In the present work, chemically synthesis compared to Biosynthesized CeO 2 NP S using aqueous extract of M. charantia (15 mL) concentrations. Influence of the synthesized products were characterized by Ultra violet-Vis spectroscopy, X-ray diffraction, Fourier transform infrared, Scanning electron microscopy and, UV-visible spectrometry study revealed surface plasmon resonance at 276 nm, The XRD patterns show the cubic phase and average particle sizes are 15 nm, The functional groups and band area of the samples were established by FT-IR spectroscopy the presence of both leaf extract and CeO 2 NPs. The surface morphologies analysis has been hexagonal quartzite structure confirmed from SEM. The present Bio-CeO 2 NPs nanoparticles suggests that have the great potential applications on various industrial and medical fields of research