Thermal transients excite neurons through universal intramembrane mechano-electrical effects

Plaksin, Michael; Kimmel, Eitan; Shoham, Sharon

doi:10.1101/111724

Cited by 15 publications

(15 citation statements)

References 49 publications

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“…To our knowledge, experimental results of the depolarization amplitude responses function of dT/dt have not been previously reported. A recent theoretical work has highlighted the importance of the membrane physical dimension variations induced by the laser pulse, confirming a capacitive current sensitive to the rate dT/dt [35]. However, membrane potential equations presented in this work suggest that the whole cell depolarization depends only on the temperature increase ΔT, not the rate dT/dt.…”

Section: Discussionmentioning

confidence: 40%

Millisecond infrared laser pulses depolarize and elicit action potentials on in-vitro dorsal root ganglion neurons

Paris¹,

Marc²,

Charlot³

et al. 2017

Biomed. Opt. Express

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This work focuses on the optical stimulation of dorsal root ganglion (DRG) neurons through infrared laser light stimulation. We show that a few millisecond laser pulse at 1875 nm induces a membrane depolarization, which was observed by the patch-clamp technique. This stimulation led to action potentials firing on a minority of neurons beyond an energy threshold. A depolarization without action potential was observed for the majority of DRG neurons, even beyond the action potential energy threshold. The use of ruthenium red, a thermal channel blocker, stops the action potential generation, but has no effects on membrane depolarization. Local temperature measurements reveal that the depolarization amplitude is sensitive to the amplitude of the temperature rise as well as to the time rate of change of temperature, but in a way which may not fully follow a photothermal capacitive mechanism, suggesting that more complex mechanisms are involved.

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Section: Discussionmentioning

confidence: 40%

Millisecond infrared laser pulses depolarize and elicit action potentials on in-vitro dorsal root ganglion neurons

Paris¹,

Marc²,

Charlot³

et al. 2017

Biomed. Opt. Express

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show abstract

“…These coupled equations describe the relation between the mobile (Q) and fixed (

) membrane charge densities and the potential drops of the bulk regions (

). We used the notation according to Plaksin and co-workers ( Plaksin et al, 2017a , Plaksin et al, 2017b ).

2

2

…”

Section: Methodsmentioning

confidence: 99%

A label-free approach to detect ligand binding to cell surface proteins in real time

Burtscher

Hoťka

et al. 2018

eLife

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Electrophysiological recordings allow for monitoring the operation of proteins with high temporal resolution down to the single molecule level. This technique has been exploited to track either ion flow arising from channel opening or the synchronized movement of charged residues and/or ions within the membrane electric field. Here, we describe a novel type of current by using the serotonin transporter (SERT) as a model. We examined transient currents elicited on rapid application of specific SERT inhibitors. Our analysis shows that these currents originate from ligand binding and not from a long-range conformational change. The Gouy-Chapman model predicts that adsorption of charged ligands to surface proteins must produce displacement currents and related apparent changes in membrane capacitance. Here we verified these predictions with SERT. Our observations demonstrate that ligand binding to a protein can be monitored in real time and in a label-free manner by recording the membrane capacitance.

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“…Additional consequences of the larger and more compartmentalized intracellular Na + fluxes also merit consideration. Perhaps, infrared induced electro-mechanical effects ( Plaksin et al, 2018 ) may provide opportunities for remote neuronal excitation ( Shapiro et al, 2012 ) without reliance on substantial ionic currents. In the case of astrocytes, new tools are clearly needed to remotely manipulate physiological levels of activity.…”

Section: Discussionmentioning

confidence: 99%

Transient, Consequential Increases in Extracellular Potassium Ions Accompany Channelrhodopsin2 Excitation

et al. 2019

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Thermal transients excite neurons through universal intramembrane mechano-electrical effects

Abstract: Modern advances in neurotechnology rely

Cited by 15 publications

References 49 publications

Millisecond infrared laser pulses depolarize and elicit action potentials on in-vitro dorsal root ganglion neurons

Millisecond infrared laser pulses depolarize and elicit action potentials on in-vitro dorsal root ganglion neurons

A label-free approach to detect ligand binding to cell surface proteins in real time

Transient, Consequential Increases in Extracellular Potassium Ions Accompany Channelrhodopsin2 Excitation

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