Thermoluminescence as a complementary technique for the toxicological evaluation of chemicals in photosynthetic organisms

Repetto, Guillermo; Zurita, Jorge L.; Roncel, Mercedes; Ortega, J.

doi:10.1016/j.aquatox.2014.11.002

Cited by 9 publications

(11 citation statements)

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“…Thermoluminescence glow curves of P. tricornutum cell suspensions were obtained using an home-built apparatus designed by Dr. Jean-Marc Ducruet (France) for luminescence detection from 1 to 80°C (standard thermoluminescence, STL) and from 10 to 160°C (high temperature thermoluminescence, HTL). A detailed description of the system can be obtained elsewhere ( Ducruet, 2003 ; Zurita et al, 2005 ; Guerrero et al, 2014 ; Repetto et al, 2015 ). Briefly, temperature regulation, signal recording and flash sequences were driven by a computer through a National Instrument DAQ-Pad 1200 interface, using a specially developed acquisition program ( Ducruet, 2003 ).…”

Section: Methodsmentioning

confidence: 99%

Iron Deficiency Induces a Partial Inhibition of the Photosynthetic Electron Transport and a High Sensitivity to Light in the Diatom Phaeodactylum tricornutum

et al. 2016

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Iron limitation is the major factor controlling phytoplankton growth in vast regions of the contemporary oceans. In this study, a combination of thermoluminescence (TL), chlorophyll fluorescence, and P700 absorbance measurements have been used to elucidate the effects of iron deficiency in the photosynthetic electron transport of the marine diatom P. tricornutum. TL was used to determine the effects of iron deficiency on photosystem II (PSII) activity. Excitation of iron-replete P. tricornutum cells with single turn-over flashes induced the appearance of TL glow curves with two components with different peaks of temperature and contributions to the total signal intensity: the B band (23°C, 63%), and the AG band (40°C, 37%). Iron limitation did not significantly alter these bands, but induced a decrease of the total TL signal. Far red excitation did not increase the amount of the AG band in iron-limited cells, as observed for iron-replete cells. The effect of iron deficiency on the photosystem I (PSI) activity was also examined by measuring the changes in P700 redox state during illumination. The electron donation to PSI was substantially reduced in iron-deficient cells. This could be related with the important decline on cytochrome c6 content observed in these cells. Iron deficiency also induced a marked increase in light sensitivity in P. tricornutum cells. A drastic increase in the level of peroxidation of chloroplast lipids was detected in iron-deficient cells even when grown under standard conditions at low light intensity. Illumination with a light intensity of 300 μE m-2 s-1 during different time periods caused a dramatic disappearance in TL signal in cells grown under low iron concentration, this treatment not affecting to the signal in iron-replete cells. The results of this work suggest that iron deficiency induces partial blocking of the electron transfer between PSII and PSI, due to a lower concentration of the electron donor cytochrome c6. This decreased electron transfer may induce the over-reduction of the plastoquinone pool and consequently the appearance of acceptor side photoinhibition in PSII even at low light intensities. The functionality of chlororespiratory electron transfer pathway under iron restricted conditions is also discussed.

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Section: Methodsmentioning

confidence: 99%

Iron Deficiency Induces a Partial Inhibition of the Photosynthetic Electron Transport and a High Sensitivity to Light in the Diatom Phaeodactylum tricornutum

et al. 2016

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“…Thermoluminescence (TL) glow curves of L. japonicus plants were obtained using a home‐built apparatus (University of Seville‐CSIC, Spain) designed by Dr Ducruet (Université Paris‐Sud‐Orsay, Orsay, France) for luminescence detection from 1 to 70°C (standard thermoluminescence, STL) and from 10 to 160°C (high temperature thermoluminescence, HTL). A detailed description of the system can be obtained elsewhere (Ducruet , Zurita et al , Sajnani et al , Guerrero et al , Repetto et al ). Data acquisition, signal analysis and graphical simulation were performed as previously described (Ducruet , Zurita et al ).…”

Section: Methodsmentioning

confidence: 99%

The afterglow thermoluminescence band as an indicator of changes in the photorespiratory metabolism of the model legumeLotus japonicus

