Gold nanoparticles associated with DNA, RNA, proteins, oligonucleotides, and peptides are useful in therapies and drug delivery. The present article mainatins that gold nanoparticles play a tremendous role in remedying cancer and fatal diseases. A mathematical model is proposed for the two-dimensional motion of the couple stress nanofluid consisting of gold nanoparticles under the application of peristaltic propulsion and electroosmosis mechanisms in an asymmetric microchannel. The effects of radiation with slip boundary have been employed. The governing equations are simplified under the assumptions of low Reynolds number and long wavelength and the Poisson-Boltzmann equation is solved under Debye-Hückel linearization. Analytical solutions for the velocity of fluid motion, nanoparticle temperature, stream function, pressure gradient, are evaluated and analyzed graphically under the effects of various physical parameters. It is notable from the analysis that raising the Brinkman number boosts the nanoparticle temperature and heat transfer coefficient which validate the physical model and analysis. Moreover, it is noticed that sphere-shaped gold