Thermally triggered injectable chitosan/silk fibroin/bioactive glass nanoparticle hydrogels for in-situ bone formation in rat calvarial bone defects

Wu, Jingjing; Zheng, Kai; Huang, Xian-Ju; Liu, Jiaoyan; Liu, Haoming; Boccaccını, Aldo R.; Wan, Ying; Guo, Xiaodong; Shao, Zhang

doi:10.1016/j.actbio.2019.04.023

Cited by 162 publications

(113 citation statements)

References 54 publications

Supporting

Mentioning

113

Contrasting

Order By: Relevance

“…Bioactive glass nanoparticles (BGNs) are attracting increasing attention as building blocks for developing nanocomposites and hybrids, considering their controllable particle size/shape, bioreactivity, and degradation rate that can lead to superior biocompatibility, bioactivity, osteogenic and angiogenic activities [ 13 , 14 ]. In particular, incorporation of BGNs into biopolymer-based hydrogels can enhance the mechanical properties, osteogenic and angiogenic activities [ 15 , 16 ]. For example, Cu-doped BGNs were incorporated into chitosan/silk fibroin based hydrogels, which enhanced osteogenic and angiogenic activities of the hydrogel as well as the in vivo bone regeneration capability [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…In particular, incorporation of BGNs into biopolymer-based hydrogels can enhance the mechanical properties, osteogenic and angiogenic activities [ 15 , 16 ]. For example, Cu-doped BGNs were incorporated into chitosan/silk fibroin based hydrogels, which enhanced osteogenic and angiogenic activities of the hydrogel as well as the in vivo bone regeneration capability [ 16 ]. Moreover, mesoporous BGNs (MBGNs), due to the high specific surface area and porosity, can interact with polymeric matrices and biomolecules to a greater extent in comparison to nonporous BGNs, which may lead to enhanced mechanical reinforcement and biological activities.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hybrid gelatin/oxidized chondroitin sulfate hydrogels incorporating bioactive glass nanoparticles with enhanced mechanical properties, mineralization, and osteogenic differentiation

Lei

Fan

Zhang

et al. 2021

Bioactive Materials

Self Cite

107

View full text Add to dashboard Cite

Biopolymer based hydrogels are characteristic of their biocompatibility and capability of mimicking extracellular matrix structure to support cellular behavior. However, these hydrogels suffer from low mechanical properties, uncontrolled degradation, and insufficient osteogenic activity, which limits their applications in bone regeneration. In this study, we developed hybrid gelatin (Gel)/oxidized chondroitin sulfate (OCS) hydrogels that incorporated mesoporous bioactive glass nanoparticles (MBGNs) as bioactive fillers for bone regeneration. Gel-OCS hydrogels could be self-crosslinked in situ under physiological conditions in the presence of borax. The incorporation of MBGNs enhanced the crosslinking and accelerated the gelation. The gelation time decreased with increasing the concentration of MBGNs added. Incorporation of MBGNs in the hydrogels significantly improved the mechanical properties in terms of enhanced storage modulus and compressive strength. The injectability of the hydrogels was not significantly affected by the MBGN incorporation. Also, the proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells in vitro and rat cranial defect restoration in vivo were significantly promoted by the hydrogels in the presence of MBGNs. The hybrid Gel-OCS/MBGN hydrogels show promising potential as injectable biomaterials or scaffolds for bone regeneration/repair applications given their tunable degradation and gelation behavior as well as favorable mechanical behavior and osteogenic activities.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hybrid gelatin/oxidized chondroitin sulfate hydrogels incorporating bioactive glass nanoparticles with enhanced mechanical properties, mineralization, and osteogenic differentiation

Lei

Fan

Zhang

et al. 2021

Bioactive Materials

Self Cite

107

View full text Add to dashboard Cite

show abstract

“…Therefore, the content of silk fibroin in PVA hydrogel may be one of the factors that affect cell morphology. Although silk fibroin hydrogels are beneficial for stem cell growth and differentiation [20,21], and silk fibroin composite scaffolds are also widely studied, such as silk fibroin/chitosan/graphene oxide scaffolds [22] and silk fibroin/chitosan/bioactive glass nanoparticle scaffolds [23]. These studies indicated that the composition and content of silk fibroin in composite scaffolds play an important role in cell morphology and behavior.…”

Section: Discussionmentioning

confidence: 99%

Structure and properties of PVA/silk fibroin hydrogels and their effects on growth behavior of various cell types

Hou

Wang

Han

et al. 2020

Mater. Res. Express

View full text Add to dashboard Cite

Controllable regulation of cell behavior is one of the most important factors conducive to the restoration of tissue functions. Recently, various strategies have been developed using physical or chemical cues. Although these techniques are effective, the high cost and complex fabrication procedures impede their application. In this study, we used a low cost and simple strategy to fabricate PVA/silk fibroin composite hydrogels using a cyclic freeze-thaw method. With the increase of freezethaw cycles, the pore size of hydrogels decreased, the elastic modulus increased, and the swelling rate decreased. Furthermore, we chose two shapes of model cells, a spindle using bone marrow mesenchymal stem cells and smooth muscle cells, and a round shape using BV2 microglial cells. PVA/ silk fibroin composite hydrogels inhibited the adhesion and proliferation of stem cells and muscle cells and changed their cell shape from spindle to round, maintained the initial round shape of BV2 microglial cells, and promoted the proliferation of BV2 microglial cells. These results demonstrate that PVA/silk fibroin composite hydrogels can be used as a novel hydrogel system to regulate cell behavior.

