Currently, most cities landfill most waste glass, resulting in the waste of resources and environmental pollution. Therefore, to realize the recycling of waste glass, solid waste glass was recycled and broken. Waste glass sand was prepared according to the gradation of natural river sand particles and the fineness modulus screening. It was used as an alternative material to natural river sand and mixed with mortar materials with different replacements. Analysis of the mortar with different replacements (0%, 20%, 40%, 60%, 80%) was conducted by combining macro and micro tests on the change law and influence mechanism of permeability, mechanical properties, and microstructure. The results showed that: the replacement of waste glass sand effectively improved the gas permeation resistance of mortar; with the increase of replacement, the gas permeation resistance of mortar roughly showed a trend of increasing first and then decreasing. The replacement of waste glass sand at 20% can better promote cement’s hydration so that the mortar’s porosity is reduced by 16.5%. The gas permeability decreases by 57.4%; the compressive strength increases by 3%, and the elastic modulus increases by 5.9%. When the replacement rate of glass sand is 20%, the test performance of mortar is the best among the five groups.