Thermogenesis and Brown Adipose Tissue Activity in High Fat Fed Osborne-Mendel Rats

Laury, M C; Goubern, Marc; Razanoelina, M.; Portet, R

doi:10.1159/000177475

Cited by 7 publications

(1 citation statement)

References 16 publications

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“…HFD feeding has been shown to stimulate thermogenesis and BAT activity and to elevate BAT UCP1 levels in rats (12,13,23,33,36). One report in rats showed markedly increased Ucp1 mRNA levels in "whole abdominal WAT" (combined retro-and intraperitoneal, omental and mesenteric depots) following HFD feeding (25).…”

Section: E906mentioning

confidence: 99%

Activation of β₃-adrenoceptors increases in vivo free fatty acid uptake and utilization in brown but not white fat depots in high-fat-fed rats

Warner

Kjellstedt

Carreras

et al. 2016

American Journal of Physiology-Endocrinology and Metabolism

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Activation of brown adipose tissue (BAT) and browning of white adipose tissue (WAT) present potential new therapies for obesity and type 2 diabetes. Here, we examined the effects of β-adrenergic stimulation on tissue-specific uptake and storage of free fatty acids (FFA) and its implications for whole body FFA metabolism in diet-induced obese rats using a multi-radiotracer technique. Male Wistar rats were high fat-fed for 12 wk and administered β3-agonist CL316,243 (CL, 1 mg·kg·day) or saline via osmotic minipumps during the last 3 wk. The rats were then fasted and acutely infused with a tracer mixture ([C]palmitate and the partially metabolized R-[H]bromopalmitate) under anesthesia. CL infusion decreased body weight gain and fasting plasma glucose levels. While core body temperature was unaffected, infrared thermography showed an increase in tail heat dissipation following CL infusion. Interestingly, CL markedly increased both FFA storage and utilization in interscapular and perirenal BAT, whereas the flux of FFA to skeletal muscle was decreased. In this rat model of obesity, only sporadic populations of beige adipocytes were detected in the epididymal WAT depot of CL-infused rats, and there was no change in FFA uptake or utilization in WAT following CL infusion. In summary, β-agonism robustly increased FFA flux to BAT coupled with enhanced utilization. Increased BAT activation most likely drove the increased tail heat dissipation to maintain thermostasis. Our results emphasize the quantitative role of brown fat as the functional target of β-agonism in obesity.

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Section: E906mentioning

confidence: 99%

Activation of β₃-adrenoceptors increases in vivo free fatty acid uptake and utilization in brown but not white fat depots in high-fat-fed rats

Warner

Kjellstedt

Carreras

et al. 2016

American Journal of Physiology-Endocrinology and Metabolism

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Effekt von Palmitin-, Öl- und Linolsäure auf die Thermogenese im Modellversuch an Sauen

Kirchgeßner

1993

Z Ernährungswiss

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An animal model experiment was conducted with nine adult sows to study the effects of long-chain fatty acids on thermogenesis when different fatty acids were replaced for 30% of the energy of a basal diet based on cereals and soybean meal. The acids were fed as commercial products containing as main constituent either palmitic acid, oleic acid, or linoleic acid, according to a latin square design in experimental periods 2 to 4. In periods 1 and 5 the sows were submitted to basal diet alone. Digestibility of palmitic acid was only 36%, whereas the unsaturated fatty acids were highly absorbed (90%). Interaction effects of the undigested proportion of the long-chain fatty acids with the basal diet in hindgut fermentation could be ruled out since a supplementary experiment on three sows showed no influence of infusion of oleic or linoleic acid into the caecum on the energy utilization of the basal diet. There was no significant differences in thermogenesis among the fatty acids. Heat production in the treatment periods averaged -1.2% as compared to the basal diet periods. This result was in accordance with the value -1.0% calculated theoretically for the reduction in heat production in the treatment periods. Thus, the data did not indicate any stimulating effect of long-chain fatty acids on heat production, and utilization of energy of fatty acids occurred within the obligatory thermogenesis.

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Effect of sympathetic de-activation on thermogenic function and membrane lipid composition in mitochondria of brown adipose tissue

Goubern

Chapey

Senault

et al. 1992

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Thermogenesis and Brown Adipose Tissue Activity in High Fat Fed Osborne-Mendel Rats

Cited by 7 publications

References 16 publications

Activation of β₃-adrenoceptors increases in vivo free fatty acid uptake and utilization in brown but not white fat depots in high-fat-fed rats

Activation of β₃-adrenoceptors increases in vivo free fatty acid uptake and utilization in brown but not white fat depots in high-fat-fed rats

Effekt von Palmitin-, Öl- und Linolsäure auf die Thermogenese im Modellversuch an Sauen

Effect of sympathetic de-activation on thermogenic function and membrane lipid composition in mitochondria of brown adipose tissue

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Thermogenesis and Brown Adipose Tissue Activity in High Fat Fed Osborne-Mendel Rats

Cited by 7 publications

References 16 publications

Activation of β3-adrenoceptors increases in vivo free fatty acid uptake and utilization in brown but not white fat depots in high-fat-fed rats

Activation of β3-adrenoceptors increases in vivo free fatty acid uptake and utilization in brown but not white fat depots in high-fat-fed rats

Effekt von Palmitin-, Öl- und Linolsäure auf die Thermogenese im Modellversuch an Sauen

Effect of sympathetic de-activation on thermogenic function and membrane lipid composition in mitochondria of brown adipose tissue

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Activation of β₃-adrenoceptors increases in vivo free fatty acid uptake and utilization in brown but not white fat depots in high-fat-fed rats

Activation of β₃-adrenoceptors increases in vivo free fatty acid uptake and utilization in brown but not white fat depots in high-fat-fed rats