Thermal unfolding and refolding of lysozyme in deep eutectic solvents and their aqueous dilutions

Esquembre, Rocío; Sanz, Jesús M.; Wall, J. Gerard; Monte, Francisco del; Mateo, C. Reyes; Ferrer, M. Luisa

doi:10.1039/c3cp44299c

Cited by 125 publications

(137 citation statements)

References 48 publications

Supporting

Mentioning

123

Contrasting

Order By: Relevance

“…Several factors including hydrophobicity, solvent polarity and hydrogen bond characteristics of ILs can affect separately or together the protein stability and activity (Weingartner et al 2012). Similarly, the concentration of DESs in an aqueous solution had significant effect on the unfolding and refolding process of the enzyme, which could be determined by spectroscopy technologies such as intrinsic fluorescence and CD spectroscopy (Esquembre et al 2013). The two methods can effectively estimate the folded degree of proteins compared to the native-like secondary and tertiary structures.…”

Section: Influence Of Dess On Biocatalysismentioning

confidence: 99%

“…A redshift of the fluorescence emission spectra of tryptophan residues in lysozyme was observed when tryptophan side chain was exposed to a polar surrounding of water molecules. In neat ChCl/U and ChCl/Gly solution, the emission maximum of lysozyme shifted from 335 nm (observed in buffer) to 332 and 329 nm, respectively (Esquembre et al 2013). However, higher concentration of DESs can cause the irreversible unfolding of lysozyme at high temperatures.…”

Section: Influence Of Dess On Biocatalysismentioning

confidence: 99%

See 1 more Smart Citation

Recent progress on deep eutectic solvents in biocatalysis

Zheng

Zong

et al. 2017

Bioresour. Bioprocess.

297

161

View full text Add to dashboard Cite

Deep eutectic solvents (DESs) are eutectic mixtures of salts and hydrogen bond donors with melting points low enough to be used as solvents. DESs have proved to be a good alternative to traditional organic solvents and ionic liquids (ILs) in many biocatalytic processes. Apart from the benign characteristics similar to those of ILs (e.g., low volatility, low inflammability and low melting point), DESs have their unique merits of easy preparation and low cost owing to their renewable and available raw materials. To better apply such solvents in green and sustainable chemistry, this review firstly describes some basic properties, mainly the toxicity and biodegradability of DESs. Secondly, it presents several valuable applications of DES as solvent/co-solvent in biocatalytic reactions, such as lipase-catalyzed transesterification and ester hydrolysis reactions. The roles, serving as extractive reagent for an enzymatic product and pretreatment solvent of enzymatic biomass hydrolysis, are also discussed. Further understanding how DESs affect biocatalytic reaction will facilitate the design of novel solvents and contribute to the discovery of new reactions in these solvents.

show abstract

Section: Influence Of Dess On Biocatalysismentioning

confidence: 99%

Section: Influence Of Dess On Biocatalysismentioning

confidence: 99%

Recent progress on deep eutectic solvents in biocatalysis

Zheng

Zong

et al. 2017

Bioresour. Bioprocess.

297

161

View full text Add to dashboard Cite

show abstract

“…The eutectic mixture melts as low as 12°C. Lysozyme dissolved in DES retains enzyme activity (32). Because DES enhances the solubility of biomolecules, it has been used for preclinical studies (33).…”

Section: Introductionmentioning

confidence: 99%

Structure, stability and behaviour of nucleic acids in ionic liquids

Tateishi‐Karimata

2014

Nucleic Acids Res

116

View full text Add to dashboard Cite

Nucleic acids have become a powerful tool in nanotechnology because of their conformational polymorphism. However, lack of a medium in which nucleic acid structures exhibit long-term stability has been a bottleneck. Ionic liquids (ILs) are potential solvents in the nanotechnology field. Hydrated ILs, such as choline dihydrogen phosphate (choline dhp) and deep eutectic solvent (DES) prepared from choline chloride and urea, are ‘green’ solvents that ensure long-term stability of biomolecules. An understanding of the behaviour of nucleic acids in hydrated ILs is necessary for developing DNA materials. We here review current knowledge about the structures and stabilities of nucleic acids in choline dhp and DES. Interestingly, in choline dhp, A–T base pairs are more stable than G–C base pairs, the reverse of the situation in buffered NaCl solution. Moreover, DNA triplex formation is markedly stabilized in hydrated ILs compared with aqueous solution. In choline dhp, the stability of Hoogsteen base pairs is comparable to that of Watson–Crick base pairs. Moreover, the parallel form of the G-quadruplex is stabilized in DES compared with aqueous solution. The behaviours of various DNA molecules in ILs detailed here should be useful for designing oligonucleotides for the development of nanomaterials and nanodevices.

show abstract

“…At 20°C, the highest fluorescence intensity was observed and the k max was located at 340.6 nm with k ex = 295 (Fig. 1A), suggesting that the most tryptophan residues were located in relatively hydrophobic environments in apo-CP43 (Lakowicz and Masters 2008;Esquembre et al 2013;Drake and Hoops 2014). However, the k max is not obviously shifted with the elevated temperature.…”

Section: Resultsmentioning

confidence: 97%

“…The ratio increases sharply from 68 to 71°C and then slowly with the increasing temperature. The synchronous fluorescence spectra (Dk = 15) indicated that there existed the change in the hydrophobicity around the tyrosine residues and it is gradually increasing with the elevated temperature (Samuel et al 2000;Esquembre et al 2013; Drake and Hoops 2014). These results above indicated that high-temperature treatment disrupted the substructures around both of the tryptophan and tyrosine residues in apo-CP43, especially above 40°C, and the heat-induced structural changes were different in the polarity and hydrophobicity around tryptophan and tyrosine residues.…”

Section: Resultsmentioning

confidence: 99%

Heat-induced unfolding of apo-CP43 studied by fluorescence spectroscopy and CD spectroscopy

Xiao

Yang

et al. 2015

Photosynth Res

View full text Add to dashboard Cite

CP43 is a chlorophyll-binding protein, which acts as a conduit for the excitation energy transfer. The thermal stability of apo-CP43 was studied by intrinsic fluorescence, exogenous ANS fluorescence, and circular dichroism spectroscopy. Under heat treatment, the structure of apo-CP43 changed and existed transition state occurred between 56 and 62 °C by the intrinsic, exogenous ANS fluorescence and the analysis of hydrophobicity. Besides, the isosbestic point of the sigmoidal curve was 58.10 ± 1.02 °C by calculating α-helix transition and the Tm was 56.45 ± 0.52 and 55.59 ± 0.68 °C by calculating the unfolded fraction of tryptophan and tyrosine fluorescence, respectively. During the process of unfolding, the hydrophobic structure of C-terminal segment firstly started to expose at 40 °C, and then the hydrophobic cluster adjacent to the N-terminal segment also gradually exposed to hydrophilic environment with increasing temperature. Our results indicated that heat treatment, especially above 40 °C, has an important impact on the structural stability of apo-CP43.

show abstract

Thermal unfolding and refolding of lysozyme in deep eutectic solvents and their aqueous dilutions

Cited by 125 publications

References 48 publications

Recent progress on deep eutectic solvents in biocatalysis

Recent progress on deep eutectic solvents in biocatalysis

Structure, stability and behaviour of nucleic acids in ionic liquids

Heat-induced unfolding of apo-CP43 studied by fluorescence spectroscopy and CD spectroscopy

Contact Info

Product

Resources

About