Thermodynamic and conformational changes of protein toward interaction with nanoparticles: a spectroscopic overview

Zeinabad, Hojjat Alizadeh; Kachooei, Ehsan; Saboury, Ali Akbar; Kostova, Irena; Attar, Farideh; Vaezzadeh, Mahsa; Falahati, Mojtaba

doi:10.1039/c6ra16422f

Cited by 94 publications

(44 citation statements)

References 150 publications

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“…ΔH°.0 and ΔS°.0 exhibit hydrophobic interactions, ΔH°,0 and ΔS°,0 show hydrogen bonds and van der Waals interactions, and ΔH°,0 and ΔS°.0 represent electrostatic interactions. 40 Negative values of ΔH° (−53.21 kJ/mol) and ΔS° (−42.44 kJ/mol) showed that the NP-induced conformational changes of protein is induced by an enthalpy-driven manner. 40 Likewise, ΔH°,0 and ΔS°,0 are characteristics of hydrogen bonds and van der Waals interactions in aqueous solution.…”

Section: H Rt S Rmentioning

confidence: 96%

“…40 Negative values of ΔH° (−53.21 kJ/mol) and ΔS° (−42.44 kJ/mol) showed that the NP-induced conformational changes of protein is induced by an enthalpy-driven manner. 40 Likewise, ΔH°,0 and ΔS°,0 are characteristics of hydrogen bonds and van der Waals interactions in aqueous solution. Due to the large surface-to-volume-ratio of NPs, water molecules are substantially adsorbed on their surface and can mediate the establishment of hydrogen bonds between NP and protein.…”

Section: H Rt S Rmentioning

confidence: 96%

“…Journal of Nanomedicine downloaded from https://www.dovepress.com/ by 44.224.250.200 on 17-Jul-2020 For personal use only.three different temperatures (298, 310, and 315 K) to reveal the mode of fluorescence quenching by using the Stern-Volmer equation (Equation 1):40,41 …”

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confidence: 99%

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Albumin binding, anticancer and antibacterial properties of synthesized zero valent iron nanoparticles

Anbouhi¹,

Esfidvajani²,

Nemati³

et al. 2018

IJN

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BackgroundNanoparticles (NPs) have been emerging as potential players in modern medicine with clinical applications ranging from therapeutic purposes to antimicrobial agents. However, before applications in medical agents, some in vitro studies should be done to explore their biological responses.AimIn this study, protein binding, anticancer and antibacterial activates of zero valent iron (ZVFe) were explored.Materials and methodsZVFe nanoparticles were synthesized and fully characterized by X-ray diffraction, field-emission scanning electron microscope, and dynamic light scattering analyses. Afterward, the interaction of ZVFe NPs with human serum albumin (HSA) was examined using a range of techniques including intrinsic fluorescence, circular dichroism, and UV–visible spectroscopic methods. Molecular docking study was run to determine the kind of interaction between ZVFe NPs and HSA. The anticancer influence of ZVFe NPs on SH-SY5Y was examined by MTT and flow cytometry analysis, whereas human white blood cells were used as the control cell. Also, the antibacterial effect of ZVFe NPs was examined on Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), and Staphylococcus aureus (ATCC 25923).ResultsX-ray diffraction, transmission electron microscope, and dynamic light scattering analyses verified the synthesis of ZVFe NPs in a nanosized diameter. Fluorescence spectroscopy analysis showed that ZVFe NPs spontaneously formed a complex with HSA through hydrogen bonds and van der Waals interactions. Also, circular dichroism spectroscopy study revealed that ZVFe NPs did not change the secondary structure of HSA. Moreover, UV–visible data presented that melting temperature (Tm) of HSA in the absence and presence of ZVFe NPs was almost identical. Molecular dynamic study also showed that ZVFe NP came into contact with polar residues on the surface of HSA molecule. Cellular assays showed that ZVFe NPs can induce cell mortality in a dose-dependent manner against SH-SY5Y cells, whereas these NPs did not trigger significant cell mortality against normal white bloods in the concentration range studied (1–100 µg/mL). Antibacterial assays showed a noteworthy inhibition on both bacterial strains.ConclusionIn conclusion, it was revealed that ZVFe NPs did not induce a substantial influence on the structure of protein and cytotoxicity against normal cell, whereas they derived significant anticancer and antibacterial effects.