García‐Calderón

Betti

Márquez

et al. 2019

Physiologia Plantarum

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The afterglow (AG) luminescence is a delayed chlorophyll fluorescence emitted by the photosystem II that seems to reflect the level of assimilatory potential (NADPH+ATP) in chloroplast. In this work, the thermoluminescence (TL) emissions corresponding to the AG band were investigated in plants of the WT and the Ljgln2‐2 photorespiratory mutant from Lotus japonicus grown under either photorespiratory (air) or non‐photorespiratory (high concentration of CO2) conditions. TL glow curves obtained after two flashes induced the strongest overall TL emissions, which could be decomposed in two components: B band (tmax = 27–29°C) and AG band (tmax = 44–45°C). Under photorespiratory conditions, WT plants showed a ratio of 1.17 between the intensity of the AG and B bands (IAG/IB). This ratio increased considerably under non‐photorespiratory conditions (2.12). In contrast, mutant Ljgln2‐2 plants grown under both conditions showed a high IAG/IB ratio, similar to that of WT plants grown under non‐photorespiratory conditions. In addition, high temperature thermoluminescence (HTL) emissions associated to lipid peroxidation were also recorded. WT and Ljgln2‐2 mutant plants grown under photorespiratory conditions showed both a significant HTL band, which increased significantly under non‐photorespiratory conditions. The results of this work indicate that changes in the amplitude of IAG/IB ratio could be used as an in vivo indicator of alteration in the level of photorespiratory metabolism in L. japonicus chloroplasts. Moreover, the HTL results suggest that photorespiration plays some role in the protection of the chloroplast against lipid peroxidation.

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“…Irradiations must be carried out at a low temperature and in a vacuum, and thus a window (typically a thin Be window) must be mounted on the cryostat. TL is also used for the study of photosynthesis in intact leaves, and algal and cyanobacterial cells [44,45]. These studies require a very specific instrumental feature: the possibility to cool the sample well below 0 °C, while a vacuum is not possible [46].…”

Section: Instrumentationmentioning

confidence: 99%

“…The radiative route is the one with the largest activation energy and is thus almost absent at a low temperature. Since its discovery, the origin of many TL components have been identified, characterised, and assigned to the redox components of the Photosystem II, which makes TL a versatile tool in photosynthesis research [45,120,121]. …”

Section: Thermoluminescence As a Research Toolmentioning

confidence: 99%

Thermoluminescence as a Research Tool to Investigate Luminescence Mechanisms

Bos

2017

Materials

231

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Thermally stimulated luminescence (TSL) is known as a technique used in radiation dosimetry and dating. However, since the luminescence is very sensitive to the defects in a solid, it can also be used in material research. In this review, it is shown how TSL can be used as a research tool to investigate luminescent characteristics and underlying luminescent mechanisms. First, some basic characteristics and a theoretical background of the phenomenon are given. Next, methods and difficulties in extracting trapping parameters are addressed. Then, the instrumentation needed to measure the luminescence, both as a function of temperature and wavelength, is described. Finally, a series of very diverse examples is given to illustrate how TSL has been used in the determination of energy levels of defects, in the research of persistent luminescence phosphors, and in phenomena like band gap engineering, tunnelling, photosynthesis, and thermal quenching. It is concluded that in the field of luminescence spectroscopy, thermally stimulated luminescence has proven to be an experimental technique with unique properties to study defects in solids.

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Thermoluminescence as a complementary technique for the toxicological evaluation of chemicals in photosynthetic organisms

Abstract: Abbreviations: B-band: TL band due to S 2/3 Q B -recombination; BrB: bromobenzene; C-band:

Cited by 9 publications

References 79 publications

Iron Deficiency Induces a Partial Inhibition of the Photosynthetic Electron Transport and a High Sensitivity to Light in the Diatom Phaeodactylum tricornutum

Iron Deficiency Induces a Partial Inhibition of the Photosynthetic Electron Transport and a High Sensitivity to Light in the Diatom Phaeodactylum tricornutum

The afterglow thermoluminescence band as an indicator of changes in the photorespiratory metabolism of the model legumeLotus japonicus

Thermoluminescence as a Research Tool to Investigate Luminescence Mechanisms

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