Thermohydrogenium kirishiense gen. nov. and sp. nov., a new anaerobic thermophilic bacterium

Zacharova, E. V.; Mitrofanova, T. I.; Krasil’nikova, E. N.; Kondratieva, E. N.

doi:10.1007/bf00245311

Cited by 10 publications

(1 citation statement)

References 13 publications

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“…These bacteria use several sugars, starch and yeast extract for growth, and the main fermentation products from glucose are ethanol, acetate, H 2 and CO 2 . Minor concentrations of isopropanol, butanol, butyrate and lactate can be produced by the bacteria (Zacharova et al 1993). In this study, the presence of yeast extract in the culture medium may have favored the development of these bacteria.…”

Section: High Abundance Of Thermoanaerobacterium and Thermohydrogeniummentioning

confidence: 92%

Bacterial communities in thermophilic H2-producing reactors investigated using 16S rRNA 454 pyrosequencing

Ratti

Delforno

Okada

et al. 2015

Microbiological Research

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In this study, the composition and diversity of the bacterial community in thermophilic H2-producing reactors fed with glucose were investigated using pyrosequencing. The H2-producing experiments in batch were conducted using 0.5 and 2.0 g l(-1) glucose at 550 °C. Under the two conditions, the H2 production and yield were 1.3 and 1.6 mol H2 mol glucose(-1), respectively. Acetic, butyric, iso-butyric, lactic and propionic acids were detected in the two reactors. The increase in substrate concentration favored a high H2 yield. In this reactor, a predominance of acetic and iso-butyric acids, 27.7% and 40%, were measured, respectively. By means of pyrosequencing, a total of 323 and 247 operational taxonomic units were obtained, with a predominance of the phylum Firmicutes (68.73-67.61%) for reactors with 0.5 and 2.0 g l(-1) glucose, respectively. Approximately 40.55% and 62.34% of sequences were affiliated with Thermoanaerobacterium and Thermohydrogenium, microorganisms that produce H2 under thermophilic conditions.

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Section: High Abundance Of Thermoanaerobacterium and Thermohydrogeniummentioning

confidence: 92%

Bacterial communities in thermophilic H2-producing reactors investigated using 16S rRNA 454 pyrosequencing

Ratti

Delforno

Okada

et al. 2015

Microbiological Research

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Elena N. Kondratieva (1925–1995)

1996

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Enhancement of acetate productivity in a thermophilic (55 °C) hollow-fiber membrane biofilm reactor with mixed culture syngas (H2/CO2) fermentation

Wang

Zhang

et al. 2017

Appl Microbiol Biotechnol

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Conversion of organic wastes to syngas is an attractive way to utilize wastes. The produced syngas can be further used to produce a variety of chemicals. In this study, a hollow-fiber membrane biofilm reactor with mix cultures was operated at 55 °C to convert syngas (H/CO) into acetate. A high concentration of acetate (42.4 g/L) was reached in batch experiment while a maximum acetate production rate of 10.5 g/L/day was achieved in the continuous-flow mode at hydraulic retention time (HRT) of 1 day. Acetate was the main product in both batch and continuous-flow experiments. n-Butyrate was the other byproduct in the reactor. Acetate accounted for more than 98.5 and 99.1% of total volatile fatty acids in batch and continuous modes, respectively. Illumina Miseq high-throughput sequencing results showed that microorganisms were highly purified and enriched in the reactor. The main genus was Thermoanaerobacterium (66% of relative abundance), which was usually considered as H producer in the literature, however, likely played a role as a H consumer in this study. This study provides a new method to generate the high producing rate and purity of acetate from syngas.

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Thermohydrogenium kirishiense gen. nov. and sp. nov., a new anaerobic thermophilic bacterium

Cited by 10 publications

References 13 publications

Bacterial communities in thermophilic H2-producing reactors investigated using 16S rRNA 454 pyrosequencing

Bacterial communities in thermophilic H2-producing reactors investigated using 16S rRNA 454 pyrosequencing

Elena N. Kondratieva (1925–1995)

Enhancement of acetate productivity in a thermophilic (55 °C) hollow-fiber membrane biofilm reactor with mixed culture syngas (H2/CO2) fermentation

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