Thermoplasmonic ssDNA Dynamic Release from Gold Nanoparticles Examined with Advanced Fluorescence Microscopy

Simoncelli, Sabrina; Weerasekera, Hasitha de Alwis; Fasciani, Chiara; Boddy, Christopher N.; Aramendı́a, Pedro F.; Alarcon, Emilio I.; Scaiano, Juan C.

doi:10.1021/acs.jpclett.5b00272

Cited by 12 publications

(10 citation statements)

References 23 publications

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“…The photothermal effect of nanoparticles, whereby light is absorbed and converted to thermal energy, has emerged as an attractive option for driving thermally-activated physical and chemical transformations at greatly enhanced rates [ 1 , 2 , 3 ]. For strongly-absorbing and weakly-emissive nanoparticle systems, it is possible to use widely-available light sources to bring the nanoparticles to extreme temperatures [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Photothermal Effectiveness of Magnetite Nanoparticles: Dependence upon Particle Size Probed by Experiment and Simulation

2018

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The photothermal effect of nanoparticles has proven efficient for driving diverse physical and chemical processes; however, we know of no study addressing the dependence of efficacy on nanoparticle size. Herein, we report on the photothermal effect of three different sizes (5.5 nm, 10 nm and 15 nm in diameter) of magnetite nanoparticles (MNP) driving the decomposition of poly(propylene carbonate) (PPC). We find that the chemical effectiveness of the photothermal effect is positively correlated with particle volume. Numerical simulations of the photothermal heating of PPC supports this observation, showing that larger particles are able to heat larger volumes of PPC for longer periods of time. The increased heating duration is likely due to increased heat capacity, which is why the volume of the particle functions as a ready guide for the photothermal efficacy.

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Section: Introductionmentioning

confidence: 99%

Photothermal Effectiveness of Magnetite Nanoparticles: Dependence upon Particle Size Probed by Experiment and Simulation

2018

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“…27,28 Plasmonic release of oligonucleotides has been studied from the surface of colloidal gold nanoparticles. 29–32 Previously, we have investigated the kinetics of photoactivated controlled-release of miRNA from the surface of colloidal gold nanoparticles using time-resolved second harmonic generation (SHG). 33 …”

Section: Introductionmentioning

confidence: 99%

Plasmon-Enhanced Photocleaving Dynamics in Colloidal MicroRNA-Functionalized Silver Nanoparticles Monitored with Second Harmonic Generation

Kumal¹,

Abu‐Laban²,

Landry³

et al. 2016

Langmuir

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The photocleaving dynamics of colloidal micro-RNA-functionalized nanoparticles are studied using time-dependent second harmonic generation (SHG) measurements. Model drug-delivery systems composed of oligonucleotides attached to either silver nanoparticles or polystyrene nanoparticles using a nitrobenzyl photocleavable linker are prepared and characterized. The photoactivated controlled release is observed to be most efficient on resonance at 365 nm irradiation, with pseudo-first-order rate constants that are linearly proportional to irradiation powers. Additionally, silver nanoparticles show a 6-fold plasmon enhancement in photocleaving efficiency over corresponding polystyrene nanoparticle rates, while our previous measurements on gold nanoparticles show a 2-fold plasmon enhancement compared to polystyrene nanoparticles. Characterizations including extinction spectroscopy, electrophoretic mobility, and fluorimetry measurements confirm the analysis from the SHG results. The real-time SHG measurements are shown to be a highly sensitive method for investigating plasmon-enhanced photocleaving dynamics in model drug delivery systems.

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“…The nano‐localized heating through plasmonic photothermal effect has been reported recently, [ 12–14 ] for controlling chemical reactions [ 15 ] or releasing DNA conjugated on Au nanoparticles surface. [ 16–18 ] However, to the best of our knowledge, there is no report describing the NA amplification through nano‐localized heating around Au nanoparticles, possibly due to two challenges. The first one is that the NA amplification requires precise measurement and control of the nano‐localized temperature field around Au nanoparticles, which is technically challenging for now.…”

Section: Introductionmentioning

confidence: 99%

Quantifying and Adjusting Plasmon‐Driven Nano‐Localized Temperature Field around Gold Nanorods for Nucleic Acids Amplification

You

Jia

et al. 2021

Small Methods

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Fast nucleic acid (NA) amplification has found widespread biomedical applications, where high thermocycling rate is the key. The plasmon‐driven nano‐localized thermocycling around the gold nanorods (AuNRs) is a promising alternative, as the significantly reduced reaction volume enables a rapid temperature response. However, quantifying and adjusting the nano‐localized temperature field remains challenging for now. Herein, a simple method is developed to quantify and adjust the nano‐localized temperature field around AuNRs by combining experimental measurement and numerical simulation. An indirect method to measure the surface temperature of AuNRs is first developed by utilizing the temperature‐dependent stability of Authiol bond. Meanwhile, the relationship of AuNRs′ surface temperature with the AuNRs concentration and laser intensity, is also studied. In combination with thermal diffusion simulation, the nano‐localized temperature field under the laser irradiation is obtained. The results show that the restricted reaction volume (≈aL level) enables ultrafast thermocycling rate (>104 °C s−1). At last, a duplex‐specific nuclease (DSN)‐mediated isothermal amplification is successfully demonstrated within the nano‐localized temperature field. It is envisioned that the developed method for quantifying and adjusting the nano‐localized temperature field around AuNRs is adaptive for various noble metal nanostructures and will facilitate the development of the biochemical reaction in the nano‐localized environment.

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Thermoplasmonic ssDNA Dynamic Release from Gold Nanoparticles Examined with Advanced Fluorescence Microscopy

Cited by 12 publications

References 23 publications

Photothermal Effectiveness of Magnetite Nanoparticles: Dependence upon Particle Size Probed by Experiment and Simulation

Photothermal Effectiveness of Magnetite Nanoparticles: Dependence upon Particle Size Probed by Experiment and Simulation

Plasmon-Enhanced Photocleaving Dynamics in Colloidal MicroRNA-Functionalized Silver Nanoparticles Monitored with Second Harmonic Generation

Quantifying and Adjusting Plasmon‐Driven Nano‐Localized Temperature Field around Gold Nanorods for Nucleic Acids Amplification

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