Thermographic assessment of tumor growth in mouse xenografts

Abstract

In human breast tumors, a 1-2°C increase in skin surface temperature is usually observed at the periphery; it has been proposed that this change is due to the hypervascularity and increased blood flow resulting from tumor-associated angiogenesis. Here we tested the hypothesis that thermal imaging might represent a useful adjunctive technique in monitoring the growth dynamics of human tumor xenografts. Xenografts were established in immunocomprised nude mice using MDA-MB-231 or MCF7 breast cancer cells. We exploited the inherent noncontact and noninvasive advantages of infrared thermography to detect skin surface temperature changes. Continuous thermographic investigation was performed to detect and monitor tumor growth in vivo and high resolution digital images were analyzed to measure the tumor temperature dynamics. In contrast to the skin temperature increases associated with human breast cancer, a consistent temperature decrease was found in the xenograft mice. In one case, a smaller secondary tumor, otherwise undetectable, was clearly evident by thermal imaging. The tumors were cooler than the surrounding tissue with a maximum temperature reduction of 1.5°C for MDA-MB-231 tumor and 3°C for MCF7 tumors observed on day 14. In addition, the temperature of the xenograft tumors decreased progressively as they grew throughout the observation period. It was demonstrated that thermographic imaging could detect temperature changes as small as 0.1°C on the skin surface at an early stage of tumor development. The findings of the study indicate that thermographic imaging might have considerable potential in monitoring human tumor xenografts and their response to anticancer drugs. ' 2007 Wiley-Liss, Inc.

Interventional Infrared Thermal Diagnostics in Medicine and Physiology

“…Many medical and physiological IRT-based studies are carried out using small laboratory animals (usually rats and mice) [10][11][12][13][14]. In this respect, considerable difficulties are connected with the following factors: 1) small-sized organs that require high spatial resolution of IRT equipment; 2) high mobility of non-narcotized animals that requires the use of IR camera of high operation speed; 3) hair-covered body whose hair is always in a good contact with air and, at high sensitivity of IRT method, it leads to the appearance of considerable temperature artifacts caused by air motion in a laboratory.…”

Adeno-associated virus type 2 infection of nude mouse human breast cancer xenograft induces necrotic death and inhibits tumor growth

“…Since the xenograft tumors develop volumetric shapes which are roughly elliptical, tumor volume (V ) was determined by the equation: V = π(w + l) 3 / 48, where l is the length and w is the width of the tumor, measured with calipers without applying compression, as published elsewhere. 63 When xenografts reached volumes of ~300 mm 3 (approximately 2 wk), the mice were randomly assigned to control and AAV2 treated groups (n = 5). Two sets of 5 mice each received a single AAV2 dosage of 10 5 and 10 6 infectious units per tumor administered via intratumoral injections.…”

<b>Estudo crítico de mastocitomas caninos e avaliação termográfica de técnicas de anaplastia</b>

“…Muitos autores descrevem a termografia como um método diagnóstico seguro, preciso e não invasivo; aprovado para uso em Medicina Humana e em especial para avaliação de crescimento tumoral (JIANG et al, 2005;MIKULSKA, 2006;SONG et al, 2007;ARORA et al, 2008 Estudos termográficos clássicos sugerem que uma imagem infravermelha típica de um tumor da mama in vivo revela 1-3 ºC de elevação da temperatura da pele na periferia do tumor (GAUTHERIE et al, 1980) e elevação de 0,8 a 2ºC na mama contralateral a afetada (LAWSON, 1956). Yahara et al (2003) demonstraram que a temperatura de tumores mamários de mulheres foi significativamente maior do que a do tecido ao redor e sugeriu uma relação entre a temperatura tumoral e o prognóstico do tumor de mama.…”

“…Yahara et al (2003) demonstraram que a temperatura de tumores mamários de mulheres foi significativamente maior do que a do tecido ao redor e sugeriu uma relação entre a temperatura tumoral e o prognóstico do tumor de mama. Estudos com o uso de xenoenxertos para pesquisa do carcinoma em mulheres (SONG et al, 2007;POLJAK-BLAZI et al, 2009) descrevem a variação de temperatura entre a pele e o tumor como sendo negativa, e estabelecem que embora seja evidenciada a elevação da temperatura da superfície da pele na periferia imediata do tumor, a massa tumoral em si, em alguns momentos de sua evolução, mostra redução da temperatura em relação a pele sadia. A causa destas mudanças de temperatura não é totalmente compreendida mas sugere-se que seja associada a neoangiogênese local (XIE et al, 2004).…”

“…A causa destas mudanças de temperatura não é totalmente compreendida mas sugere-se que seja associada a neoangiogênese local (XIE et al, 2004). 2 REVISÃO DE LITERATURA A quase totalidade dos estudos atualmente publicados que associam termografia e oncologia humana tem sido representada pela avaliação da ferramenta no câncer de mama em mulheres (YAHARA et al, 2003;SONG et al, 2007;ARORA et al, 2008;WISHART et al, 2010;KONTOS et al, 2011;WANG et al, 2011;BOQUETE et al, 2012). Os estudos mais recentes ainda concluem que esta é uma técnica de boa reptibilidade e concordância intra e inter observadores, além de ser não invasiva e fácil uso (GARDUÑO-RAMÓN et al, 2017 …”