerance in fishes, arterial oxygen convection (i.e., the product of cardiac output and arterial oxygen content) should become constrained as fish are warmed, while increased oxygen-carrying capacity of the blood should be associated with higher thermal tolerance. A seemingly universal finding in fishes is that cardiac function (i.e., cardiac output, heart rate, and mitochondrial metabolic capacity) peaks and then declines at temperatures lower than CT max (Farrell et al 1996;Gollock et al 2006;Hilton et al 2010;Mendonça and Gamperl 2010;Eliason et al 2013;Iftikar and Hickey 2013;Ferreira et al 2014;Muñoz et al 2014;Penney et al 2014;Rodnick et al 2014;Ekström et al 2017). Conversely, tests of the relationship between CT max and oxygencarrying capacity have yielded mixed results.…”
Section: Introductionmentioning
confidence: 99%