Thermodynamic and kinetic properties of interpolymer complexes assessed by isothermal titration calorimetry and surface plasmon resonance

Abstract

Interpolymer complexes (IPCs) formed between complimentary polymers in solution have shown a wide range of applications from drug delivery to biosensors. This work describes the combined use of isothermal titration calorimetry and surface plasmon resonance to investigate the thermodynamic and kinetic processes during hydrogen-bonded interpolymer complexation. Varied polymers that are commonly used in layer-by-layer coatings and pharmaceutical preparations were selected to span a range of chemical functionalities including some known IPCs previously characterized by other techniques, and other polymer combinations with unknown outcomes. This work is the first to comprehensively detail the thermodynamic and kinetic data of hydrogen bonded IPCs, aiding understanding and detailed characterization of the complexes. The applicability of the two techniques in determining thermodynamic, gravimetric and kinetic properties of IPCs is considered.

Probing the Mucoadhesive Interactions Between Porcine Gastric Mucin and Some Water‐Soluble Polymers

“…ITC is a very powerful method to study bimolecular interactions. It has been widely used to study interactions of polymers/biopolymers with other polymers, surfactants, small molecules, etc . It measures molecular interactions in solution in terms of changes in heat, which can be exothermic or endothermic depending on the nature of the interacting species.…”

“…ITC raw data showed the evidence of exothermic interaction between PAA and mucin (Figure S2). Exothermic effects are often associated with hydrogen bonding, which indicates that this could be a predominant nature of mucin–PAA interactions. This conclusion is in good agreement with Patel et al Sonication was found to have a significant ( p < 0.05) effect on the intensity of PAA–mucin interactions; stronger exothermic events are observed for sonicated mucin samples with higher Δ H values (Table ).…”

“…This feature makes mucin–polymer interactions a unique system to study by ITC and shows that the dimensional and physical properties of the mucin sample cannot be ignored. The majority of previous applications of ITC were focused on molecularly dispersed systems such as polymer–polymer complexes, protein–small molecules, DNA/RNA–dendrimer binding, metal ion–small chelate molecules, etc . The recent publications on the use of ITC to study PGM interactions with chitosan or with epigallocatechin gallate either used a soluble and purified fraction of mucin extracted from commercial samples or simply disregarded the colloidal nature of mucin dispersions .…”

Composite Cryogel with Polyelectrolyte Complexes for Growth Factor Delivery

“…The detailed mechanism of interactions between polymers is out of scope of the current study. Previously, the formation of IPC between PVA and negatively charged polymers such as polysaccharide, polyaspartic acids, and polyacrylic acid were reported [54][55][56]. Formed IPC associated with PVA-Hep led to a hindrance of sulfo groups of Hep with CHI, slowing down the formation of PEC at the studied temperature and concentration.…”

Kinetic analysis of IgM monoclonal antibodies for determination of dengue sample concentration using SPR technique

“…22,25,26 Biacore configuration and SPR chip construction Biacore 3000 from GE Healthcare is a real-time system for biomolecular interaction analysis using SPR technique. [27][28][29] With this method it is possible to monitor the formation and dissociation of biomolecular complexes on chip surfaces. CM5 (carboxymethyl dextran matrix) is served as a biochip for this research.…”