Thermodynamic study of the solubility of Guanine and Uracil in deferent aqueous sugar solution

Abstract

We investigate the solubility of guanine and uracil, two nucleic acid bases xylose, glucose, and sucrose solutions in aqueous. It was discovered Guanine solubility rises in lockstep with glucose and sucrose concentrations. However, this is not the case for the other reagents. Guanine solubility increases below 1.5M reagent, and it follows this pattern: ribose(R) <xylose(X) <glucose (Glc) < sucrose(S). The ability to dissolve uracil in these solutions, and another hand, was dependent on the reagent, and it was observed that it decreased, increased, or remained unchanged. The temperature was found to play an important role in terms of the solubility of guanine and uracil in solutions containing sugar, and it was discovered that the transition of these molecules from the sugar solution to the water reaction was exothermic. We studied the solubility of the nucleic acid bases,Guanine and Uracil, in an aqueous sugar solution (glucose, xylose and sucrose)at three different temperatures (5, 25, and 45°C). The value of solubility Sv/So and equilibrium constant (K) were calculated and discussed. Thermodynamic parameters, i.e. change in standard free energy (ΔG 0 ), change in stander entropy(ΔS 0 ), energy transfer(ΔG tr ), and change in standard enthalpy (ΔH 0 )for all these compounds were also calculated and discussed.