show abstract

“…This study shows that the elastic modulus of the hybrid scaffold signi cantly increased when compared to CS/GP gel alone (4kPa), after the PLA and Col II were supplemented. Wu Jingjing and others designed and synthesized chitosan/silk broin/nano-hydroxyapatite composite gel by adding nano-hydroxyapatite to signi cantly improve the mechanical properties, and the elastic modulus can reach more than 40 kPa [24]. However, BMSCs still showed desired proliferation and differentiation into chondrocytes in the CS/Col II /PLA/GP gel, which may be related to the lower mechanical properties of the gel.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of a Thermally Triggered Injectable Hydrogel CS/Col /PLA/GP and BMSCs for Cartilage Tissue Engineering

2020

Preprint

View full text Add to dashboard Cite

BackgroundArticular cartilage has limited self-repair ability. Tissue engineering is considered to be one of the most promising therapeutic approaches. Chitosan (CS) based hydrogels are the most widely used scaffolds which still need improvement. The purpose of this study was to investigate the efficacy of a thermally triggered injectable chitosan / type II collagen / polylactic acid / sodium β-glycerophosphate (CS/Col/PLA/GP) hydrogel and bone marrow mesenchymal stem cells (BMSCs) for the treatment of cartilage defects in rabbit knee joints. Material/MethodsThe CS-based hydrogels consisting of CS, Col II, PLA and GP were fabricated by chemical cross-linking method. The gel forming time and elastic modulus of these hydrogels were measured. We tested the viability, proliferation and differentiation of rabbit BMSCs cultured in the hydrogels by fluorescence staining, CCK-8 and PCR method. The hydrogels combined with or without BMSCs were injected into cartilage defects in rabbit knee joints and the materials were collected at 8 weeks after surgery. The repair effect of cartilage defects was evaluated based on gross observation, HE, safranin O and immunohistochemical staining. ResultsThe CS/Col/PLA/GP hydrogel was liquid at room temperature and gelled after 7.5±0.41min at 37°C. CS/Col /PLA/GP hydrogel had a modulus of 8.90 ± 0.12 kPa while CS/GP and CS/Col/GP hydrogels had the modulus of 4.07 ± 0.24 kPa and 4.93 ± 0.09 kPa. The results of Live/Dead cell viability assay reveal that most of BMSCs remained alive in the hydrogels. CCK-8 assay shows that the number of cells in CS/Col /PLA/GP hydrogel was significantly higher in comparison to the other groups on days 2 and 3 of cell culture (p<0.05). Aggrecan mRNA expression in the CS/Col /PLA/GP gel was the highest (p<0.05). Sox9 mRNA expression in the CS/Col /GP group was the highest, in which CS/Col /PLA/GP hydrogel was higher than the CS/GP hydrogel(p<0.05). Furthermore, CS/Col/PLA/GP and CS/Col /GP hydrogels showed higher COL2A1 mRNA expression in comparison to CS/GP constructs (p<0.05). In vivo studies showed that approximately 90% of the cartilage defects of rabbits treated by the hydrogel and BMSCs were repaired with hyaline-like tissue without obvious inflammation response. HE, safranin O, and immunohistochemical staining showed that the hyaline like cartilage was formed in cartilage defects, and the collagen content in the new generated cartilage was similar to the normal cartilage. The neocartilage was thinner than the surrounding normal cartilage, but it exhibited integration with adjacent healthy tissue. The abundant well-defined chondrocytes were aligned in several apparent chondrocyte clusters in the new generated cartilage.ConclusionsThe thermo-sensitive injectable CS/Col/PLA/GP composite hydrogel has better ability to promote survive, proliferation and chondrogenic differentiation of seeded BMSCs as compared against CS/Col/GP and CS/GP hydrogels. Combined with BMSCs to repair cartilage defects of rabbit knee joints, they can effectively reduce the cartilage defect area, and the new generated cartilage is comparable to normal cartilage structure. In addition, abundant availability and simple fabrication process also make CS/Col/PLA/GP composite hydrogel a suitable candidate scaffold in cartilage tissue engineering.

show abstract

Thermally triggered injectable chitosan/silk fibroin/bioactive glass nanoparticle hydrogels for in-situ bone formation in rat calvarial bone defects

Cited by 162 publications

References 54 publications

Hybrid gelatin/oxidized chondroitin sulfate hydrogels incorporating bioactive glass nanoparticles with enhanced mechanical properties, mineralization, and osteogenic differentiation

Hybrid gelatin/oxidized chondroitin sulfate hydrogels incorporating bioactive glass nanoparticles with enhanced mechanical properties, mineralization, and osteogenic differentiation

Structure and properties of PVA/silk fibroin hydrogels and their effects on growth behavior of various cell types

Efficacy of a Thermally Triggered Injectable Hydrogel CS/Col /PLA/GP and BMSCs for Cartilage Tissue Engineering

Contact Info

Product

Resources

About