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Section: H Rt S Rmentioning

confidence: 96%

Section: H Rt S Rmentioning

confidence: 96%

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Albumin binding, anticancer and antibacterial properties of synthesized zero valent iron nanoparticles

Anbouhi¹,

Esfidvajani²,

Nemati³

et al. 2018

IJN

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“…Therefore, it is essential to investigate the type of particular interaction in InP/ZnS@BSA conjugates. Hence, thermodynamic parameters are calculated from van't Hoff plots at temperatures 288, 298, 308 and 318 K (see Figure S4a–d). The enthalpy change (Δ H ), entropy change (Δ S ) and Gibbs free energy change (Δ G ) of InP/ZnS@BSA conjugation process are obtained from Equations () and () and results are tabulated in Table .…”

Section: Resultsmentioning

confidence: 99%

Comprehensive study of interaction between biocompatible PEG‐InP/ZnS QDs and bovine serum albumin

et al. 2017

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Polyethylene glycol (PEG) surface modified biocompatible InP/ZnS quantum dots (QDs) act as a potential alternative for conventional carcinogenic cadmium-based quantum dots for in vivo and in vitro studies. Comprehensively, we studied the interaction between a model protein bovine serum albumin (BSA) and PEGylated toxic free InP/ZnS QDs using various spectroscopic tools such as absorption, fluorescence quenching, time resolved and synchronous fluorescence spectroscopic measurements. These studies principally show that tryptophan (Trp) residues of BSA have preferable binding affinity towards PEG-InP/ZnS QDs surface and a blue shift in Trp fluorescence emission is a signature of conformational changes in its hydrophobic microenvironment. Photoluminescence (PL) intensity of Trp is quenched by ground state complex formation (static quenching) at room temperature. However, InP/ZnS@BSA conjugates become unstable with increasing temperature and PL intensity of Trp is quenched via dynamic quenching by PEG-InP/ZnS QDs. Experimentally determined thermodynamic parameters for these conjugates have shown spontaneity, entropy driven and exothermic nature of bio-conjugation. The calculated binding affinity (n ≅ 1, Hill coefficient) suggest that the affinity of InP/ZnS QDs for a BSA protein is not dependent on whether or not other BSA proteins are already bound to the QD surface. Energy transfer efficiency (E), Trp residue to InP/ZnS QDs distances and energy transfer rate (k ) were all obtained from FÖrster resonance energy.

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“…Surface functionalization of NPs can also influence the protein adsorption and subsequent NP-induced conformational changes. Protein surface residues form an interaction with energetically favorite counterparts on the NP surface based on their charge, hydrophobicity, and hydrophilicity [86].…”

Section: The Effect Of Np On the Protein Conformational Changesmentioning

confidence: 99%

Health concerns of various nanoparticles: A review of their in vitro and in vivo toxicity

Ajdari¹,

Moosavi²,

Rahmati³

et al. 2018

Preprint

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Nanoparticles (NPs) are widely used in diverse disciplines, including biology, medicine science. The central question that need to be answered is whether NPs have toxic effects on biological cells and molecules or are they safe. The safety of NPs including targeted drug delivery is critical and so is their toxicity in the environment. In recent years, in vitro and in vivo research on animals has generated abundant information about the toxicity of NPs.However, due to varying laboratory conditions, the comparison of the results from ensuing studies is somewhat unreliable. It should be noted that, depending on the type of production, NPs can enter the body through inhalation, skin and via digestive routes. Due to the diversity of NPs and their properties, there is paucity of accurate information on their toxicological effects; particle size, shape, surface area and the chemical levels are considered as key factors in creating health and toxicological effects. Consequently, there is a need for reliable information about their effects on various organs so as to deal with NPs effectively and their impact on health and the environment. This review covers the existing knowledge base on the subject that hopefully prepares us better to address these challenges.

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Thermodynamic and conformational changes of protein toward interaction with nanoparticles: a spectroscopic overview

Abstract: Nanoparticles (NPs) in different forms have been widely used in medicine and pharmaceutics for diagnosis and drug delivery.

Cited by 94 publications

References 150 publications

Albumin binding, anticancer and antibacterial properties of synthesized zero valent iron nanoparticles

Albumin binding, anticancer and antibacterial properties of synthesized zero valent iron nanoparticles

Comprehensive study of interaction between biocompatible PEG‐InP/ZnS QDs and bovine serum albumin

Health concerns of various nanoparticles: A review of their in vitro and in vivo toxicity

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