Thermoluminescence

Sane, P. V.; Ivanov, Alexander G.; Öquist, Gunnar; Hüner, Norman P. A.

doi:10.1007/978-94-007-1579-0_19

Cited by 15 publications

(21 citation statements)

References 135 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The best characterized TL band is the B-band. Illumination of a single turnover flash with the plant sample after a short dark adaptation induces a major B-band, which appears at ~30–35 ºC (Misra et al ., 2001; Ducruet and Vass, 2009; Sane et al ., 2012). It is correlated with the activity of the oxygen-evolving complex (OEC).…”

Section: Resultsmentioning

confidence: 99%

“…3c; Inoue, 1996). When the dark-adapted PSII centre is exposed to a series of short turnover flashes, formation of the redox pairs: S 2 Q B – , S 2 /S 3 Q B – , and S 3 Q B – is expected after one, two, and three flashes, respectively (Sane et al ., 2012). It was observed that after two turnover flashes, the transformation of the S 2 Q B – charge pairs to S 2 /S 3 Q B – was inhibited in ics1 in comparison with the WT (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…It is assumed that the dark-adapted samples contain ~25% S 0 and 75% S 1 , and the distribution of Q B and Q B – has been shown to be 50:50 (Inoue, 1996). According to the previously described model (Inoue, 1996; Sane et al ., 2012), in the dark-adapted samples the proportion of charge pairs is predicted as 25% S 0 /Q B /Q B – and 75% S 1 /Q B /Q B – . After first saturating, the turnover flash Q B is converted to Q B – and Q B – to Q B 2– .…”

Section: Discussionmentioning

confidence: 99%

“…After two-electron reduction, Q B 2– is transformed into a protonated form (Q B H 2 ), which is replaced by a new Q B from the reoxidized PQ pool. After two turnover flashes, the TL signal originates predominantly from S 3 Q B – (the lower intensity of TL is also emitted by the S 2 Q B – charge pair) (Sane et al ., 2012). The decreased amplitude of the B-band in the ics1 mutant after two turnover flashes strongly suggests that the PQ pool which provides Q B at the acceptor side of PSII (the quinone reduction cycle) is more reduced than in the WT (Fig.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Isochorismate synthase 1 is required for thylakoid organization, optimal plastoquinone redox status, and state transitions in Arabidopsis thaliana

Gawroński

Górecka

Bederska

et al. 2013

Journal of Experimental Botany

View full text Add to dashboard Cite

Isochorismate synthase 1 (ICS1) is a crucial enzyme in the salicylic acid (SA) synthesis pathway, and thus it is important for immune defences. The ics1 mutant is used in experiments on plant–pathogen interactions, and ICS1 is required for the appropriate hypersensitive disease defence response. However, ICS1 also takes part in the synthesis of phylloquinone, which is incorporated into photosystem I and is an important component of photosynthetic electron transport in plants. Therefore, photosynthetic and molecular analysis of the ics1 mutant in comparison with wild-type and SA-degrading transgenic NahG Arabidopsis thaliana plants was performed. Photosynthetic parameters in the ics1 mutant, when compared with the wild type, were changed in a manner observed previously for state transition-impaired plants (STN7 kinase recessive mutant, stn7). In contrast to stn7, deregulation of the redox status of the plastoquinone pool (measured as 1–q p) in ics1 showed significant variation depending on the leaf age. SA-degrading transgenic NahG plants targeted to the cytoplasm or chloroplasts displayed normal (wild-type-like) state transition. However, ics1 plants treated with a phylloquinone precursor displayed symptoms of phenotypic reversion towards the wild type. ics1 also showed altered thylakoid structure with an increased number of stacked thylakoids per granum which indicates the role of ICS1 in regulation of state transition. The results presented here suggest the role of ICS1 in integration of the chloroplast ultrastructure, the redox status of the plastoquinone pool, and organization of the photosystems, which all are important for optimal immune defence and light acclimatory responses.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Isochorismate synthase 1 is required for thylakoid organization, optimal plastoquinone redox status, and state transitions in Arabidopsis thaliana

Gawroński

Górecka

Bederska

et al. 2013

Journal of Experimental Botany

View full text Add to dashboard Cite

show abstract

“…Dark-adapted leaves (20 minutes at 20 °C) were cooled to 2 °C prior to exposing to the flashes. The nomenclature of Sane et al (2012) was used for characterization of the TL glow peaks.…”

Section: Methodsmentioning

confidence: 99%

Excess manganese differentially inhibits photosystem I versus II inArabidopsis thaliana

Millaleo

Reyes‐Díaz

Alberdi

et al. 2012

EXBOTJ

129

View full text Add to dashboard Cite

The effects of exposure to increasing manganese concentrations (50–1500 µM) from the start of the experiment on the functional performance of photosystem II (PSII) and photosystem I (PSI) and photosynthetic apparatus composition of Arabidopsis thaliana were compared. In agreement with earlier studies, excess Mn caused minimal changes in the PSII photochemical efficiency measured as Fv/Fm, although the characteristic peak temperature of the S2/3QB – charge recombinations was shifted to lower temperatures at the highest Mn concentration. SDS-PAGE and immunoblot analyses also did not exhibit any significant change in the relative abundance of PSII-associated polypeptides: PSII reaction centre protein D1, Lhcb1 (major light-harvesting protein of LHCII complex), and PsbO (OEC33, a 33kDa protein of the oxygen-evolving complex). In addition, the abundance of Rubisco also did not change with Mn treatments. However, plants grown under excess Mn exhibited increased susceptibility to PSII photoinhibition. In contrast, in vivo measurements of the redox transients of PSI reaction centre (P700) showed a considerable gradual decrease in the extent of P700 photooxidation (P700+) under increased Mn concentrations compared to control. This was accompanied by a slower rate of P700+ re-reduction indicating a downregulation of the PSI-dependent cyclic electron flow. The abundance of PSI reaction centre polypeptides (PsaA and PsaB) in plants under the highest Mn concentration was also significantly lower compared to the control. The results demonstrate for the first time that PSI is the major target of Mn toxicity within the photosynthetic apparatus of Arabidopsis plants. The possible involvement mechanisms of Mn toxicity targeting specifically PSI are discussed.

show abstract