Thermodynamic analysis of isoproterenol binding to β-adrenoceptors in rat lung membranes

Thermodynamic analysis of ligands at cholecystokinin CCK2 receptors in rat cerebral cortex

“…Thermodynamic discrimination of agonists and antagonists, at some receptors, has been shown to be dependent on the presence of high‐affinity agonist binding such that it is ablated when high‐affinity agonist binding is prevented by including guanine nucleotides in the assay buffer (for example Contreras et al ., 1986 ; Fraeyman et al ., 1994 ; Lorenzen et al ., 2000 ). In previous studies we have shown that high affinity agonist binding at the CCK 2 receptor is consistent with ternary complex formation between agonist, receptor and G‐protein ( Harper et al ., 1996b ), according to the model proposed by Kent et al (1980) .…”